The ph dependent stability of DNA i motif

... distributions strongly depend on pH conditions In certain pH and loading rate conditions, the unfolding forces distribution of i- motif show two peaks, indicating that the unfolding of i- motif ... took the gradient direction of magnetic field to be z direction, which is also the direction of force Since we have had x and z 25 direction, we just chose y direction in...

Ngày tải lên: 30/09/2015, 10:13

... 2002 Aromatic stacking in API (Eur J Biochem 269) 4153 Fig Stick models of the reactive site in bovine trypsin and API The catalytic triad residues of trypsin and API are Ser195–His57– Asp102 and ... because several serine proteases not have an aspartate as the catalytic apparatus M NaCl However, for chymotrypsin-type serine proteases, the replacement of this aspar...

Ngày tải lên: 21/02/2014, 03:20

... stoichiometries of participation of water, cations and anions in specific and non-specific binding of lac repressor to DNA Possible thermodynamic origins of the ‘‘glutamate effect’’ on protein–DNA ... function optimally Effects of mutations The point mutations are not in the immediate vicinity of the active site situated at the bottom of the positively charge...

Ngày tải lên: 16/03/2014, 06:20

... studied the thermal unfolding of both the wild-type HUTmar protein and its E34D mutant in acidic solutions (pH 4.00, 3.75 and 3.50) to improve the reversibility of unfolding and avoid postunfolding ... We report here the results of an extensive thermalstability study of a hyperthermophilic protein, the histonelike protein from T maritima (HUTmar-wt...

Ngày tải lên: 23/03/2014, 12:20

... nuclei were obtained by treatment of solution A (pH 7.6) containing 1,000 ml of the stock solution and 30 mg of trypsin The trypsinization by solution A increased the fluorescence of nuclei with ... population consisting of round/elongating spermatids and elongated spermatids In rats older than weeks, the proportion of elongated spermatids increased steadily The ratios...

Ngày tải lên: 07/08/2014, 14:23

... resulting in the accumulation of both unmodified cholesteryl esters and glycated apoB in the cells The rate of removal of the latter species is slow, with only 20–30% of the glycated protein being ... although the concentrations of these materials present in the artery wall, and in atherosclerotic lesions, are unknown The role of glycation and...

Ngày tải lên: 19/02/2014, 02:20

... activity in crude yeast extract as the untagged protein, indicating that the tag was not interfering with the protein activity The tagged protein was purified and then used for further analysis In the ... nkatÆmg)1 The GLD1 protein was tagged with a histidine tag at the N-terminal end by adding the coding sequence for six histidines to the end of the open readin...

Ngày tải lên: 19/02/2014, 06:20

... Structure of human wild-type lysozyme and location of the mutations discussed in this study The locations of the single-point mutations are shown on the structure of human wild-type lysozyme, defined by ... demonstrate a clear relationship between the levels of protein secreted from P pastoris and the native-state thermostability of the lysozyme va...

Ngày tải lên: 19/02/2014, 08:20

... hydrophobic core (M88V) and the other in the protein–RNA interaction (T45S), decrease the stability of the capsid Our findings illuminate the role of packing in the icosahedral lattice of the virus ... middle of the central b-strand of the coat protein dimer and its side-chain projects into the protein’s interior (Figs 1B and 8) The two Met...

Ngày tải lên: 19/02/2014, 12:20

... helix of the third cluster of CHCs is part of the inter-domain segment and the other helix lines the boundary between the major domain and the N-terminus (Fig 1) Therefore, the formation of the ... rather than functional role Role of hydrophobic contacts in serine hydroxymethyltransferase In the present study, the importance of the third hydrop...

Ngày tải lên: 07/03/2014, 03:20

... detailed knowledge on how Zn2+ ions modulate the conformation and stability of S100A2 will contribute to a better understanding of the regulation of protein function by metal ions, in particular as a ... be suggestive of partial tetramerization (Fig S1) Chemical stability of holo and apo S100A2 proteins The conformational stability of S100A2- wt and...

Ngày tải lên: 30/03/2014, 02:20

... blotting with anti -Cdc37 Samples were analyzed after dephosphorylation for (lanes and 7), (lanes and 8), 10 (lanes and 9), and 20 (lanes and 10) antibody against Cdc37 and the only band that became ... activate a kinase By contrast, binding an activating regulatory coprotein to an inactive catalytic subunit can activate a kinase In many signal-transducing protein kin...

Ngày tải lên: 30/03/2014, 03:20

... analysis of the processivity and the fidelity of DNA synthesis (namely, the ability of BLV RT to misincorporate nucleotides at the 3¢ end of the growing DNA strand and the further extending of the ... multiple-round synthesis (Fig 1) The analysis of the processivity of DNA synthesis in the presence of a DNA trap suggests that BLV RT...

Ngày tải lên: 31/03/2014, 21:21

... designs, the resistance against axial rotation was assessed using FEM on the one hand and the analytical model on the other With respect to the FE models, the analytical model predicts a very stiff stability ... loads The run time of an FE analysis ranged from 45 minutes up to 60 minutes The run times of the MATLAB implementation of the analytical model...

Ngày tải lên: 20/06/2014, 01:20

... using the fixed point alternative approach, we prove the Hyers-Ulam stability of the functional equation (1) in fuzzy normed spaces In the rest of the article, assume that X is a vector space and ... Non-Archimedean stability of the functional equation (1) In this section, using the fixed point alternative approach, we prove the Hyers-U...

Ngày tải lên: 20/06/2014, 22:20