... and plasmid were analyzed using linearized pBAD ⁄ gIII plasmid, linearized and denatured plasmid and intact plasmid The pBAD ⁄ gIII plasmid was linearized using SalI and denatured by incubation ... Biochemical Adaptation Princeton University Press, Princeton, NJ Wu SI, Lo SK, Shao CP, Tsai HW & Hor LI (2001) Cloning and characterization of a periplasmic nuclease of Vibrio vuln...
Ngày tải lên: 19/02/2014, 05:20
Tài liệu Báo cáo khoa học: Distribution of class I, III and IV alcohol dehydrogenase mRNAs in the adult rat, mouse and human brain ppt
... involvement of ADH1 and ADH4 in ethanol oxidation in brain tissue Regarding retinoid metabolism in the adult brain, enzymes other than ADH4 must be active, because in vivo and in vitro data indicate ... Table Distribution of the three ADH classes in adult rat, mouse and human brain tissue The presence of specific signal is shown by +, strong prese...
Ngày tải lên: 21/02/2014, 00:20
Tài liệu Báo cáo khoa học: Identi®cation and properties of type I-signal peptidases of Bacillus amyloliquefaciens doc
... Cloning of sipU Cloning of sipU Cloning of sipU Cloning of sipU Cloning of sipU Cloning of sipU Cloning of sipU Cloning of sipU Cloning of sipV Cloning of sipV Cloning of sipV Cloning of sipV ... Cloning of sipV Cloning of sipV Cloning of sipV Construction of pOpacVh Construction of pOpacVh Cloning of sipW Cloning of sipW Cloning of sipW Cloning of si...
Ngày tải lên: 21/02/2014, 03:20
Báo cáo Y học: Properties of group I allergens from grass pollen and their relation to cathepsin B, a member of the C1 family of cysteine proteinases pdf
... resistant to cleavage with chymotrypsin, trypsin, papain, or pronase Protozoan parasites of the genus Giardia are one of the earliest lineages of eukaryotic cells, and the Giardia protease is the ... expressed at a high level (data not shown) DISCUSSION Computer analysis of b-expansins reveals significant similarity to cathepsins, which are members of the C1 fami...
Ngày tải lên: 08/03/2014, 22:20
Báo cáo khoa học: Effects of salt on the kinetics and thermodynamic stability of endonuclease I from Vibrio salmonicida and Vibrio cholerae potx
... stoichiometries of participation of water, cations and anions in specific and non-specific binding of lac repressor to DNA Possible thermodynamic origins of the ‘‘glutamate effect’’ on protein–DNA ... function optimally Effects of mutations The point mutations are not in the immediate vicinity of the active site situated at the bottom of the positively charge...
Ngày tải lên: 16/03/2014, 06:20
Báo cáo khoa học: Knock-out of the chloroplast-encoded PSI-J subunit of photosystem I in Nicotiana tabacum PSI-J is required for efficient electron transfer and stable accumulation of photosystem I pot
... and the other for integrity and stabilization of a luminal domain involving at least PSI-N and the N-terminal part of PSI-F which is required for efficient electron transfer Fig Alignment of PSI-J ... 2), suggesting that the PSI antenna size is unaffected by the elimination of PSI-J and furthermore ruling out the possibility that PSI-J is stric...
Ngày tải lên: 16/03/2014, 11:20
Báo cáo khoa học: Comparative biochemical and functional studies of family I soluble inorganic pyrophosphatases from photosynthetic bacteria potx
... rans phil us Vibrio cholerae Escherichia coli Rickettsia prowazekii Sulfolobus acidocaldarius Aquifex aeolicus Helicobacter pylori B ac Prokaryotic Family I sPPases * Neisseria meningitidis 925 ... (MDLSRIPPQP KAGILNVLIE IPAG), and Rhodop viridis (MRIDA IDXA), and that of Mi aeruginosa NIES-44 (MDL SRKPAQP IPGLKNVLVE TAGSINIT) [16], show a high degree of similarity with other cyto...
Ngày tải lên: 16/03/2014, 11:20
Báo cáo khoa học: Characterization of D-amino-acid-containing excitatory conotoxins and redefinition of the I-conotoxin superfamily pot
... wells and At the same time, the activity of the muscle was recorded with a pair of electrodes: one electrode was located in the middle, and the other at one end, of the trough Each pair of recording ... from amino-acid sequences Purification and synthesis of I -superfamily conotoxins D-Amino The excitatory peptides r11b and r11c were purified from the veno...
Ngày tải lên: 16/03/2014, 22:20
Báo cáo khoa học: Making sense of G-quadruplex and i-motif functions in oncogene promoters pot
... contaminating protein that is either an accessory protein or a minor recombinant protein [34] Studies show that an R88A mutation (arginine to alanine) in the nucleotidebinding site eliminates binding ... G-quadruplex and i-motif in oncogene promoters T A Brooks et al enrichment of these motifs in oncogenes Consistent with this finding, G-quadruplex motifs within several on...
Ngày tải lên: 23/03/2014, 03:20
Báo cáo khoa học: A comparative study of type I and type II tryparedoxin peroxidases in Leishmania major pot
... TATATCATATGTCTATCTACGACTTCAAGGTC ATATAGGATCCTCACGATTGAGTGCTTGG ATATATCATATGTCCGGTGTCGCAAAG ATATAGGATCCTTACTCGTCTCTCCACGG ATATATCATATGTCCTGCGGTAACGCC ATATAGGATCCTTACTGCTTGCTGAAGTATC CAACGTAGCCAGCAAGGCCGGCTTCACCAAGGGCG ... Heterologous priming-boosting with DNA and modied vaccinia virus Ankara expressing tryparedoxin peroxidase promotes long-term memory against Leishmania major in sus...
Ngày tải lên: 23/03/2014, 07:20
Báo cáo khoa học: Signalling and regulation of collagen I synthesis by ET-1 and TGF-b1 doc
... values TGF-b1 signalling was affected similarly, with no effect by inhibiting PLC-b (Fig 5A), strong down -regulation of collagen I levels after inhibition of PC-PLC (Fig 5B), and similar collagen I ... subunit of Gai [45] was inhibited specifically by U73122 [46] Inhibiting PLC-b by U73122 did not alter ET-1- induced collagen I synthesis, nor did it influence b...
Ngày tải lên: 23/03/2014, 11:20
Báo cáo hóa học: " Simultaneous circulation of genotypes I and III of dengue virus 3 in Colombia" docx
... Ecuador00b I I I I I I I I I I I I I I I I I I I I I I (V)b I (V)b I (V)b II II II II II II II II II II II II II II II III III III III III III III III III III III III III III III III III III III III 1998 ... Asia/S.Pacific (I) India (III) India (III) India (III) India (III) India (III) India (III) India (III) India (III) India (III) India (III) In...
Ngày tải lên: 20/06/2014, 01:20
Financial Audit of the Department of Agriculture A Report to the Governor and the Legislature of the State of Hawai`i Report No. 05-02 April 2005_part1 docx
... The Auditor State of Hawai`i OVERVIEW Financial Audit of the Department of Agriculture Report No 05-02, April 2005 Summary The Office of the Auditor and the certified public accounting firm of ... Grant Thornton LLP conducted a financial audit of the Department of Agriculture, State of Hawai`i, for the fiscal year July 1, 2003 to...
Ngày tải lên: 20/06/2014, 02:20