Development and application of liquid phase microextraction techniques in the analysis of environmental pollutants

... drop -in- drop system The aqueous phase of the outer drop contained the analyte of interest and was continuously delivered and aspirated away throughout the sampling The analytical response of the instrument ... involves the use of hollow fiber combination with liquid- phase microextraction It can be categorized into two- ii phase microextraction, and three -phase microextraction or liquid- liquid -liquid microextraction ... aspiration of the ASP, following the first sampling cycle, ensures that both the OF and the ASP are periodically renewed, and thus the OF would be in contact with fresh aqueous sample having the initial...

Development and application of hollow fiber protected liquid phase microextraction for trace organic analysis

... Three -phase hollow fiber- protected liquid- phase microextraction techniques combined with HPLC analysis 42 2.1 Orthogonal array designs for the optimization of static three -phase hollow fiber- protected ... the hollow fiber This thesis reports on the development and application of hollow fiber- protected LPME techniques to trace organic analysis Chapter provides an introduction to extraction, and ... pores of hollow fiber, and the aqueous acceptor solution in the lumen of the hollow fiber was repeatedly moved in and out of the hollow fiber and the syringe Compared to the dynamic three-phase...

Development of liquid phase microextraction techniques combined with chromatography and electrophoresis for applications in environmental analysis

... DEVELOPMENT OF LIQUID- PHASE MICROEXTRACTION TECHNIQUES COMBINED WITH CHROMATOGRAPHY AND ELECTROPHORESIS FOR APPLICATIONS IN ENVIRONMENTAL ANALYSIS by ZHANG JIE (M.Sc.) A THESIS SUBMITTED FOR ... chromatography LC liquid chromatography LGLME liquid- gas -liquid microextraction LLE liquid liquid extraction LLLE liquid- liquid -liquid microextraction LODs limits of detection LPME liquid- phase microextraction ... microextraction techniques and solvent-based microextraction techniques A comparative analysis of several main developments of microextraction techniques is list in Table 1-2 1.3.1 Sorbent -phase microextraction...

Development and applications of novel liquid phase microextraction techniques

... fiber-protected liquid- phase microextraction Headspace -liquid- phase microextraction Local anaesthetics Liquid chromatography Liquid- liquid extraction Liquid- liquid -liquid microextraction Limits of Detection ... 1.1 Historical Development of Microextraction Techniques 1.2 Principles and Applications of SME and Membrane-Based LPME Techniques 1.3 Hyphenation and Automation 15 1.4 Comparison of SME, Membrane-Based ... Chapter Solvent Microextraction Techniques: Headspace Liquid- Phase Microextraction and Solvent-Drop Liquid- Phase Microextraction 2.1 Introduction Methods for the collection and analysis of soil sample...

Development and application of novel capillary electrophoresis techniques for analysis of DNA fragments and organic pollutants

... focused on the analysis of DNA fragments; and the second part (chapter and chapter 5) focused on the analysis of organic pollutants Several novel capillary electrophoresis (CE) techniques had ... various applications, the CE applications in DNA analysis and monitoring of pollutants are of significant importance 1.3.1 CE Application in DNA Analysis DNA separation by CE has been the subject of ... for on-site analysis of various pollutants at trace level CE is found to be a versatile analytical tool for the analysis of DNA as well as pollutants Combination of DNA analysis and environmental...

Development and application of advanced proteomic techniques for high throughput identification of proteins

... DEVELOPMENT AND APPLICATION OF ADVANCED PROTEOMIC TECHNIQUES FOR HIGHTHROUGHPUT IDENTIFICATION OF PROTEINS HU YI (B.Sc.) A THESIS SUBMITTED FOR THE DEGREE OF DOCTOR OF PHILOSOPHY DEPARTMENT OF ... analysis of proteins, i.e proteomics (Pandey and Mann, 2000; Tyers and Mann, 2003) Therefore, the advancement of proteomics relies largely on the development of state -of- the-art proteomics techniques ... study, we sought to develop and apply advanced proteomic techniques from three different aspects for high- throughput identification of enzymes and their associated proteins in yeast proteome (catalomics)...

the development and application of an antibody based biosensor

... spectrum of BT5AA 56 Synthesis and mass spectrum of 2TAA 58 Synthesis and mass spectrum of 3TAA 59 Synthesis and mass spectrum of 5M2TAA 60 Synthesis and mass spectrum of ... from the ancient remains of marine plant and animal life under extreme heat and pressure in an anaerobic environment Depending on the composition of the organic material, varying proportions of ... Schematic of a generalized biosensor The bioreceptor and the transducer are linked such that the transducer quantifies the biorecognition event occurring between the sample and the bioreceptor The...

Báo cáo hóa học: " Development and application of a biomarker assay for determining the pharmacodynamic activity of an antagonist candidate biotherapeutic antibody to IL21R in whole blood" pptx

... biomarkers, and they participated in the data analyses AAH developed the customized Spotfire tool used for data analyses and reviewed statistical analyses YG participated in the assessment and ... assay for determining the pharmacodynamic activity of an antagonist candidate biotherapeutic antibody to IL21R in whole blood Journal of Translational Medicine 2010, 8:51 Page 13 of 13 ... this basic approach (ex vivo stimulation of whole blood) to develop pharmacodynamic biomarker assays for a candidate therapeutic antibody, Ab-01 Ab-01, a human antibody generated by phage display,...

Báo cáo y học: "Development and application of versatile high density microarrays for genome-wide analysis of Streptomyces coelicolor: characterization of the HspR regulon" ppsx

... (and Lys) tend to be over- High density IJISS DNA arrays have been developed for global analysis of Streptomyces gene expression and transcription factor binding The HspR regulatory system of S ... against the test arrays and the probe performance was scored using the following equations Firstly the Cy3 and Cy5 background-subtracted signals, designated 'g' and 'r', respectively, obtained by feature ... chaperone and protease production It is envisaged that these arrays will find widespread use in systems level analysis of Streptomyces coelicolor biology Materials and methods Streptomyces strains and...

development and application of mixed beam-solid models for analysis of soil-pile interaction problems

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Combining DEMO models with RAD's techniques in the analysis phase of software development process

... difficulties in defining software requirements and business process modeling DEMO can help capture the business processes of the organization while RAD technique links these business processes to the software ... steps of the related transactions Capturing the business processes of the organization, it can be used as the starting point to design the supporting system for the organization Combining with ... improved Therefore, we have developed a new framework for the analysis phase of the software development process where we combined the IAM and the PM with the traditional RAD technique In every...

DEVELOPMENT AND APPLICATION OF NEW APPROACHES FOR STUDIES OF INFLUENZA INDUCED INFLAMMATION AND DNA DAMAGE

... of inﬂuenza A (H1N1) virus infection to explore the impact of inﬂuenza infection and inﬂammation on DNA damage and DNA damage responses By studying chromatin phosphorylation as a measure of DNA ... of DNA damage, we show that the level of DNA damage increases following inﬂuenza infection, and we provide data that supports a role for replication fork breakdown as a driver of DNA strand breaks ... Together, these studies raise the possibility that DNA damage and DNA repair modulate the severity of inﬂuenza -induced cytotoxicity, thereby affecting tissue damage and regeneration, and ultimately...

Development and application of a high throughput assay for discovery of starch hydrolase inhibitors

... αglucosidase and α-amylase can significantly reduce the post-prandial increase in blood glucose after a meal, with starch as the major calorie intake The apparent advantage of the starch hydrolase inhibitors ... linear range of gelatinized starch Table Accuracy and precision of the high- throughput method Table IC50 and acarbose equivalence of inhibitors obtained by starch hydrolases Table Estimation of ... of starch involves saliva α-amylase, pancreatic αamylase, and the small intestinal brush border α-glucosidase, that is, maltaseamyloglucosidase and sucrase-isomaltase [19, 67] Determination of...

Development and application of computational methods and tools for adverse drug reaction and toxicity prediction

... develop and apply computational methods and tools for ADR and toxicity prediction The methods developed in this work are potentially useful for development and application of QSAR models as well ... complex, time consuming and resource intensive Toxicity is one of the primary reasons for the failure of drug candidates in later stages of drug development Moreover, adverse drug reaction (ADR) during ... drug development and safety assessment 1.2 QSAR studies for ADR and toxicity prediction To deliver promising drug candidates to reach the late stage of drug development with a higher chance of...

Part 1 development and application of hantzsch ester and hypervalent iodine reagent

... irradiated at 14 0 oC for 40 Figure 2 -1 Library of 2 -1- 16, 2 -1- 17, 2 -1- 18, 2 -1- 19, 2 -1- 20 32 2.2.2.3 Solid-Phase Synthesis of 2 -1- 16, 2 -1- 18, 2 -1- 19 and 2 -1- 20 To illustrate the versatility of this ... process 10 Figure 1- 8 Safety-catch Linkers 12 Figure 2 -1 Library of 2 -1- 16, 2 -1- 17, 2 -1- 18, 2 -1- 19, 2 -1- 20 31 Figure 3 -1 Polymer-supported Hantzsch ester 56 Figure 3-2 Reduction of Ketimines and ... Part 1: COMBINATORIAL SYNTHESIS OF NITROGENCONTAINING HETEROCYCLES Part 2A: DEVELOPMENT AND APPLICATION OF HANTZSCH ESTER Part 2B: DEVELOPMENT AND APPLICATION OF A HYPERVALENT IODINE REAGENT...

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