city and colour the thirst lyrics

... resulted in 55% and 51% loss of the initial activities of BHLmL2 and BHAb, respectively. These results strongly suggest that Ser is involved in the catalytic activity of BHLmL2 and BHAb and are consistent ... respectively. The influence of pH on the activities of the BH enzymes was investigated in the pH range 6–11. The pH optima were determined to be in the basic range (pH 8.0–10.0) for BHLmL2 and BHAb ... 6 and Fig. 4, the Hill equation provided an excellent fit, with coefficients of determination of 0.992 for BHLmL2 and 0.997 for BHAb. In addition, the fits of the V = f(S) curves were sigmoidal and...

Ngày tải lên: 18/02/2014, 14:20

... evaluated by the statistical potential used in the present study. In the case of the RNA muta- tions to the SRP complex, the mutated RNA residues are located in a double-stranded region of RNA, and do not ... onto the RNA bases nearest the center of the b-sheet structure of the RRM. We then scored these sequence-variant structures with the distance- dependent potential function. Figure 2 shows the ... score; the blue line represents the rank of these sequences using the distance-dependent potential. The points in each colored line are sorted independently by rank; the x-axis is the sort order. The...

Ngày tải lên: 18/02/2014, 16:20

... investigation of the catalytic mechanism and substrate speci city and to understand the differences therein for this class of enzymes. In order to identify the subsites of the ervatamins and to understand ... both the molecules of the asymmetric unit runs along the extended backbone of residues 65–64 and is partly exposed to the solvent (Fig. 2B). On the other hand, the P3 moiety of E-64 in each of the ... using the programs cns [58] and detwin and sfcheck in the CCP4 suite [59] confirmed that the data were twinned, and allowed us to calculate the twinning fraction (0.394) (Fig. 6A,B) and the twinning...

Ngày tải lên: 18/02/2014, 16:20

... binding of the Sfbgly interactions with the aglycone. Thus, the effect of the alteration of the glycone structure in the stabil- ity of the ES à complex (DDG à ) for the wild-type and mutant ... another important issue in understand- ing the aglycone speci city is the contribution of the interactions with the aglycone to the catalysis in b-glycosidases. Mutational studies with ZmGlu1 and SbDhr1 ... is that the interactions with aglycone do not affect the binding of the glycone within the Sfbgly active site. This hypothesis was further investigated by deter- mining the influence on the glycone...

Ngày tải lên: 18/02/2014, 17:20

... internal membrane side is the rate-limiting s tep and not – as proposed for most of the other electrogenic symporters – the return of the unloaded transporter to the outside of the membrane [17]. In ... alanine, serine and proline but also osmolytes such as sarcosine and betaine, and the D -enantiomers of serine and alanine. The apparent affinities of PAT1 substrates are mainly in the range of 2–15 ... The apparent K m values of D -Pro, D -Ala and OH-Pro increased only 1.5- and 2.1-fold by depolarizing the membrane from )120 to )20 mV. On the other hand, the affinity of b-Ala did not change significantly within...

Ngày tải lên: 07/03/2014, 16:20

... 3B). Thus for the SBD tree there is not an obvious border between the hydrolases and transglycosi- dases, but rather there may be one between the compact cluster of Bacillus and Thermoanaerobacter ... 251 CGTase with the SBDs from the CGTases from Thermococcus sp. B1001, Bacillus ohbensis,andThermoanaerobacter thermosulfurogenes are 37.3%, 63.8% and 74.0%, respectively. Even for the acarviose ... supports the idea that there has been a separate evolution of this domain [30]. This together with the findings of the present study indicates a separate evolution of the domains C and E compared to the...

Ngày tải lên: 08/03/2014, 08:20

... synthesis. Conclusions The N-termini of the catalytic domains of the GTFA and GTFO reuteransucrases are the main determinants for glucosidic linkage speci city. Within these N-ter- mini, the ... a) 8 barrel with the three catalytic resi- dues (Fig. 1C), determine the types and ratios of glu- cosidic linkages synthesized (Table 1). The A1 ⁄ O1 and A2 ⁄ O2 regions within the N-termini of the catalytic ... sequencing), both p15-GTFA-dN-RS and p15-GTFO-dN-RS were digested with XbaI and SalI, SalI and SacI, and SacI and KpnI, and corresponding fragments were exchanged, yielding the six constructs p15-GTFA-O1-dN-RS,...

Ngày tải lên: 16/03/2014, 04:20

... speci city is more pronounced and the synthetic capacity is, respectively, increased. In the latter system PA behaves as a typical transferase [14,15]. The nucleophile or S 1 ¢ [16] speci city of the ... close to the position found in the complex with PG [19]. The bond lengths and valence angles of the nitroaniline moiety were taken from the X-ray data for the system cyclophilin A and the tripeptide ... described [26]. The other substrates were obtained in dry organic solvents in the presence of organic base (pyridine, N-methylmorpholine). The solvents used for the synthesis of the respective...

Ngày tải lên: 16/03/2014, 16:20

... may influence the shape and volume of the acyl binding pocket and thereby alter the binding mode and affinity of the enzyme for PAA and derivatives thereof, while maintaining the hydrophobicity of the binding ... site. To test the effect of the mutations on the speci city for phenylacetylated substrates, the steady-state kinetic param- eters for the hydrolysis of the chromogenic substrate NIPAB and the inhibition ... interactions with PAA and shields the binding site from the solvent. A third phenylalanine, bF57, is located at the bottom of the hydrophobic cleft. The shortest distance between the side chain of bF57 and PAA...

Ngày tải lên: 17/03/2014, 23:20

... very similar, but the capacity of the organization in which they work and the tools of leadership and the resources on which they can draw diff er substantially.  ey also fi ll their positions ... delegator/organizer, the mayor helps the council and manager understand and maintain their roles, including helping the council members understand their responsibilities. Finally, in the policy initiator ... others to u nderstand and accept the leader’s point of view by the quality of the ideas presented and by the leader’s eff ectiveness at persuasion.  is approach does not depend on rewards and...

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