nmr characterization of cereal and cereal products
Báo cáo khoa học: Total chemical synthesis and NMR characterization of the glycopeptide tx5a, a heavily post-translationally modified conotoxin, reveals that the glycan structure is a-D-Gal-(1fi3)-a-D-GalNAc pot
Ngày tải lên :
23/03/2014, 13:20
... increments in t1 and spectral widths of 8000 Hz and 17 591 Hz in the 1H and 13C dimensions, respectively A total of 256 summed scans were collected with a relaxation delay of 1.3 s All 1H and 1H-13C ... between GNH3 of GalNAc and GH1 of Gal, which is labeled in bold The amino acids Gly6, Thr10 and Pro13 are represented by 6G, 10T and 13P Ó FEBS 2004 Glycopeptide tx5a synthesis and NMR analysis ... part of the INSIGHT suite of programs (Accelrys, San Diego, CA, USA) Ó FEBS 2004 Glycopeptide tx5a synthesis and NMR analysis (Eur J Biochem 271) 4943 Results After synthesis and deprotection of...
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Báo cáo y học: "Characterization of N200 and P300: Selected Studies of the Event-Related Potential Salil H. Patel 1 and Pierre N. Azzam 2"
Ngày tải lên :
02/11/2012, 11:08
... relationship of age, gender, handedness, and sidedness to the size of the corpus callosum Academic Radiology 1994; 1:243-248 69 Driesen NR, Raz N The influence of sex, age, and handedness on ... levels of active latency were associated with concentration ability, and passive latency with verbal proficiency and recall [72] The decreases in P3 and novelty P3 with increasing age, and indeed ... descriptive and limited in power and generalizability Still lacking are large-scale, blinded, randomized, prospective studies of ERP-guided therapy; however with decreasing costs of signal acquisition and...
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Báo cáo khoa học: Thermodynamic characterization of substrate and inhibitor binding to Trypanosoma brucei 6-phosphogluconate dehydrogenase pot
Ngày tải lên :
07/03/2014, 05:20
... injections of 10 lL of ligand, at 380 s intervals An initial preinjection of lL volume was made, and the result from this injection was not used for data analysis Heats of dilution and mixing, ... present In the case of 4PE, the Kd value of the inhibitor is close to the Kd value of the substrate, but it decreases by two orders of magnitude in the presence of both oxidized and reduced coenzyme ... concentrations of 5PR and 4PE were determined by measuring the concentration of organic phosphate [27], 6PG and NADP were determined enzymatically, and the concentrations of aPyADP and NADPH were determined...
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Báo cáo khoa học: 15 N-Labelled proteins by cell-free protein synthesis Strategies for high-throughput NMR studies of proteins and protein–ligand complexes doc
Ngày tải lên :
07/03/2014, 12:20
... Ala and Glu, Glu and Asp, and Glu and Gln is efficient in wheat germ extract but can effectively be suppressed by inhibitors of transaminases and glutamine synthase [20] Among the multitude of ... observation and dynamics of specific amide protons in T4 lysozyme Biochemistry 24, 817–822 McIntosh LP & Dahlquist FW (1990) Biosynthetic incorporation of 15N and 13C for assignment and interpretation of ... resonance assignments of the [15N]-HSQC spectrum, where it is often difficult to assess the magnitude of chemical shift changes upon ligand binding in [15N]-HSQC spectra of uniformly labelled...
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Báo cáo khoa học: Characterization of structural and catalytic differences in rat intestinal alkaline phosphatase isozymes pdf
Ngày tải lên :
07/03/2014, 17:20
... two AP monomers of AP together [32] In a variety of human cancer cell lines and sera, the Kasahara AP isoform has been found to consist of heterodimers of hIAP and hPLAP [33,34], and the human ... one rIAP band, with an apparent molecular mass of 70 kDa, was present, but two bands of 88 kDa and 75 kDa were detected as rIAP-II and rIAP-I in the duodenum The molecular mass values of the rIAPs ... heterodimers of hIAP and hPLAP [35] Ovarian cancer cells and cell lines derived from these cells express heterodimers of hPLAP and hGCAP [36–38] While no heterodimers of rIAP-I and rIAP-II have...
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Báo cáo khóa học: C-, 15N- and 31P-NMR studies of oxidized and reduced low molecular mass thioredoxin reductase and some mutant proteins docx
Ngày tải lên :
16/03/2014, 16:20
... 31P -NMR spectrum of wild-type TrxR in the oxidized (A) and the reduced state (B) Table 31P -NMR chemical shifts (in p.p.m.) of native and native reconstituted thioredoxin reductase (TrxR), and of ... TARFH2 and FMNH2, are C(5a), C(7), C(9a) and C(10a), and as already mentioned above, C(2) (Fig 7B) With respect to the 13C chemical shifts of FMNH2, C(2), C(5a), C(9a) and C(10a) of wild-type and ... P -NMR analyses The 31P -NMR spectra of the native wild-type TrxR from E coli in the oxidized and reduced state are shown in Fig The 31P -NMR spectrum of the pyrophosphate moiety of FAD bound to oxidized...
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Báo cáo khoa học: Characterization of solanesyl and decaprenyl diphosphate synthases in mice and humans docx
Ngày tải lên :
16/03/2014, 23:20
... hDPS1 were 1230 and 1245 bp, respectively, whereas those of mDLP1 and hDLP1 were 1206 and 1200 bp, respectively The mSPS1 and hDPS1 genes were 83.0% identical, and their translated products were ... humans and S pombe (A) Q10 standard, (B) wild-type (WT), (C) E coli harboring pBSDPS1 and pSTVhDLP1, (D) pBmSPS1 and pSTVhDLP1, (E) pBSDPS1 and pSTVmDLP1, (F) pUhDPS1 and pSTVmDLP1, (G) pBmSPS1 and ... with SalI and BamHI and cloned into the same sites of pREP2 and pBluescript KS+ to yield pRmDLP1 and pBmDLP1, respectively To make pET-mDLP1, pBmDLP1 was digested with SalI and NotI and then cloned...
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Báo cáo Y học: Characterization of the self-splicing products of two complex Naegleria LSU rDNA group I introns containing homing endonuclease genes pdf
Ngày tải lên :
18/03/2014, 01:20
... Rotaliella and Skeletonema [24,25] and only one nucleotide downstream of the well studied nuclear group I introns in Physarum and Tetrahymena [20,26] Four out of nine analyzed strains of this particular ... Primary sequences of I-NaeI and I-NmoI Putative DNA binding domain and zinc binding motifs (Zn-I and Zn-II) are underlined (B) Sequence comparison of I-NaeI, I-NmoI, I-PpoI [5] and I-NjaI [33] ... sites 3¢ of positions C549 and C550 of Nmo.L2563 (Fig 5B) These sites correspond very well to the reported internal processing sites of the Ppo.L1925 intron [11,20] and circularization sites of Tth.L1925...
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hydrothermal synthesis and characterization of - feooh and fe2o3 uniform nanocrystallines
Ngày tải lên :
19/03/2014, 16:48
... / Journal of Alloys and Compounds 433 (2007) 216–220 Fig (A) TEM image of the sample produced at 140 ◦ C for h (B) TEM image of the sample produced at 140 ◦ C for h The insets of (A and B) show ... The resulting products were filtered, washed with distilled water and absolute ethanol, and finally dried in vacuum at 50 ◦ C for h 2.2 Characterization The rodlike ␣-FeOOH (goethite) and porous fusiform ... phase of hematite ␣-Fe2 O3 and exhibits that each porous fusiform ␣-Fe2 O3 nanocrystalline was a single crystal Fig 5A and B shows scanning electron microscope images of a typical sample of fusiform...
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preperation and characterization of sno2 and wox - sno2
Ngày tải lên :
20/03/2014, 13:06
... 40 and 90 nm (see Fig 2b and c) SN, SN-650 and SN-850 powders showed speci®c surface areas of 31, 31 and 17 m2 gÀ1, respectively W1-650 and W5-650 pellets showed speci®c surface area of 25 and ... Effects of mbar of the NO2/O2 mixture at 1508C on FT-IR spectra of SN-650 and W5-650 pellets (curves and 2, respectively) The dotted line represents the zero of absorbance 3.3 Electrical characterization ... temperatures of 3008C for 100 ppm of CO (Fig 10) and at 2008C for 10 ppm of NO2 (Fig 11) in wet air (40% RH) are reported We have shown these tests (300 instead of 2508C for CO and 200 instead of 1508C...
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Báo cáo khoa học: NMR study of cellulose and wheat straw degradation by Ruminococcus albus 20 pdf
Ngày tải lên :
23/03/2014, 07:20
... quantification of fermentation products performed by both 1H NMR (Figs and 2) and enzymatic methods Figure shows an example of the spectra obtained before and after days of culture of R albus 20 ... degradation and metabolism of cellulose and wheat straw by R albus 20 cells growing on these substrates were studied using NMR A kinetic analysis of the polysaccharides degraded and of the sugars ... centrifugation, was freeze-dried and analysed further by 13C-CP MAS NMR The quantification of the 13C signals of the crystalline and amorphous zones obtained on pellets of both cellulose and 13C-labelled wheat...
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Báo cáo khoa học: Characterization of native and recombinant A4 glyceraldehyde 3-phosphate dehydrogenase Kinetic evidence for conformation changes upon association with the small protein CP12 pptx
Ngày tải lên :
23/03/2014, 20:22
... recombinant and native GAPDH with a multit using a common value of Km and different values of kcat The estimated parameters had a value of 25 lM for the Km, and the kcat for recombinant and native ... at 42.5 2.8 kDa A 1-L culture of E coli yielded mg of pure GAPDH with a specic activity of 146 11 Uặmg)1 when NADPH was used as cofactor and a specic activity of 35 Uặmg)1 when NADH-dependent ... phosphoglycerate kinase and 33 mM ATP in a nal volume of 1.5 mL at 30 C for 20 The concentration of BPGA in the presence of 0.25 mM NADH was determined using excess rabbit muscle GAPDH and 10 lL of the previous...
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Báo cáo khoa học: Molecular characterization of artemin and ferritin from Artemia franciscana pot
Ngày tải lên :
23/03/2014, 20:22
... [24–27] Helices A and B are antiparallel, as are C and D, and they are connected by small loops A large loop, designated L, connects A and B helices with C and D helices, and L loops of neighboring ... heat and chemicals such as urea and guanidinium chloride [24], and the degradation of ferritin in vivo is inhibited by excess iron [32] To address questions of structure and function, artemin and ... 5¢ end and DNA Strider 1.2 for analysis [38] Structural characterization of artemin and ferritin cDNAs and proteins Of the 672 analyzed clones in the Artemia EST library, one artemin and two...
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Báo cáo Y học: Identification and characterization of thioredoxin and thioredoxin reductase fromAeropyrum pernixK1 pdf
Ngày tải lên :
23/03/2014, 21:20
... dithiothreitol in a total volume of 50 lL Then, 40 lL of a reaction mixture composed of 200 lL of Hepes (1 M), pH 7.6, 40 lL of EDTA (0.2 M), 40 lL of NADPH (40 mgÆmL)1), and 500 lL of insulin (10 mgÆmL)1) ... with the addition of 10 lL of thioredoxin reductase, and incubation was continued for 20 at 37 °C The reaction was stopped by the addition of 0.5 mL of M guanidine-HCl and mM NBS2, and the absorbance ... ApTR (B) Molecular mass determination of ApTrx and ApTR Molecular masses of recombinant ApTrx and ApTR were determined by analysis of the elution files of standard proteins from a Sephacryl S-200...
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Base Working Paper on Strategy and Action Plan for Ensuring Safety of Milk and Milk Products ppt
Ngày tải lên :
24/03/2014, 04:20
... Exports and imports of milk and milk products Problems in export and import of milk and milk products Objective 3: Current enforcement procedures and Prevention of Food Adulteration (PFA) Act and ... mandates application of the standards of the Bureau of Indian Standards (BIS) for milk powders, evaporated and condensed milks The Directorate General of Health Services, Ministry of Health and ... for Analysis and Learning in Livestock and Food whose mandate is to offer services on analysis of milk products and other food products for various safety and quality parameters, and carrying...
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CHARACTERIZATION OF IMPURITIES AND DEGRADANTS USING MASS SPECTROMETRY pot
Ngày tải lên :
28/03/2014, 10:20
... ejection, and various forms of ion manipulation MASS SPECTROMETER TYPES 1.3.3 19 Time -of- Flight Mass Spectrometers The premise of separations by time -of- flight MS (TOF-MS) is that a mixture of ions of ... Structural Identification of Extractable 11.4 New Degradation Products and Pathways of Vitamin D and Its Analogs 11.4.1 Thermal Isomerization of Vitamin D3 in DMSO 11.4.2 Autoxidation of Isotachysterol ... hallmark of impurity and degradant analysis HPLC is often used to profile and classify molecules and work in concert with mass spectrometry to assist with the elucidation of structure Identification of...
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Báo cáo khoa học: Quenched hydrogen ⁄deuterium exchange NMR characterization of amyloid-b peptide aggregates formed in the presence of Cu2+ or Zn2+ ppt
Ngày tải lên :
30/03/2014, 01:20
... between 200 and 250 nm, and averaged over 10 scans with a bandwidth of nm, a response time of s, a pitch of 0.5 nm, and a scan rate of 20 nmÆs)1, in a mm quartz cuvette AFM A portion of each Ab ... Zn2+ and Fe2+ are found within deposited plaques in vivo [20], and both Cu2+ and Zn2+ affect the toxicity of Ab [21–25] The in vivo effect of metals can be complex [26,27], and the presence of ... aggregation of Ab in vitro [19,28,29] The binding of Cu2+ and Zn2+ to monomeric Ab has been extensively studied and occurs via three histidines (His6, His13, and His14) and a fourth ligand suggested...
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Báo cáo khoa học: Molecular and genetic characterization of osmosensing and signal transduction in the nematode Caenorhabditis elegans docx
Ngày tải lên :
30/03/2014, 03:20
... genes also causes accumulation of damaged proteins and activation of gpdh-1 expression Green arrows and red lines indicate activation and inhibition, respectively Role of cuticle collagens in regulating ... proteins [11] Mutant alleles of three of these genes, osr-1, osm-7 and osm-11, have been characterized and shown to cause constitutive accumulation of glycerol [25,29] osr-1 and osm-7 are expressed ... analysis of the WNK-1 ⁄ GCK-3 pathway that regulates hypertonic stress responses Conclusions and future perspectives Fig Model of GCK-3 and WNK-1 function in the hypodermis and intestine of C elegans...
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Báo cáo khoa học: Expression and functional characterization of P2Y1 and P2Y12 nucleotide receptors in long-term serum-deprived glioma C6 cells ppt
Ngày tải lên :
30/03/2014, 08:20
... proliferative and differentiation state, basal level of ERK1 ⁄ and Akt kinase phosphorylation, and P2Y1 and P2Y12 receptor protein expression and functional activity Examination of the effects of specific ... ⁄ G1, 25% in G2 ⁄ M, and 15% in S phase After 24 and 48 h of serum starvation, 80% and 90% of cells were growth arrested, respectively However, there were still a number of unsynchronized cells, ... [phosphatase and tensin homolog (PTEN)] [3] PTEN dephosphorylates the 3¢ position of phosphoinositides and acts as an antagonist of PI3-K, resulting in a reduction of the concentration of this kinase...
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Báo cáo khoa học: Stoichiometry of LHCI antenna polypeptides and characterization of gap and linker pigments in higher plants Photosystem I doc
Ngày tải lên :
30/03/2014, 15:20
... significant loss of pigments, suggesting that ÔgapÕ chlorophylls are a unique characteristic of PSI Materials and methods Purification of the native and recombinant complexes PSI–LHCI complex and its PSI ... using a acrylamide/bis-acrylamide ratio of 75 : and a total concentration of acrylamide + bis-acrylamide of 4.5% and 15.5%, respectively, for the stacking and running gel Urea (6 M) was also incorporated ... (0.35 lg of Chls loaded in lanes and 7, 0.47 lg in lanes and 9, 0.6 lg in lanes and 11) are shown On the right, the mobility of Lhca1 band in recombinant sample and PSI–LHCI complex is reported,...
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