Intein mediated generation of n terminal cysteine proteins and their applications in live cell bioimaging and protein microarray

... shown) Inhibition of pneumococcal murein hydrolases by bicyclic amines As shown above, tropic esters of bicyclic amines were selected and characterized by biophysical methods as strong ligands ... that the bicyclic amines are also able to bind to the active site of Pce Effect of choline analogs on cell growth and viability Fig Effect of choline and an...

Ngày tải lên: 19/02/2014, 05:20

... information, the protein ligand interaction can be probed by [15N]-HSQC FEBS Journal 273 (2006) 4154 – 4159 ª 2006 The Authors Journal compilation ª 2006 FEBS 4157 15 N-labelled proteins by cell-free ... accelerated studies of proteins by NMR spectroscopy by assigning residue type information to every amide cross-peak observed in [15N]-HSQC spectra We anticipate that...

Ngày tải lên: 07/03/2014, 12:20

... artificially introduced cross-links in proteins Cross-links impose distance constraints on amino acid residues that can be used to model the 3-D structure of proteins and protein complexes [3–7] Mass spectrometric ... structures, chemical modifications and cross-linking in protein mixtures, complexes, and assemblies Proteins A single protein, a list of p...

Ngày tải lên: 07/03/2014, 12:20

... the cuticular extract Purification of chitin-binding proteins Fig TGase-dependent protein cross-linking of cuticular chitinbinding proteins Lane 1, nontreated cuticular proteins; lane 2, cuticular ... Y9), and repeats of the di- and tri-peptide sequences QQ and QQQ were observed in the amino terminal sequences of basic QH proteins and 10 Nucleotide sequences...

Ngày tải lên: 07/03/2014, 21:20

... six cysteines in yeast Erv1p was the major goal of this paper All three pairs of cysteines in Erv1p are indispensable for in vivo activity The six cysteines of yeast Erv1p can be grouped into ... any activity in vitro or in vivo This phenotype is in agreement with the finding that the C130–C133 pair is part of the primary redox- acti...

Ngày tải lên: 17/03/2014, 10:20

... binding of NCp7 to the C-terminal domain (80–96 )Vpr [8] may expose the N-terminal (1–39) portion of the protein and allow its tight binding to Lys-tRNA synthetase [15] The C-terminal domain (52–96 )Vpr ... when comparing the isolated domains of (1–51 )Vpr [25] and (52–96 )Vpr [26] with the intact Vpr protein The differences in structure are loc...

Ngày tải lên: 31/03/2014, 23:20

... N-terminal and a Cterminal region of CIITA interacting with the viral transactivator, although, as stated above, only the Nterminal region is involved in the inhibition of Tax-2 function Interestingly, ... the replication of the human HTLV-2 retrovirus by inhibiting the function of the viral transactivator protein Tax-2 [21,22] However the bioche...

Ngày tải lên: 18/06/2014, 22:20

... (Figure 1A) This finding is in keeping with the findings of NS1/NS2 antagonism of type I IFNs [4,46,47] and suggests the possibility that type I IFN antagonism is linked to NS1/NS2 induction of ... interfere with direct TLR signaling, but instead regulate paracrine IFN signaling [7] The SOCS protein family is comprised of eight proteins (CIS, cytokine- induci...

Ngày tải lên: 20/06/2014, 01:20

... recognises the viral receptor, and TM, the transmembrane protein, carries the fusion activity and tethers the GP to virions [4-6] However, retroviruses and lentiviruses can be pseudotyped by a number of ... contain an scFv (αMHC and αHEL, [27]), upstream of the GP gene Consequently, the resulting constructs (αMHC /VSV-G and αHEL /VSV-G) express the gene from the MLV L...

Ngày tải lên: 20/06/2014, 01:20

... Efficacy of biotinylation was assessed with the 4hydroxyazobenzene-2-carboxylic acid (HABA) assay To ensure that the affinity of the antibodies for the receptors was not compromised in any way after ... frequency was determined with the Hardy–Weinburg formula Results Surface expression of Fcγ receptors on peripheral leucocytes Expression of FcγRIII Analysis of receptor expressio...

Ngày tải lên: 09/08/2014, 10:20

... normal as an indicator for intravascular volume loss and therefore as an initial marker of bleeding Stewart and colleagues [4] recently analyzed BNP and transthoracic echocardiogram in trauma patients ... MOF: an early-onset MOF (EMOF) occurring on days to after trauma, and a late-onset MOF (LMOF) starting days after trauma In this context, Maier and colleagues [1] differed...

Ngày tải lên: 13/08/2014, 11:22

... outcome of critically ill patients [13] It has been estimated that as many as 15% to 30% of ICU admissions are complicated by some degree of myocardial injury and that as many as 40% of patients ... had full access to all of the data in the study and takes responsibility for the integrity of the data and the accuracy of the data analysis J-PQ participated in study concep...

Ngày tải lên: 13/08/2014, 11:23

... http://ccforum.com/content/15/1/R42 Page of 36 Harada E, Nakagawa O, Yoshimura M, Harada M, Nakagawa M, Mizuno Y, Shimasaki Y, Nakayama M, Yasue H, Kuwahara K, Saito Y, Nakao K: Effect of interleukin-1 beta on cardiac hypertrophy ... N-terminal pro-brain natriuretic peptide and C-reactive protein to predict ICU mortality in unselected medical ICU patients...

Ngày tải lên: 14/08/2014, 07:21

... Three intein- mediated protein biotinylation strategies: (A) in vivo biotinylation in live cells; (B) in vitro biotinylation of column-bound proteins; & (C) cell-free biotinylation of proteins 10 ... (Lys239) -intein 14 2.4 Expression of intein- fused proteins 14 2.5 Affinity purification & C-terminal biotinylation of recombinant proteins 15 2.6 SPR analy...

Ngày tải lên: 08/11/2015, 16:30

... Inteins and protein splicing Inteins and their protein splicing abilities are becoming increasing invaluable tools in protein engineering.[1] Protein splicing is a cellular processing event that ... Protein N H NH2 ASN O O HS CYS Intein NH + H 2N Protein ASN O Figure Mechanism of intein splicing at the C -terminal junction of the Ssp DnaB intein, with Asn as t...

Ngày tải lên: 08/11/2015, 16:30