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CHEMICAL AND BIOLOGICAL CHARACTERIZATION OF LIGUSTICUM WALLICHII EXTRACT IN 3t3 l1 AND HEP g2 CELL LINES

CHEMICAL AND BIOLOGICAL CHARACTERIZATION OF LIGUSTICUM WALLICHII EXTRACT IN 3t3 l1 AND HEP g2 CELL LINES

CHEMICAL AND BIOLOGICAL CHARACTERIZATION OF LIGUSTICUM WALLICHII EXTRACT IN 3t3 l1 AND HEP g2 CELL LINES

... EFFECTS OF PHTHALIDES ON 3T3- L1 CELL LINE Hypothesis Phthalides extracts from chuangxiong influence preadipocyte proliferation and differentiation in 3T3- L1 murine cell model Introduction According ... duplication and cell division The increase of sub-G1 phase from cell cycle analysis showed that IPE had a minor or insignificant influence on apoptosis induction in Hep- G2 cells In contrast, ... via interfering cell cycle development but the underlying mechanisms remain unknown In the current findings, the growth of Hep- G2 cells was inhibited after treated with IPE Furthermore, the inhibition...
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Báo cáo khoa học: Antioxidant defences and homeostasis of reactive oxygen species in different human mitochondrial DNA-depleted cell lines pot

Báo cáo khoa học: Antioxidant defences and homeostasis of reactive oxygen species in different human mitochondrial DNA-depleted cell lines pot

... (Figs 3–5) Antioxidant defences of q0 cell lines GSH-GSSG and GR We measured GSH and GSSG in exponentially growing cells, as GSH content changes in the growth and lag phases [51] In all q0 cells studied, ... cells, and that there are cell line-to -cell line variations in intracellular antioxidant defences and ROS homeostasis In fact among the studied cells, those originating from bone are particularly ... Determination of intracellular reactive oxygen species as function of cell density Methods Enzymol 352, 91–100 44 Zuo, L & Clanton, T.L (2002) Detection of reactive oxygen and nitrogen species in...
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CHEMICAL AND BIOLOGICAL CHARACTERIZATION OF TRACE AMOUNT OF SEX HORMONE RECEPTOR ACTIVE COMPOUNDS IN PROSTATE CANCER RELATED BIOLOGICAL SAMPLES

CHEMICAL AND BIOLOGICAL CHARACTERIZATION OF TRACE AMOUNT OF SEX HORMONE RECEPTOR ACTIVE COMPOUNDS IN PROSTATE CANCER RELATED BIOLOGICAL SAMPLES

... Validation of A4 in g/L of PBS/BSA Table 5a Interday Validation of T in g/L of PBS/BSA Table 5b Intraday Validation of T in g/L of PBS/BSA Table 6a Interday Validation of DHT in g/L of PBS/BSA ... 4.3.2 Determination of Saturating Doses of Known AKR1C3 Inhibitor and Dimethoxycurcumin and its Potential as AKR1C3 Enzyme Inhibitor 126 4.3.3 Investigation of Dimethoxycurcumin using LC-MS/MS ... Mechanisms Behind Development of Castration Resistant Prostate Cancer Table Analysis of LC-MS/MS Pros and Cons in Clinical Diagnostics Table 4a Interday Validation of A4 in g/L of PBS/BSA Table 4b Intraday...
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Tài liệu Báo cáo khoa học: Neuronal growth-inhibitory factor (metallothionein-3): evaluation of the biological function of growth-inhibitory factor in the injured and neurodegenerative brain pdf

Tài liệu Báo cáo khoa học: Neuronal growth-inhibitory factor (metallothionein-3): evaluation of the biological function of growth-inhibitory factor in the injured and neurodegenerative brain pdf

... discovery of a brain- specific MT isoform, GIF, in 1991, sparked considerable interest in understanding the role of all MTs in the brain, and particularly within the injured or diseased brain In the ... studies examining the role of GIF in AD, linked to its initial discovery as a factor deficient in the AD brain There remains no clear consensus on the role of GIF in the pathogenesis of AD, and therefore ... FEBS 2935 Function of GIF in injured and degenerative brain C Howells et al transection of mature neurons [31] However, in the absence of brain extract (either AD or from the normal brain) GIF...
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Tài liệu Báo cáo khoa học: Characterization of electrogenic bromosulfophthalein transport in carnation petal microsomes and its inhibition by antibodies against bilitranslocase docx

Tài liệu Báo cáo khoa học: Characterization of electrogenic bromosulfophthalein transport in carnation petal microsomes and its inhibition by antibodies against bilitranslocase docx

... mgÆmL)1 BSA and stored at )20 °C Electrogenic BSP uptake inhibition by antibodies The kinetics of bilitranslocase transport activity inhibition by antibodies were examined by preincubating 24 lL ... addition of protein (2.6 ± 0.04 lmolÆmin)1Æmg protein)1, with lg valinomycin), as well as of K+ (6.25 ± 0.16 unitsÆmEq)1 K+, with lg valinomycin) and valinomycin (0.51 ± 0.03 unitsÆlg)1 valinomycin, ... also investigated in carnation petal microsomes by preincubating them with antibody A in the presence of increasing concentrations of bilirubin BSP uptake was assayed to track the progress of the...
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Báo cáo khoa học: Purification and characterization of the cysteine proteinases in the latex of Vasconcellea spp. ppt

Báo cáo khoa học: Purification and characterization of the cysteine proteinases in the latex of Vasconcellea spp. ppt

... in the higher activity observed in Vasconcellea spp.: (1) a higher protein content in their latex; and (2) the presence of other cysteine proteinases or isoforms in the latex To investigate these ... relationships of cysteine proteinases it can be deduced that the common ancestor of the genera Carica and Vasconcellea already contained at least two different cysteine proteinases in its latex (papain and ... cysteine proteinases of C papaya and V cundinamarcensis The cysteine proteinases of V · heilbornii hold the generally conserved P2, Q19 and the 11GAVTP15-motif located at the N-terminus of papain-like...
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Báo cáo khoa học: Identification and characterization of novel PKA holoenzymes in human T lymphocytes pdf

Báo cáo khoa học: Identification and characterization of novel PKA holoenzymes in human T lymphocytes pdf

... than the activity in the precipitate from the Ca1 transfected cells demonstrating that the Cb2 antibody in combination with PKA kinase assay could be used to detect specifically Cb2 activity To ... PKA- specific kinase activity, as shown in Fig 4B This demonstrated that precipitates extracted with 8-CPTcAMP contained cAMP-inducible PKA activity that could be inhibited by protein kinase inhibitor (PKI) ... 8-CPT-cAMP were detected in both immunoprecipitates implying that Ca1 and Cb2 are both associated with RIa and RIIa in human T cells This 1563 Novel PKA holoenzymes in human T lymphocytes S Ørstavik...
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Báo cáo khoa học: Identification and functional characterization of an aggregation domain in long myosin light chain kinase ppt

Báo cáo khoa học: Identification and functional characterization of an aggregation domain in long myosin light chain kinase ppt

... Aggregation domain in myosin light chain kinase A Fig Prediction for a conserved aggregation domain in the 4Ig region of L-MLCK and recombinant expression of MLCK variants (A) The sequences of ... necrosis factor-induced long myosin light chain kinase transcription is regulated by differentiation-dependent signaling events: characterization of the human long myosin light chain kinase promoter ... J & Zhi G (1998) Myosin light chain kinase: functional domains and structural motifs Acta Physiol Scand 164, 471–482 Kamm KE & Stull JT (2000) Dedicated myosin light chain kinases with diverse...
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Báo cáo sinh học:

Báo cáo sinh học: " Genetic characterization of measles viruses isolated in Turkey during 2000 and 2001" pdf

... showing province and number of measles virus isolates obtained during 2000- 2001 Figure virus isolates obtained during 2000 2001 Map of Turkey showing province and number of measles Map of Turkey ... COBL COBL COBL urine urine urine urine urine urine urine throat swab urine urine throat swab nasal swab urine urine urine urine throat swab urine urine urine blood blood urine urine sporadic sporadic ... Phylogenetic analysis of the N gene sequences of wild-type MVs isolated in Turkey Phylogenetic analysis of the N gene sequences of wild-type MVs isolated in Turkey Sequences of the Turkish viruses...
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báo cáo hóa học:

báo cáo hóa học:" Genetic characterization of measles viruses isolated in Turkey during 2000 and 2001" potx

... showing province and number of measles virus isolates obtained during 2000- 2001 Figure virus isolates obtained during 2000 2001 Map of Turkey showing province and number of measles Map of Turkey ... COBL COBL COBL urine urine urine urine urine urine urine throat swab urine urine throat swab nasal swab urine urine urine urine throat swab urine urine urine blood blood urine urine sporadic sporadic ... Phylogenetic analysis of the N gene sequences of wild-type MVs isolated in Turkey Phylogenetic analysis of the N gene sequences of wild-type MVs isolated in Turkey Sequences of the Turkish viruses...
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Báo cáo hóa học:

Báo cáo hóa học: " Synthesis and Characterization of Monodispersed Copper Colloids in Polar Solvents" pdf

... coordination bonds Conclusions In this letter, two monodispersed copper colloids in water and E.G have been prepared In order to obtain pure-phase copper colloid in water, the reaction time of ... structure of a polyvinyl skeleton with nitrogen and oxygen polar groups, and the polar group donates lone-pair electrons forming a coordinative interaction with copper ions, thus creating the Cu2?–PVP ... reaction rate, the amount of reducing agent and reaction mechanism were discussed The roles of polyvinyl pyrrolidone (PVP) and ascorbic acid were investigated Characterization of Copper Nanoparticles...
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Báo cáo y học:

Báo cáo y học: "Early Characterization of Toll-like receptors in primary lung epithelial cells: strong impact of the TLR3 ligand poly(I:C) on the regulation of Toll-like receptors, adaptor proteins and inflammatory response" ppt

... is in accordance with the highly increased cytokine and chemokine secretion induced by poly(I:C) and emphasizes the strong proinflammatory impact of the TLR3 ligand poly(I:C) for SAEC Regulation ... expression of other TLRs and proteins involved in TLR signaling in SAEC In addition, we demonstrate that the expression of TLRs and their signaling proteins in SAEC is strongly regulated by type-1 and ... release of inflammatory mediators The family of Toll-like receptors (TLR) plays a key role in pathogen recognition and induction and regulation of the innate and adaptive immune response Airway epithelial...
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báo cáo khoa học:

báo cáo khoa học: " Exploiting EST databases for the development and characterization of EST-SSR markers in castor bean (Ricinus communis L.)" pptx

... frequency and distribution of SSRs in castor bean ESTs, (2) the establishment and validation of EST- SSR markers for detection of polymorphism in castor bean, and (3) the assessment of genetic ... an increasing need for distinguishing the varieties reliably, establishing their purity, and fingerprinting released varieties, hybrids and the parental lines of castor bean germplasm held in ... doubt, the polymorphic EST- SSR markers developed herein will provide robust genetic markers for further investigation of the origin and evolution of castor bean, though the geographic structuring of...
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Báo cáo y học:

Báo cáo y học: "Characterization of monocyte/macrophage subsets in the skin and peripheral blood derived from patients with systemic sclerosis" pps

... Immunoperoxidase staining of skin in SSc patients compared with that in healthy controls Skin sections from (a to c) healthy controls and (d to f) systemic sclerosis (SSc) patients were stained with anti-human-CD68, ... no link between CD163+ or CD204+ monocyte/macrophage lineage and SSc has been established in the skin or in the peripheral blood of SSc patients Therefore, to more fully explore the nature of ... cells in circulating monocytes of SSc patients by flow cytometric analysis We suggest that CD163+ or CD204+ macrophages in the skin and the CD14brightCD163+CD204+ monocyte subset in the peripheral...
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Báo cáo y học:

Báo cáo y học: "Phenotypical and functional characterization of alveolar macrophage subpopulations in the lungs of NO2-exposed rats" pot

... subpopulations in NO2-exposed animals The occurrence of phenotypically different AM subpopulations may either be explained by a functional shift of already present AM or by the infiltration of macrophages ... [39,40] In conclusion, our findings clearly suggest that the newly recruited ED7+ AM are involved in the regulation of the ongoing inflammatory process Whether the antiinflammatory effects of IL10 ... Germany) The number of living cells was determined using the CASY®1 Cell Counting System (Schärfe Systems, Reutlingen, Germany) and AMs were incubated at a final concentration of × 106 cells/ml in...
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