Chemical proteomics approaches to study aspartic and metalloproteases

... ligands with potential for derivitizing into therapeutic agents Herein, we aim to expand the scope of chemical proteomics through the development of two novel small molecule-based approaches towards ... Profiling of Aspartic and Metalloproteases 2.1 Affinity-based Proteomic Profiling of Metalloproteases 16 2.1.1 Design of Photoactivable Affinity-based Probes for 16 Metallopro...

Ngày tải lên: 03/10/2015, 20:33

... either: (a) dsRNA; or (b) small interference (si )RNA The longer dsRNA may generate a large population of siRNA (with 21–23 nucleotides), and the use of longer dsRNA may be advantageous over siRNA ... corresponding to the mature peptide of MeMIH-B was amplified by PCR using T7 promoter-linked primers (forward, 5¢-TAATACGACTCACTATAGGTACTATG TATCGCATGCCAAT-3¢; reverse, 5¢-...

Ngày tải lên: 16/03/2014, 05:20

... visualizing my hands moving through the numbers, one adding and one subtracting Teach It - Find someone who doesn’t understand the topic and teach it to them This exercise forces you to organize ... Reading lots and having a general understanding of many topics gives you a lot more flexibility in finding patterns and metaphors in new topics The more you already know, the easier it...

Ngày tải lên: 18/10/2013, 12:15

... Intermediate level at FLC-HPU?  What is the main reasons of the difficulties in vocabulary teaching and learning at Pre – Intermediate level at FLC-HPU?  Do games help learners at Pre – Intermediate ... language teaching and their relevance to vocabulary 1.4 Recent research about teaching and learning second language vocabul...

Ngày tải lên: 29/01/2014, 14:36

... understand what makes proteins ÔtickÕ and to engineer proteins with native-like properties In this review we discuss combinatorial approaches toward understanding protein structure and stability ... Systematic and de novo methods of protein design and redesign have been excellently reviewed elsewhere, and we will focus here on combinatorial methods [25–31] Selecting...

Ngày tải lên: 19/02/2014, 12:20

... 1.1 Introduction The Board of Directors of the Reserve Bank of India, at its meeting held on October 15, 2010 formed a Sub-Committee of the Board to study issues and concerns in the microfinance ... assets of the banking system, it is increasing rapidly 3.7 Finally, given the need to encourage the growth of the Microfinance sector and...

Ngày tải lên: 15/03/2014, 14:20

... THEORETICAL AND METHODOLOGICAL APPROACHES TO SOCIAL SCIENCES AND KNOWLEDGE MANAGEMENT Edited by Asunción López-Varela Theoretical and Methodological Approaches to Social Sciences and Knowledge ... historical, philosophical, and theoretical developments that have influenced social research methods worldwide 4 Theoretical and Methodological Ap...

Ngày tải lên: 29/06/2014, 09:21

... Figure Planar cell polarity (a) Epithelial tissues display apical-basal and planar polarity Hair structures are generated at the apical (top) surface and are absent from the basal (bottom) surface, ... and the downstream effectors that need to be mobilized to generate specific properties of tissue organization Can defects in planar polarity cause human disease? Planar...

Ngày tải lên: 06/08/2014, 19:21

... describe the design and synthesis of OrlistatTM -like natural product -based probes (Chapter 2, & 4), K11777 -like drug candidate -based probes (Chapter 5) and azanitrile-containing small molecules (Chapter ... 1.2 Activity- Based Protein Profiling (ABPP) 1.2.1 Introduction 1.2.2 Activity- Based Probe Design 1.2.3 Bioorthogonal Chemistry in ABPP 10 1.2.4 Application...

Ngày tải lên: 09/09/2015, 18:54

... Stock, and Stoker, 1989; H¨dle and Stoker, 1989; Ichimura, 1993; Klein and Spady, 1993; H¨rdle, Hall, and a a Ichimura, 1993; Sherman, 1994; Horowitz and H¨rdle, 1996; Hristache, Juditsa ki, and ... SOME APPROACHES TO NONLINEAR MODELING AND PREDICTION WANG TIANHAO (B.Sc East China Normal University) A THESIS SUBMITTED FOR THE DEGREE OF DOCTOR OF PHILOSOPHY DEPARTMENT O...

Ngày tải lên: 10/09/2015, 09:24

... of using zebrafish as a liver model 15 1.4.2 Study of liver development in zebrafish 16 1.4.2.1 Liver organogenesis in zebrafish 16 1.4.2.2 Study liver development through genetic ... genetic studies of liver organogenesis 1.4.2 Study of liver development in zebrafish Study of liver development in zebrafish has a relatively short history Cur...

Ngày tải lên: 14/09/2015, 12:11

... NOVEL LIPIDOMIC APPROACHES TO ANALYSE GLYCEROPHOSPHOLIPIDS AND SPHINGOLIPIDS IN COMPLEX MIXTURES USING MASS SPECTROMETRY GUAN XUE LI (B.Sc (Hons.), National University of Singapore) ... their interacting factors In this thesis, I describe the development of a novel mass spectrometry- based approach for comprehensive profiling of glycerophospholipids and...

Ngày tải lên: 14/09/2015, 14:06