Characterization of major and unique blomia tropicalis mite allergens

... mite allergens 2.3 Domestic mites Domestic mites consist of various free-living mites that are found living in houses This includes house dust mites (HDM) (family Pyroglyphidae) and storage mites ... 56 v Cloning of a unique allergen from Blomia tropicalis and monoclonal antibody production 58 4.1 Objectives and significance of the study 58 4.2 Materials and methods .....

Ngày tải lên: 03/10/2015, 20:32

... Superimposition of the NMR structure of Blo t 21 with Blo t and Der p 58 Figure 3 .7 The allergenicity of Blo t 21, Der f 21, Der p and Blo t 60 Figure 3.8 The CD spectrum of Blo t 21, Der f 21, Blo t and ... cells 25 Blot21_E74A_up Blot21_E74A_down Blot21_K76A_down Blot21_K76A_down Blot21_D79A_up Blot21_D79A_down Blot21_E84A_up Blot21_...

Ngày tải lên: 09/09/2015, 18:55

... (Ramb-Lindhauer C et al., 1991; van Reijsen FC et al., 1992) Animal Dander and Cockroach Allergens Animal dander and cockroach allergens have been studied in association with asthma and allergic ... airway hyperresponsiveness, airway inflammation and airway remodeling, features of acute and chronic asthma that resembled human asthma, and these pathological changes cou...

Ngày tải lên: 12/09/2015, 10:54

... months time [10 2] Acute cortical damage to auditory processing structures might be assessed objectively in a complementary manner, via studies of the MMN [10 3] 15 2 10 11 12 13 14 15 16 17 18 Closing ... Shappell SA, Brandt ME Psychophysiology of N200/ N400: A review and classification scheme Advances in Psychophysiology 19 91; 4:43 -10 6 Hoffman JE Event-related pot...

Ngày tải lên: 02/11/2012, 11:08

... desolvation of the phosphate group Nevertheless, although the binding enthalpy of the inhibitors is negative, the binding enthalpy of 6PG is Table Binding parameters of substrate and substrate ... A binding isotherm and the fitted data for the binding of aPyADP to the enzyme are shown in Fig The binding stoichiometry was close to two Table Binding parameters...

Ngày tải lên: 07/03/2014, 05:20

... Divergences of rat alkaline phosphatase isozymes T Harada et al A B Fig Stereoviews of the residues near metal-binding site in (A) rat intestinal alkaline phosphatase- I (rIAP-I) and (B) rIAP-II ... Residues indirectly associating with the metal via water molecules Fig Immunological detection of rat intestinal alkaline phosphatase (rIAP) isozymes in rat...

Ngày tải lên: 07/03/2014, 17:20

... Molecular basis of photoprotection and control of photosynthetic light- harvesting Nature 436, 13 4–1 37 18 Horton P & Ruban A (2005) Molecular design of the photosystem II light- harvesting antenna: photosynthesis ... absorption at 680 nm (blue–green light) and 700 nm (red and far-red light) , respectively State is induced by excess PSI light (light 1) and State...

Ngày tải lên: 16/03/2014, 06:20

... the identification and characterization of solanesyl and decaprenyl diphosphate synthases in mice and humans We show that these enzymes are heterotetramers, like the decaprenyl diphosphate synthase ... synthases in mice and humans need two proteins (i.e both mSPS1 and mDLP1 or both hDPS1 and hDLP1, respectively) to be active The success of reconstitu...

Ngày tải lên: 16/03/2014, 23:20

... agglomerate and form fusiform nanocrystallines Finally, the ␣ -FeOOH Fig The EDS spectra of the as-prepared porous fusiform ␣-Fe2 O3 nanocrystallines 220 X Liu et al / Journal of Alloys and Compounds ... pattern of the rodlike ␣ -FeOOH nanocrystallines taken on a mass of ␣ -FeOOH nanorods reveals the satisfactory crystallinity of the sample, which can be indexed to the...

Ngày tải lên: 19/03/2014, 16:48

... 40 and 90 nm (see Fig 2b and c) SN, SN-650 and SN-850 powders showed speci®c surface areas of 31, 31 and 17 m2 gÀ1, respectively W 1-6 50 and W 5-6 50 pellets showed speci®c surface area of 25 and ... representing the line of absorbance zero in Fig 5a, a broad, weak, and negative 93 Fig Time resolved effects of mbar of NO2 at RT on FT-IR spectra of (a) SN-650, (b...

Ngày tải lên: 20/03/2014, 13:06

... for recombinant and native GAPDH with a multit using a common value of Km and different values of kcat The estimated parameters had a value of 25 lM for the Km, and the kcat for recombinant and ... those of native GAPDH The decrease of the catalytic constant is a fast process compared to the decrease of the K0.5 for BPGA These changes are...

Ngày tải lên: 23/03/2014, 20:22

... amphibians, and a third for invertebrates in which Artemia ferritin and artemin reside (Fig 6) Artemin and Artemia ferritin are most closely related to the Drosophila ferritins, with artemin and one of ... 5¢ end and DNA Strider 1.2 for analysis [38] Structural characterization of artemin and ferritin cDNAs and proteins Of the 672 analyzed clones in the...

Ngày tải lên: 23/03/2014, 20:22

... transhydrogenase assay described by Gan et al [25] Cloning of the two genes from A pernix K1 Thioredoxin reductase activity assays Assays for thioredoxin reductase activity were carried out by two ... 2002 Thioredoxin system of Aeropyrum pernix (Eur J Biochem 269) 5425 Thioredoxin activity assays RESULTS Thioredoxin activity was determined by the insulin precipitation assay...

Ngày tải lên: 23/03/2014, 21:20

... Lee, and Guodong Chen ț Characterization of Impurities and Degradants Using Mass Spectrometry Mike S Lee and Mingshe Zhu ț Mass Spectrometry in Drug Metabolism and Disposition: Basic Principles and ... ejection, and various forms of ion manipulation MASS SPECTROMETER TYPES 1.3.3 19 Time -of- Flight Mass Spectrometers The premise of separations by time -of...

Ngày tải lên: 28/03/2014, 10:20

... this minireview is to summarize what is currently known about the molecular mechanisms of osmotic stress resistance, osmosensing and signal transduction in C elegans Osmosensing and signaling in ... process of interest, allows genes to be ordered into pathways, and can provide important and novel mechanistic insights into the molecular structure and functio...

Ngày tải lên: 30/03/2014, 03:20