Site Of Unusual Clarity

... improve the femoral fixation of the spacer stem Alternatively to that and at stable femoral fixation, the spacer itself may dislocate out from the hip socket In these cases, specific attention ... fracture at the site of hip spacer implantation Right: Treatment consisted of spacer removal, and insertion of a cement-coated modular prosthesis with a spac...

Ngày tải lên: 26/10/2012, 09:53

... Furthermore, these data may be of particular importance in understanding the physiological role of b-glycosidases and in designing inhibitors In addition, another important issue in understanding the aglycone ... details about the role of different residues of the aglycone-binding site in the stabilization of ESà and the interdependence between t...

Ngày tải lên: 18/02/2014, 17:20

... E1aS283C and E1aF26 6A mutants behaved essentially the same as wild-type E1 In the DCPIP assay, the E1aY28 1A and E1aR28 2A mutants displayed a catalytic activity about twice that of wild-type E1 ... reaction mixture with E1 was incubated for exactly 10 at the relevant temperature and the catalytic activity at that temperature was then determined The inactivation...

Ngày tải lên: 20/02/2014, 23:20

... interaction of ATP with hGK, with possible implications for the positive kinetic cooperativity with respect to Glc Results ATP binding at active site of human glucokinase (WT) hGK and WT GST–hGK enzymes ... steady-state kinetic analyses are also compatible with conformational control of GK catalytic activity by the binding of (Mg )ATP, with...

Ngày tải lên: 06/03/2014, 00:20

... inhibitors of purified MAO -A, in agreement with the data of Ozaita et al (1997) for membrane-bound MAO -A and MAO-B [5] Spectral changes and the alteration of the redox properties of the flavin (Figs and ... for the active site than reported values for I2BS It seems clear that the active site of MAO is not the site to which I2BS ligands bind with nano...

Ngày tải lên: 07/03/2014, 10:20

... mutagenesis of aryl-alcohol oxidase model, site- directed mutagenesis and kinetic studies were used to identify the enzyme active site and evaluate the role of some selected residues in the catalytic ... investigated by site- directed mutagenesis The roles of the above aromatic and histidine residues are discussed below Moreover, the lower kcat and...

Ngày tải lên: 07/03/2014, 11:20

... residues are not described well and there is no example of mutational analysis of all of these residues in the same enzyme To obtain an insight into how the many conserved residues in the active sites ... circulans, whereas it may be mutated to asparagine without loss of activity in other family 18 chitinases [28,30] Preliminary results of a compara...

Ngày tải lên: 07/03/2014, 14:20

... LVCEETANYLVIWCIDLEVE 1A AAAAETANYLVIWCIDLEVE 2A LVCEAAAAYLVIWCIDLEVE 3A LVCEETANAAAAWCIDLEVE 4A LVCEETANYLVIAAAALEVE 5A LVCEETANYLVIWCIDAAAA N10 LVCEETANYL TANYLVIWCI M10 VIWCIDLEVE C10 B whole * IP: α-FLAG WB: ... immunoprecipitation with anti-FLAG and anti-Myc monoclonal antibodies (IP: a- FLAG and a- Myc), followed by immunoblotting with anti-FLAG and anti-Myc polyclonal antibodies...

Ngày tải lên: 07/03/2014, 21:20

... times were 2–5 The conformational changes induced in HDC by histidine and histidine- analogs involve alterations in the environment of the aromatic amino acid residues of the protein Fig Thermal denaturation ... decarboxylation and elimination of the fluoride, a reverse transaldimination can occur, so that an enamine form of the product can either leave th...

Ngày tải lên: 08/03/2014, 08:20

... C-terminal domain, including the highly conserved GSH -binding site (G -site) and the more divergent cosubstrate -binding domain or hydrophobic binding site (H -site) [7,18] The G -site includes both a-helices ... structure elements The topological arrangement of these elements is usually bababba, similar to the thioredoxin fold of other GSH -binding or cysteine -bin...

Ngày tải lên: 15/03/2014, 09:20

... charged a- amino group of SerB1, thus gaining additional stabilization The a- amino group of the aspartyl moiety in b-N-acetylglucosaminyl-l-asparagine has a similar function in the AGA-catalyzed ... [16], as are the calculations of Galabov et al concerning the energetics of the alkaline hydrolysis of N-phenylacetamides [19] The investigation of Perakyla et a...

Ngày tải lên: 16/03/2014, 01:20

... and catalytic properties of the mutants, and simulations of the substrate–enzyme interactions of the wild-type and one of the mutants The results provide indications of the native enzymes’ catalytic ... construction of the mutant b-glucosidases Findings that cytokinin-O-glucosides are natural substrates for both of the two b-glucosidases, Zm-p60.1 and Bgl4:1, b...

Ngày tải lên: 16/03/2014, 04:20

... observed with the A8B8 isoform of GAPDH leading to the A2B2 isoform with a higher NADPHdependent activity [39] By analogy with the CTE of the isoform of GAPDH [15,37], we hypothesize that the disulfide ... Involvement of the negative charge of the residue D36 of CP12 in the interaction between CP12 and GAPDH In order to confirm that residues from the...

Ngày tải lên: 16/03/2014, 13:20

... presence of < /b> stigmatellin, which binds in < /b> the < /b> distal lobe of < /b> the < /b> Qo < /b> site,< /b> and myxothiazol, azoxystrobin and pyraclostrobin, which bind at the < /b> proximal lobe of < /b> the < /b> Qo < /b> site < /b> (Fig and Table 1) The < /b> control ... no growth Growth on Strains AB4 AB13 AB5 AB18 AB7 AB8 AB9 AB10 AB16 AB11 AB12 Myxothiazol 10 Stigmate...

Ngày tải lên: 16/03/2014, 16:20

... of phosphorylation rate seen at the concentration x of H4biopterin vo is the rate in the absence of H4biopterin and vmin is the rate at very high concentrations of H4biopterin The observed Hill ... H4biopterin, the oxidized cofactor H2biopterin and the catecholamine inhibitor dopamine From Fig 3A it is seen that on preincubation with saturating concentratio...

Ngày tải lên: 17/03/2014, 09:20