Accurate gene and miRNA quantification in neuronal differentiation

... signaling Figure 1.2 Outline of miRNA biogenesis and action Figure 1.3 Involvement of miRNAs in control of neuronal differentiation Figure 2.1 Overview of gene expression during neuronal ... dendritic spines [71] and synapse activity [72] Figure 1.3 Involvement of miRNAs in control of neuronal differentiation miR-9 and miR-124 promote neuronal differentiation by re...

Ngày tải lên: 26/09/2015, 10:51

... pathways involved in the activation of specific proteins, mRNAs and miRNAs through combinatorial interactions of GFLs, GFRα and RET receptor isoforms and provides novel insights into the diverse functions ... ligand receptor systems and microRNAs during neuronal differentiation of NTera2 neuroprogenitor cells (Chapter 8) The findings in this thesis further...

Ngày tải lên: 09/09/2015, 10:13

... 1.3 GDNF receptor complex in neuronal biology Since the discovery of the roles of GDNF in promoting survival and differentiation of midbrain dopaminergic (DA) neurons and increasing the affinity ... NT2 into specific neuronal lineages and study the roles of GDNF receptor system in neuronal differentiation and neuronal lineage specification 38 CHAPTER GDN...

Ngày tải lên: 30/09/2015, 10:11

... Gene expressions of mature miR9 and miR9* during neuronal differentiation (A) Using real-time PCR The gene expressions of endogenous mature miR9 and miR9* during the neuronal differentiation of P19 ... for expression of pri-miR9-1 expression of pri-miR9-1 expression of pri-miR9-2 expression of pri-miR9-2 expression of pri-miR9-3 expression of...

Ngày tải lên: 18/02/2014, 17:20

... residing in both the inner and outer mitochondrial membrane, that is activated by mitochondrial Ca2+ overloading and other factors including oxidative stress [88,89] In neurons the presence of PTP in ... buffer the incoming calcium resulting in an abrupt, sustained and irreversible increase in [Ca2+]c, probably coincides with the cleavage of the exchanger (but see [1...

Ngày tải lên: 07/03/2014, 12:20

... gene signaling pathway analysis has identified gene signals that are remarkably activated in AT-MSC-Hepa, and these signals are also up-regulated in whole liver Therefore, the microarray analysis ... protocol, and then treated with deoxyribonuclease (DNase I, amplification grade; TaKaRa, Kyoto, Japan) Microarray analysis and data mining (Aligent array) A one-color microarray...

Ngày tải lên: 30/03/2014, 04:20

... clarify the role of BCL11B in T-cell malignancies, we further analyzed the expression levels of TNFSF10, BCL2L1, SPP1, and CREBBP genes and their correlations with BCL11B in patients with T-ALL and ... of the SPP1 gene in T-cell malignancies is unclear, because low expression of SPP1 was detected in T-ALL Conclusions The expression pattern...

Ngày tải lên: 10/08/2014, 22:21

... MPO polymorphism in the development of cognitive decline in MS This study was mainly designed to investigate whether some clinical and individual variables and the occurrence of the -463 G/ A promoter ... Ratios and CI were estimated using logistic regression adjusted for age and sex gene being involved in AD pathology, possibly through oxidation of Aβ or ApoE, promoti...

Ngày tải lên: 11/08/2014, 08:20

... by binning the data (in 100 bins) in both directions The intensity of blue represents the amount of points in the bin, as depicted in the legend refereed research Relative number of tissues, gene ... corresponding expression values of both duplicated genes and N is the number of samples In evaluating and comparing the distributions of the correlati...

Ngày tải lên: 14/08/2014, 16:21

... are involved in developmental regulation, including the timing and neuronal asymmetry in worm; growth control and apoptosis in fly; brain morphogenesis in zebrafish; and hematopoetic and adipocyte ... analysis points to an intricate network of transcription activators and repressors acting together with miRNAs in coordinating neuronal gene expression and pr...

Ngày tải lên: 14/08/2014, 17:22

... family, the miR-200 family have been reported in human [16,17] and mouse embryonic stem cells [18-20] The unique patterns of miRNA expression in embryonic stem cells suggest they are involved in maintaining ... instance, miR-373 induces the expression of E-cadherin and CSDC2 by targeting their promoter region and initiate their expression[ 73] Another me...

Ngày tải lên: 18/06/2014, 15:20

... of c-Jun, c-Fos and ATF2 29 1. 3 .1. 3 Regulation of AP- 1 activity by its interacting proteins 30 1. 3.2 Role of AP- 1 in cell proliferation and differentiation 31 1.3.2 .1 Role of AP- 1 in ... AP- 1 in cell proliferation 31 1.3.2 .1. 1 AP- 1 is an important regulator in cell proliferation 31 1.3.2 .1. 2 Mechanisms of AP- 1 modulation of...

Ngày tải lên: 16/09/2015, 15:54

... model 85 4.2 Temporal and spatial expression of nm23- M2 gene 85 4.3 The role of nm23- M2 in dopaminergic MN9D differentiation 87 4.4 The role of nm23- M2 in inducing cell cycle arrest 89 4.5 Further ... MN9D 64 cell differentiation 3.2 3.1.4 Spatial expression of nm23- M2 in MN9D and SH-SY5Y cells 66 3.1.5 Subcellular localization of nm23- M2...

Ngày tải lên: 08/11/2015, 17:09

... Mutations of the epidermal growth factor receptor gene and related genes as determinants of epidermal growth factor receptor tyrosine kinase inhibitors sensitivity in lung cancer Cancer Sci 98, ... Kinetic analysis of epidermal growth factor receptor somatic mutant proteins shows increased sensitivity to the epidermal growth factor receptor tyrosine k...

Ngày tải lên: 16/02/2014, 09:20

... Materials and reagents Fig Sensitivity to a-amanitin of the transcription of arginine decarboxylase (ADC) and cruzipain genes in transformed Trypanosoma cruzi Permeable parasites were preincubated ... Discussion Expression of the oat ADC gene in T cruzi In order to study the expression of the foreign ADC gene in T cruzi epimastigotes, we transformed...

Ngày tải lên: 19/02/2014, 07:20