Paired end transcriptome assembly and genomic variants management for next generation sequencing data

... PETA and UASIS, to interpret and analyze large scale of Next Generation Sequencing data They serve as fundamental components to provide accurate transcriptomes and better data management for related ... PETA (Paired End Transcriptome Assembler) We claim that the full utilization of raw reads and paired- end information is able to construct a cleaner splicing grap...

Ngày tải lên: 01/10/2015, 17:28

... frequent in reads that align to the SNP site only at later sequencing cycles, indicating a technical bias in base calls at this position, while the plot in (b) shows a strong reduction in this bias In ... Durbin R: Mapping short DNA sequencing reads and calling variants using mapping quality scores Genome Res 2008, 18:1851-1858 doi:10.1186/gm208 Cite this article as: Taub...

Ngày tải lên: 11/08/2014, 12:21

... due to sequencing errors For NGS sequencers like Illumina and SOLiD, the majority of sequencing errors are of this type The first contribution of this thesis is the introduction of a fast and memory-efficient ... overview of the importance and applications of genomic sequencing We will now present a review of the technologies behind genome sequencing 2.4.1 Sanger S...

Ngày tải lên: 10/09/2015, 09:11

... caacctctgggttcagcttttgccaagcttcagGTGTTTGCACACCGTTAGAAATTACCACAAATGGTTGAAAAATC T S G F S F C Q A S G V C T P L E I T T N G STOP NUP214 (exon 29) XKR3 (exon 4) caacctctgggttcagcttttgccaagcttcagCATTGCTGATGACATTTTCCCTGTTATCAGTTACTTATGGGGC ... cDNA libraries PR and AM performed the hybrid selection MFB and JZL analyzed the sequence data XA and AG confirmed fusion transcripts and SNPs...

Ngày tải lên: 09/08/2014, 20:20

... (http://www.amkor.com/products/notes_papers/IMAPS_tsCSP_paper_Solectron.pdf) 10 Assembly and PCB Layout Guidelines for QFN Packages Appendix I: Detailed PCB Layout Figure 12 shows the PCB top layer of a suggested board layout of soldered pads for QFN packages This ... Soldered Pads for QFN Packages, Reduced Thermal Pad (PCB top layer) 12 Assembly and P...

Ngày tải lên: 08/08/2014, 12:22

... 67 Reference Material 66 67 Ethernet Access for Next Generation Metro and Wide Area Networks OL-14760-01 iii Ethernet Access for Next Generation Metro and Wide Area Networks Introduction Scope ... tabular format in Table Ethernet Access for Next Generation Metro and Wide Area Networks 40 OL-14760-01 Scalability and Performance Resu...

Ngày tải lên: 21/12/2013, 06:15

Ngày tải lên: 22/12/2013, 10:16

... Detection System; shALIGN: shRNA Alignment; shRNA: short hairpin RNA; shRNAmir: shRNA encoded within a miRNA; shRNAseq: shRNA Sequencing; sinLTR: self-inactivating LTR; TAMRA: tetramethylrhodamine; ... massively parallel sequencing - 31 - shRNA library glycerol stock shRNA library plasmid shRNA library virus Image analysis Target cell infection Base calling e ca Puromycin select...

Ngày tải lên: 09/08/2014, 23:20

... Investigating lipid and secondary metabolisms in plants by next- generation sequencing JIN JINGJING (B.COMP., SCU) (B.ECOM., SCU) A THESIS SUBMITTED ... ligation and singlemolecule sequencing Sequencing by synthesis involves taking a single strand of the DNA to be sequenced and then synthesizing its complementary strand enzymatically The pyrosequencing ... and p...

Ngày tải lên: 09/09/2015, 11:16

... binding domain, and the ligand binding domain (LBD) The “hinge domain” links the LBD to the DBD (42) (Figure 6) Figure 6: Schematic representation of AR on Chromosome X (43) Upon the binding of ... CTD-2310E8 RP11-188K5 Invitrogen Invitrogen Invitrogen Invitrogen Invitrogen Invitrogen Invitrogen Invitrogen Invitrogen   33     Methods 6.1 Next generation sequencing (SOLiD4) DNA Extra...

Ngày tải lên: 19/11/2015, 14:04

... in MIPv6: Binding cache: Binding cache is maintained by HAs and CNs A binding cache is used to hold the binding for MNs If a node receives a BU destined for it, it will add the binding

Ngày tải lên: 26/11/2015, 12:30

... Identification Signature with Paired- End Tags DNA-PET Genomic DNA analysis with Paired- End Tags GSC-PET Gene Scanning CAGE with Paired- End Tags GST Genomic Signature Tags iPET Inter-ligation PET ... Polyadenylation Site PCR Polymerase Chain Reaction PE-GST Paired End Genomic Signature Tags PEM Paired End Mapping PES Paired End Sequencing PET Paired- E...

Ngày tải lên: 14/09/2015, 14:13

... eukaryotic genomes 18 2.5 EULER-USR EULER-USR [5] is in many ways similar to Velvet It is a set of tools designed to carry out de novo assembly of paired and non -paired short reads using the De Bruijn ... this is not attainable using reads as short as 25bp Finally, they were not explicitly designed to take advantage of paired reads Therefore new approaches were nee...

Ngày tải lên: 04/10/2015, 10:25

Ngày tải lên: 21/09/2012, 11:02

... SELF-ASSEMBLY AND NANOTECHNOLOGY SELF-ASSEMBLY AND NANOTECHNOLOGY A Force Balance Approach Yoon S Lee Scientific Information Analyst Chemical Abstracts Service A Division of the American Chemical ... Range and magnitude are relative scales der Waals and electric double-layer forces are exerted at long and wide ranges, the hydration, solvation, and depletion forces hav...

Ngày tải lên: 15/11/2012, 10:12