... to 15 lm for P50H, H50P, P128H, H128P, PigE17DCPT1B and HumanD17ECPT1B, and from to 500 lm for D18PigCPT1B and D28PigCPT1B) The assay was performed at mm carnitine as standard To analyze PigE17DCPT1B ... is responsible for the peculiar characteristics of the pig enzyme (low carnitine Km and high malonylCoA IC50) and, whereas Glu17 acts as a negative determinant for malonyl-CoA sensitivity in pig ... determinant for malonyl-CoA sensitivity of the human enzyme, and showed that the variant, Glu17, in the pig enzyme is responsible for its peculiar kinetic characteristics (low carnitne Km and high malonyl-CoA...