... cultures, using brassinin as the substrate, to monitor the enzymatic activity The purified enzymes were designated as BHLmL2 for the BH from L maculans L2 ⁄ M2, and as BHAb for the BH from A brassicicola ... cultures of Leptosphaeria maculans (A) and Alternaria brassicicola (B) The results are expressed as means and standard deviations of three independent experiments 7414 The purity of BHLmL2 obtained from ... 72% and 85% inhibition of BHLmL2 and BHAb, respectively, while mm 2-mer- captoethanol caused 85% and 97% inhibition of BHLmL2 and BHAb, respectively The influence of pH on the activities of the...