Survey of cellular factors modulating the HIV 1 integration complex activity using a unique protein screening system in vitro

... ubiquitin ligases in the degradation of HIV- 1 IN 14 1. 6 HIV- 1 PIC as a better target of study than recombinant IN 17 1. 6 .1 Cellular components and modulators of the pre -integration nucleoprotein complex ... Structural domain of HIV- 1 IN IN contains 288 amino acid residues and has three protein domains The NTD facilitates protein dimerizati...

Ngày tải lên: 01/10/2015, 17:27

... reports of siRNA/ shRNA screens for HIV-1 replication cofactors are therefore not the beginning of the end, but the end of the beginning of a new era in the search for host factors required for HIV-1 ... exhaustive The goal of siRNA and shRNA screens is to identify all possible HIV-1 cofactors For productive replication to occur, HIV-1 has to switc...

Ngày tải lên: 12/08/2014, 23:22

... Since apoptosis mediated by Tat partly relies on the interaction of Tat with tubulin [14-17], we hypothesized that zinc binding might play a role in the modulation by Tat of the microtubule dynamics ... 5:62 Consequently, holo -Tat likely constitutes the active form of Tat in the cell cytoplasm By altering the microtubule dynamics, holo -Tat may t...

Ngày tải lên: 13/08/2014, 05:21

... FACS analysis of the internalization of the full-length Tat protein construct and the Tat CPP Differences in the mechanisms of internalization between the Tat peptide and the Tat fused protein ... at the surface of the Tat- containing recombinant proteins and call other reasons to explain the differences in the mechanism of interna...

Ngày tải lên: 21/02/2014, 03:20

... our mapping of the MA binding site on the EED linear sequence, since β-strands d were the most exposed β-strands at the periphery of the β-propeller (Fig 2D) Of note, the upper face of the β-propeller ... β-propeller domain of EED and protein-interacting regions Figure Surface representation of the β-propeller domain of EED and protein-...

Ngày tải lên: 20/06/2014, 01:20

... added a biotinylable tag at the C-terminus of IN (IN- BAD) and showed that the protein remains fully active in the context of a lentiviral vector The kinetics of viral DNA synthesis and integration ... shown) The presence of LEDGF/p75 in infected cells prevents access to the IN C-terminus We next asked whether the presence of LEDGF/p75 in cells lysate...

Ngày tải lên: 12/08/2014, 23:23

... necessary and sufficient for Rev nuclear import inhibition by HIC Nuclear imports of GST-YFP -Rev, GST-YFP -Rev N1, GST-YFP -Rev N2 and GST-YFP-RevNLS were examined using in vitro nuclear import assays ... pathways or the physical obstruction of the NPC since M9 or Rev nuclear import mediated by transportin remained unaffected by HIC The molecular recognition of...

Ngày tải lên: 13/08/2014, 01:20

... [33] The surface of interaction of the IBD with a subset of these proteins partially overlaps with that of integrase (reviewed in [19]) The affinity of IBD for integrase is higher than for some of ... integrase The HIV-1 co-factor activity of LEDGF/p75 involves its simultaneous interaction with the host chromatin and with HIV-1 integrase [1,2]...

Ngày tải lên: 13/08/2014, 01:20

... manuscript YW and XQ constructed and characterized the HIV-1 viruses and lentiviral vectors used in the study CS performed the analysis of DNA microarray data and performed the statistical analysis AR ... protocol (Promega), and the products were measured by a luminometer (Turner) Microarray analysis Microarray analysis was performed by the Baylor Microarray Core Facility (B...

Ngày tải lên: 13/08/2014, 09:20

... Ser146 in the numbering of the intact CA) The CACW40A protein is monomeric, and its structure is similar to that of the subunits in the dimeric, non-mutated CAC, but, in the monomeric form, the segment ... responsible, from a thermodynamic point of view, for the binding of the monomeric species of CAC? We have previously discussed the variation in the fr...

Ngày tải lên: 07/03/2014, 06:20

... to the processed DNA product The stability of IN during the time-course of the reaction and the inuence of protein aggregation are discussed Results General features of the 3Â-end processing kinetics ... clear In the case of IN, tight binding of the protein to recessed viral DNA ends is obligatory in the context of the HIV replication cycle: the 3Â -p...

Ngày tải lên: 16/03/2014, 13:20

... with the symmetry of space group C2221 Overall hydrophobic NNRTI interactions to wild-type and K103N mutant RT The interactions of Efavirenz, MSC194 and PNU142721 to wild-type RT and the K103N mutant ... study we present the structural indications for the role of K103 and N103 in drug binding and the structural implications for the inhib...

Ngày tải lên: 18/03/2014, 01:20

... which was slightly active on the wild-type RT RNase H function, showed a sixfold increase in the inhibition potency of the RNase H function, although it retained its inhibition potency on the ... AQ derivatives and other RT inhibitors (A) YonetaniTheorell plot of the combination of K-49 and RDS 1643 on the HIV-1 RT polymeraseindependent RNase H a...

Ngày tải lên: 22/03/2014, 16:20

... on the 3Â-end processing step (not shown) The effect of peptides on the strand-transfer reaction When the effects of the various peptides on the strandtransfer step of the IN enzymatic reaction ... group of peptides is attributable to their effect on the turnover number of IN, and suggest a different mode of action from that of the rst group Th...

Ngày tải lên: 22/03/2014, 16:21

... binding of NCp7 to the C-terminal domain (80–96 )Vpr [8] may expose the N-terminal (1–39) portion of the protein and allow its tight binding to Lys-tRNA synthetase [15] The C-terminal domain (52–96 )Vpr ... when comparing the isolated domains of (1–51 )Vpr [25] and (52–96 )Vpr [26] with the intact Vpr protein The differences in structure are loc...

Ngày tải lên: 31/03/2014, 23:20