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11590 the city and the country

Tài liệu Báo cáo khoa học: Substrate specificity and inhibition of brassinin hydrolases, detoxifying enzymes from the plant pathogens Leptosphaeria maculans and Alternaria brassicicola ppt

Tài liệu Báo cáo khoa học: Substrate specificity and inhibition of brassinin hydrolases, detoxifying enzymes from the plant pathogens Leptosphaeria maculans and Alternaria brassicicola ppt

... cultures, using brassinin as the substrate, to monitor the enzymatic activity The purified enzymes were designated as BHLmL2 for the BH from L maculans L2 ⁄ M2, and as BHAb for the BH from A brassicicola ... cultures of Leptosphaeria maculans (A) and Alternaria brassicicola (B) The results are expressed as means and standard deviations of three independent experiments 7414 The purity of BHLmL2 obtained from ... 72% and 85% inhibition of BHLmL2 and BHAb, respectively, while mm 2-mer- captoethanol caused 85% and 97% inhibition of BHLmL2 and BHAb, respectively The influence of pH on the activities of the...
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Tài liệu Báo cáo khoa học: A knowledge-based potential function predicts the specificity and relative binding energy of RNA-binding proteins ppt

Tài liệu Báo cáo khoa học: A knowledge-based potential function predicts the specificity and relative binding energy of RNA-binding proteins ppt

... discrimination We anticipate that the performance of the potential will only increase with the size of the structural database and as terms are added to the model to account for protein and RNA intra-molecular ... structural data available for training Nevertheless, the distancedependent potential function already performs on par with the amber and rosetta force fields in decoy discrimination tests The impact of ... less favorable statistics (fewer structures of protein–RNA complexes) We anticipate that the increasing availability of protein– RNA structures, together with the availability of data on specificity, ...
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Tài liệu Báo cáo khoa học: Structural insights into the substrate specificity and activity of ervatamins, the papain-like cysteine proteases from a tropical plant, Ervatamia coronaria ppt

Tài liệu Báo cáo khoa học: Structural insights into the substrate specificity and activity of ervatamins, the papain-like cysteine proteases from a tropical plant, Ervatamia coronaria ppt

... N-benzoyl-Phe-Val-Arg-pNA D-Val-Leu-Lys-pNA D-Ile-Phe-Lys-pNA Ala-Ala-Val-Ala-pNA D-Ile-Pro-Arg-pNA Na-benzoyl-Arg-pNA D-Leu-Ser-Thr-Arg-pNA N-succinyl-Ala-Ala-Ala-pNA N-benzoyl-Val-Gly-Arg-pNA N-acetyl-Leu-Glu-His-Asp-pNA ... particular DNA codon for an amino acid at a particular position for this family of plant cysteine proteases The primers used were 5¢-TTGCCTGAGCATGTT GATTGGAGAGCGAAAG-3¢ (forward) and 5¢-GGGAT AATAAGGTAATCTAGTGATTCCAC-3¢ ... 8929–8936 Guha Thakurta P, Biswas S, Chakrabarti C, Sundd M, Jagannadham MV & Dattagupta JK (2004) Structural basis of the unusual stability and substrate specificity of ervatamin C, a plant cysteine...
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Tài liệu Báo cáo khoa học: The role in the substrate specificity and catalysis of residues forming the substrate aglycone-binding site of a b-glycosidase docx

Tài liệu Báo cáo khoa học: The role in the substrate specificity and catalysis of residues forming the substrate aglycone-binding site of a b-glycosidase docx

... Furthermore, these data may be of particular importance in understanding the physiological role of b-glycosidases and in designing inhibitors In addition, another important issue in understanding the aglycone ... details about the role of different residues of the aglycone-binding site in the stabilization of ESà and the interdependence between the binding of aglycone and the positioning of glycone in ... planned taking into account the residues found in the b-glycosidase from A B C D E Fig Comparison of the aglycone-binding site of some b-glycosidases (A) Aglycone-binding site of ZmGlu1 The aglycone...
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Tài liệu David prefers to live in the country rather than live in the city doc

Tài liệu David prefers to live in the country rather than live in the city doc

... tiết từ đó) David prefers to live in the country rather than in the city 2 Các bạn di chuột vào cụm từ để biết chức cụm câu: David prefers to live in the country rather than live in the city Tại ... *David prefers to live in the country rather than live in the city Hình thức ngữ pháp : Cấu trúc “ prefer to something rather than (do) something else” – ( thích làm việc ... prefer to do” “prefer doing” Nhưng có khác biệt cấu trúc: “prefer to something rather than (do) something else” cấu trúc “prefer doing something to (doing) something” – thích làm việc việc - in ...
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Báo cáo khoa học: Substrate specificity and transport mode of the proton-dependent amino acid transporter mPAT2 potx

Báo cáo khoa học: Substrate specificity and transport mode of the proton-dependent amino acid transporter mPAT2 potx

... transporter to the internal membrane side is the rate-limiting step and not – as proposed for most of the other electrogenic symporters – the return of the unloaded transporter to the outside of the membrane ... interpreted as the first evidence for a restricted velocity in the translocation step of the loaded transporter by the sterical conformation of the substrate The introduction of polar side groups in the ... properties of mPAT2 (Eur J Biochem 271) 3343 Table Apparent substrate affinities and transport currents of amino acids and derivatives as well as the corresponding inhibitory effect on the uptake of the...
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Báo cáo khoa học: Relation between domain evolution, specificity, and taxonomy of the a-amylase family members containing a C-terminal starch-binding domain pot

Báo cáo khoa học: Relation between domain evolution, specificity, and taxonomy of the a-amylase family members containing a C-terminal starch-binding domain pot

... involve also the cluster of acarviose transferase (Actsp), archaeal CGTase (Thcsp) and the maltogenic a- amylase (Bacst) due to its longer branch separating it from the Ó FEBS 2003 a- Amylase family members ... branches adjacent to each other and close to the border that separates the two major parts of the tree Note that the B stearothermophilus maltogenic a- amylase (Bacst) is placed in the ÔCGTase ... based on the alignments of the individual domains A, B, C and E (i.e the SBD) A tree was not constructed on the D domain because, as mentioned above, the fourdomain amylases and the two CGTases...
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Báo cáo khoa học: Hybrid reuteransucrase enzymes reveal regions important for glucosidic linkage specificity and the transglucosylation / hydrolysis ratio pptx

Báo cáo khoa học: Hybrid reuteransucrase enzymes reveal regions important for glucosidic linkage specificity and the transglucosylation / hydrolysis ratio pptx

... precisely the role of these two different regions and their amino acid residues in glucosidic linkage specificity The ratio of glucosidic linkages in the oligosaccharide and polymer products of these reuteransucrase ... also other regions affect the glucosidic linkage ratio within glucan and oligosaccharide products Results and Discussion The N-termini of the GTFA-dN and GTFO-dN reuteransucrases influence glucosidic ... catalytic domains of the GTFA and GTFO reuteransucrases are the main determinants for glucosidic linkage specificity Within these N-termini, the A1 ⁄ A2 and O1 ⁄ O2 parts of the reuteransucrase catalytic...
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Báo cáo khoa học: Kinetic studies and molecular modelling attribute a crucial role in the specificity and stereoselectivity of penicillin acylase to the pair ArgA145-ArgB263 pdf

Báo cáo khoa học: Kinetic studies and molecular modelling attribute a crucial role in the specificity and stereoselectivity of penicillin acylase to the pair ArgA145-ArgB263 pdf

... O atoms of the main chain CO groups of LeuB387 and TrpB240, Od1 atom of AsnB241, and Oc atom of SerB386 and is expected to stabilize the positive charge of ArgB263 All of these views remain arguable, ... substrates The data for the PA catalysed hydrolysis of PhAc-Asp and PhAc-Glu [13] and our data for PhAc-pAB, PhAc-mAB and PhAcoAB (Table 2) imply that the COOH group has to be positioned as in NIPAB ... penicillin acylase by AM1 calculations Amino acid residues of the enzyme are at their X-ray coordinates [19] Substrates having local symmetry of the leaving group (top left and bottom right) bind at...
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Báo cáo Y học: The role of hydrophobic active-site residues in substrate specificity and acyl transfer activity of penicillin acylase pdf

Báo cáo Y học: The role of hydrophobic active-site residues in substrate specificity and acyl transfer activity of penicillin acylase pdf

... phenylalanine was therefore mutated to Ala, Leu, Trp or Tyr These mutations may in uence the shape and volume of the acyl binding pocket and thereby alter the binding mode and affinity of the enzyme ... PAA and derivatives thereof, while maintaining the hydrophobicity of the binding site To test the effect of the mutations on the specificity for phenylacetylated substrates, the steady-state kinetic ... the deacylation reaction in the active site of the acyl- enzyme have been changed by the bF24A mutation The rate-limiting step in the synthesis reaction is the acylation of the enzyme and it therefore...
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Báo cáo khoa học: Modeled ligand-protein complexes elucidate the origin of substrate specificity and provide insight into catalytic mechanisms of phenylalanine hydroxylase and tyrosine hydroxylase pptx

Báo cáo khoa học: Modeled ligand-protein complexes elucidate the origin of substrate specificity and provide insight into catalytic mechanisms of phenylalanine hydroxylase and tyrosine hydroxylase pptx

... binding to the catalytic domains of PAH and TH Docking of the native cosubstrate BH4 into the crystal structure of the PAH catalytic domain yielded a total of 286 conformations Out of these, 44 ... PAH and TH has been used to model the full length structure of TPH [24] We have modeled the catalytic sites of PAH and TH and introduced BH4 and the amino acid substrates phenylalanine or tyrosine ... docking The computational approach allowed first, the creation of binary complexes of the natural pterin cosubstrate BH4 and the catalytic domain of the enzymes, followed by the docking of amino...
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