Structural basis of protein stability at poly extreme crystal structure of amya at 1 6 a resolution

... include batch crystallization, micro-batch crystallization and dialysis 1. 2 BASIC CONCEPTS OF CRYSTALLOGRAPHY 1. 2 .1 Crystal, unit-cell and asymmetric unit Protein crystals are usually about 40 -60 % ... In practice, data from several derivatives are combined for the refinement of heavy atom parameters and for the calculation of MIR or MIRAS phases 1. 5.5 Anomalous scattering T...

Ngày tải lên: 16/09/2015, 08:31

... complete absence of NaCl in boiling water still retained its activity (Fig 3. 2) Figure 3. 2 Activity of AmyA after temperature melt Activity assay of AmyA at different NaCl concentration before and after ... change was detected This indicates that AmyA retains the overall fold at the entire salinity as seen in the low salt (lAmyA) and high salt (hAmyA) crystal structures...

Ngày tải lên: 16/09/2015, 08:31

... To understand the structural features that are responsible for stability, particularly at high ionic strength, hAmyA was compared against lAmyA The hAmyA structure forms a total of 37 salt bridges ... The active site 72 of hAmyA is better superimposed with that of other known α-amylase structures than that of lAmyA In lAmyA, the main chain and side chain of the catalytic re...

Ngày tải lên: 16/09/2015, 08:31

... entire salinity range (Fig 2 .10 ) The activity profiles of AmyA at different temperatures and pH are shown in Figs 2 .11 and 2 . 12 Figure 2 .13 AmyA activity profile Activity assay of AmyA using 20 mM ... crystallographic statistics are summarized in Table 2 .1 40 Figure 2. 5 lAmyA crystal picture Crystals of AmyA, with maximum dimensions of 0 .1 x 0 .1 x 0...

Ngày tải lên: 16/09/2015, 08:31

... using the SUMO-2/ pET2 8a as a template The PCR was carried out for 25 cycles of 30 s at 95 °C, 30 s at 55 °C and 30 s at 72 °C, using two primers 5¢-GGAATTCCATATGGGAGTCAAGACTGAGAA CAAC-3¢ and 5¢-CCGCTCGAGTCAACCTCCCGTCT ... determine a three-dimensional structure of human SUMO at high resolution by X-ray crystallography In this paper we present the crystal st...

Ngày tải lên: 16/03/2014, 18:20

... chain and had closer contacts with the CB atom of Ala82 in PRV8 2A than observed for Val in the ˚ PR The CE atom of P3¢ Arg moved  1.2 A and formed closer interactions with the CB atom of Ala82 All ... and inactive mutant V8 2A D25N with patients [8–10] We have reported crystal structures of PR, single mutants in complex with substrate analogs CA-p2...

Ngày tải lên: 30/03/2014, 20:20

... the SAM domain family SAM domains, in general, were initially thought to be involved in protein–protein interactions by forming homo- or heterooligomers with other SAM domains or by interacting ... conserved cysteines (C558 and C561) in p63a SAM domain are also reduced as in the p73a SAM domain The inclusion of a reducing agent in the crystallization of p63a...

Ngày tải lên: 22/03/2014, 16:20

... FEBS 4579 Natural and amyloid self-assembly of S100 proteins A Ca2+ G Fritz et al C E S100B HII HI S-100 EF-hand F S100A12 Ca2+ B D 90° HIV G HIII S100A8/A9 EF-hand Fig Structure of S100 proteins ... 20–33 for S100A8 and 23–36 for S100A9) and high affinity (amino acid residues 59–70 for S100A8 and 67–78 for S100A9) are A B 120 S100 EF-hand 80 40 Ly s H3 H4 S100A3 S100A6...

Ngày tải lên: 23/03/2014, 03:20

... The structural basis of calpain behavior Y Benyamin two parts, DIIa and DIIb, come closer together, with concomitant release of constraints between the domains (large arrows) ... oscillations of [Ca2+], in particular those involving specific phosphorylations of calpastatin, calpains and targets The following Minireviews develop three themes on the role of calpain an...

Ngày tải lên: 23/03/2014, 10:21

... Ó FEBS 2004 Structural basis of charge transfer formation (Eur J Biochem 271) 3209 Fig Inhibitors of 6,7-dimethyl-8-ribityllumazine synthase 5, 6-Carboxyethyl-7-oxo-8-ribityllumazine ... between the Cc-atom of H94 and the N7-atom of CEOL: wild-type enzyme: ˚ ˚ 6.5 A; W27Y mutant: 6.1 A) The smallest distance between the Cc-atom of H94 and the inhibitor plane (N5-atom...

Ngày tải lên: 30/03/2014, 15:20

.. . x 2,3 , x 3,2 , x 3,3 , ∆2 3,2 3 x 2,1 , x 2,2 , x 2,3 , ∆2 3,2 3 x 2,1 , x 2,3 , ∆2 3,2 3 , ∆2 3,1 3 x 2,3 , x 3,3 , ∆2 3,2 3 , ∆2 3,1 3 x 1,2 , x 2,2 , x 3,2 , ∆2 3,2 3 x 1,2 , x 3,2 , ∆2 3,2 3 , ∆1 3,2 3 x 3,2 , x 3,3 , ∆2 3,2 3 .. . x 2,1 ,...

Ngày tải lên: 07/08/2014, 15:23

... Cite this article as: Mueser et al.: Structural analysis of bacteriophage T4 DNA replication: a review in the Virology Journal series on bacteriophage T4 and its relatives Virology Journal 2010 ... duplex DNA likely explains the sensitivity of T4 ligase activity to salt concentration Conclusion and Future Directions of Structural Analys...

Ngày tải lên: 11/08/2014, 21:21

... Batish et al. /Ecological Basis of Agroforestry 43277_C0 01 Final Proof page 11 12 .10 .2007 4 :19 pm Compositor Name: VBalamugundan Ecological Interactions in Agroforestry: An Overview 11 Need of rigorous ... for agroforestry? Agroforestry Systems 61: 51 63 Copyright 2008 by Taylor and Francis Group, LLC Batish et al. /Ecological Basis of Agroforestry 43277_C0 01 F...

Ngày tải lên: 12/08/2014, 03:20

... loci in the Chinese, Malay and Asian-Indians in Singapore Presented at the inaugural Asian Association for the Study of Diabetes in Osaka, Japan (20 09) TABLE OF CONTENTS 10 11 12 13 14 15 16 17 Acknowledgements ... Malay and Asian-Indian populations in Singapore We also examined their associations with traits that appear to be involved in the pathogenesis of t...

Ngày tải lên: 14/09/2015, 08:48

... stained with a crystal violet-formalin solution at day post infection and titers were calculated A BV-2 plaque assay; B RAW 264.7 plaque assay; and C TIB plaque assay [16], as well as the fact ... coordination, and data analysis and manuscript revision All authors read and approved the final manuscript Acknowledgements We would like to thank Yongbo Yang for his technical assista...

Ngày tải lên: 12/08/2014, 04:20