... analysis of bcp1, bcp3 and bcp4 under oxidative stress The involvement of bcp1, bcp3 and bcp4 in oxidative stress was investigated by assessing mRNA levels following treatment of S solfataricus ... contrast, the Bcp3 sequence only shows one cysteine at position 42 in the N-terminal region Expression and purification of Bcp1, Bcp3 and Bcp4 bcp1, bcp3 and bcp4 were amplified by PCR from S solfataricus ... form of pUC19 are indicated on the left by arrows Temperature dependence and thermostability of Bcp1, Bcp3 and Bcp4 To characterize the thermophilicity of recombinant Bcp1, Bcp3 and Bcp4, we investigated...