Generation of aptamers against the ganglioside binding proteins via single bead selex

... costs of proteins such as the gsbp Generation of aptamers against the ganglioside- binding proteins via single bead SELEX 18 Chapter Experimental Figure Representation of the single bead SELEX ... successfully in the course of this project by using the 19mer ganglisoside binding peptide from the amino acid Generation of aptamers against...

Ngày tải lên: 07/10/2015, 09:55

... Guo H, He Y, Zhou Y, Zheng BJ, Jiang S: Recombinant receptor-binding domain of SARS-CoV spike protein expressed in mammalian, insect and E coli cells elicits potent neutralizing antibody and protective ... showed that the spike RBD could induce potent and persistent antibody responses in the recovered patients (Figure and 2); with a RBD-Fc fusion...

Ngày tải lên: 12/08/2014, 02:20

... effect of the double mutant on the basal level of transcription in EtOH-treated controls We then used this background of the blocked activation pathway to test whether activation by methyl epoxyfarnesoate ... dependent on the increasing dose of the added wildtype dUSP Again, over the range of the monotonic restoration of methyl epoxyfarnesoate-activated transc...

Ngày tải lên: 23/03/2014, 21:20

... Regulation of complement activation by C-reactive protein: targeting of the inhibitory activity of C4b -binding protein J Immunol 2006, 176:7612-7620 Webb JH, Blom AM, Dahlback B: The binding of protein ... modulation of factor H and C4 bindingprotein synthesis in human monocytes Biochem J 1990, 271:767-772 Barnum SR, Dahlback B: C4b -binding protein, a regulator...

Ngày tải lên: 09/08/2014, 10:21

... Defining potentially conserved RNA regulons of homologous zinc-finger RNA- binding proteins Genome Biology 2011 12:R3 Submit your next manuscript to BioMed Central and take full advantage of: • Convenient ... intron of ZNF9 cause myotonic dystrophy type (DM2) DM2 is characterized by heterogeneous, multi-systemic symptoms, including myotonia [29] The disease is thought to be...

Ngày tải lên: 09/08/2014, 22:23

... in- depth analysis of the case in point, Batam, specifically on, BatamIndo Industrial Park Chapter outlines the profile of factory workers in the manufacturing sector, who are mostly young, single ... industrialization is the feminization of its workforce A profile of female factory workers in Batam and the environment and social landscape of Batamindo...

Ngày tải lên: 06/10/2015, 21:15

... or NMR structural analyses and the construction of artificial libraries allow constructive selection and functionalization not only of antibody fragments, but also of small, nonantibody proteins ... demonstrating the utility of the grafting of functional peptides with randomized edge sequences for optimizing the orientation of the grafted peptide on a scaffold p...

Ngày tải lên: 06/03/2014, 11:20

... discrimination We anticipate that the performance of the potential will only increase with the size of the structural database and as terms are added to the model to account for protein and RNA intra-molecular ... structural data available for training Nevertheless, the distancedependent potential function already performs on par with the amber and rosetta force...

Ngày tải lên: 18/02/2014, 16:20

... FnBPA-2, FnBPA-3, FnBPA-4, FnBPA-5, FnBPA-6, FnBPA-7, FnBPA-8, FnBPA-9, FnBPA-10, and < /b> FnBPA-11, and < /b> FnBPB-1, FnBPB-2 ⁄ 3, FnBPB-4, FnBPB-5, FnBPB-6, FnBPB-7, FnBPB-8, FnBPB-9, FnBPB-10, and < /b> FnBPB-11, ... Fn ABP Fn ABP Fn ABP Fn ABP Fn A-< /b> 8 B Fn PA BP -9 Fn ABP 10 A < /b> -1 0.0 Fig Binding of < /b> Fn to the < /b> predicted FnBRs of < /b> FnBPB and < /b> FnBP...

Ngày tải lên: 06/03/2014, 22:21

... 2675 Actin and ABPs in transcription regulation B Zheng et al Table Role of nuclear actin- binding proteins interacting with the androgen receptor AR, androgen receptor; LBD, ligand-binding domain ... STARS-interacting proteins These novel proteins contain four LIM domains and a C-terminal villin headpiece domain, which mediates actin- binding in several proteins,...

Ngày tải lên: 07/03/2014, 01:20

... important signaling role of the ICDs released by the APP family of proteins, but may impact on other functions of this family of proteins Experimental procedures Reagents Unless otherwise specified, chemicals ... gamma-Secretase cleavage and binding to FE65 regulate the nuclear translocation of the intracellular C-terminal domain (ICD) of the APP family...

Ngày tải lên: 15/03/2014, 10:20

... dissociation constant The rate of clearance of the ternary T–AT–Fab complex in rats was only slightly slower than the rate of clearance of T–AT Studies with cultured HepG2 cells indicated that M27 ... stoichiometry of binding In vivo clearance of the T–AT complex in rats Thrombin, native AT, and elastase-cleaved AT were labeled with 125I (Amersham Pharmacia A...

Ngày tải lên: 17/03/2014, 10:20

... supported by the 2678 structurally rigid b-sandwich framework of the paired variable domains of the light and heavy chains These CDRs come together at the tips of the Y-shaped molecule to form a ... buried in the hydrophobic interior of lipocalins – but rather serve Anticalins for cross-linking the N- and C-terminus to the b-barrel [5] – they are not as c...

Ngày tải lên: 23/03/2014, 07:20

... with the molting hormonal activity (pEC50), indicating that the strength of the hormonal activity of ecdysone agonists is primarily determined at the step of their binding to the EcR–USP complex ... is useful for binding assays of ecdysone agonists The binding activity of a number of steroidal and nonsteroidal ecdysone agonists was...

Ngày tải lên: 23/03/2014, 21:20

... an increase in the gene expression of SREBPs, and second, the enhancement of SREBP processing by glutamine We therefore investigated the effect of glutamine on the gene expression of SREBP family ... increased, but then the mRNA levels of both SREBPs and their target genes became significantly elevated at and 12 h (Fig 4) Based on the fact that...

Ngày tải lên: 28/03/2014, 22:21