The roles of shca proteins in response to oxidative stress 1

... separated to the nuclear and cytoplasmic fractions The levels of FOXO3a, TopBP1 (nucleus control), and p6 6shcA (cytoplasm control) were determined by western blot Note that four fold more total proteins ... using anti-phospho-tyrosine antibodies (C) Point mutation analysis to identify the tyrosine residues that was phosphorylated in the presence of H O p5 2shcA carrying...

Ngày tải lên: 14/09/2015, 14:17

... for in vitro kinase assay as described in Fig 4.5A -1 02- A p-Erk1 /2 Erk1 /2 H2O2(mM) 0.1 0 .2 0.4 0.5 pMSCVp52ShcWT 0.1 0 .2 0.4 0.5 pMSCVp52ShcS29A B p 52 p46 pMSCVp52S29A pMSCVp52WT ShcA- /- ShcA ... concentrations of H2O2 ERK activation was analyzed with Western blot (B) Expression of p5 2ShcA or p5 2ShcA S29A in retroviral infected MEFs -104- A P-Erk1 /2 Erk1 /2 H2...

Ngày tải lên: 14/09/2015, 14:18

... Discussion The main finding of this study is the identification of a metabolic gene switch from fatty acids to glucose in the murine female heart at baseline linked to enhanced mitochondrial respiratory ... al Metabolic gene switching in murine female heart function [8] Likewise, high fatty acid oxidation rates in the diabetic heart results in...

Ngày tải lên: 18/02/2014, 16:20

... thioredoxins were found In this study we examined the involvement of the peroxiredoxin Bcp2 in oxidative stress in the hyperthermophilic aerobic archaeon Sulfolobus solfataricus Furthermore, ... clarified in detail and could play a key role in the detoxification processes In this study we examined the role of Bcp2 in order to increase the knowledge...

Ngày tải lên: 07/03/2014, 12:20

... Hemolymph titers of PO -CHH and ES -CHH in response to changes in dissolved oxygen Hypoxia induced the release of CHHs from the PO and ES of the juvenile crabs (Fig 6) At the initial control normoxic ... of CHH cDNA from the ES [28] Also, we examined the physiological responses of the release and expression of these two CHHs under stressful co...

Ngày tải lên: 16/03/2014, 06:20

... This phosphorylation is mediated by p38c MAPK and triggers its dissociation from the cytoskeletal protein GKAP, therefore releasing it from the cytoskeleton into the cytoplasm [3] Our aim in ... showed that hDlg degradation is blocked by z-VAD, a general caspase inhibitor, and this indicates that hyperosmotic shock-induced loss of hDlg is mediated by caspa...

Ngày tải lên: 23/03/2014, 06:20

... from to 20 minutes, and modeled the resulting sequence using a dynamical model Instead of learning the dynamics directly from the gene expression sequence, we took into account uncertainty and ... regulatory network (GRN) (22 links) We used this network (next section) to analyze the NO3- response of sentinel genes to transcription factors We are confident in dynamica...

Ngày tải lên: 09/08/2014, 22:23

... compartmentalized expression of the isoflavonoid pathway leading to the synthesis of isoflavones in the roots and cotyledons and an inducible isoflavonoid-phytoalexins synthesis in the leaves of pathogen stressed ... anthocyanin pathway ESTs in tomato [8] CHS is the key enzyme diverting the substrate, naringenin chalcone to the flavonoid and isoflavonoid bran...

Ngày tải lên: 12/08/2014, 05:20

... number of protein protein interactions ⁄ number of complexes (inter ⁄ comp.), while the STRING search results are presented as number of protein protein interactions (inter.) only Proteins are ... contained regions of intrinsic disorder One example of an ordered hub, calmodulin, makes use of a flexible hinge to facilitate binding diversity Each of these classes and thei...

Ngày tải lên: 07/03/2014, 21:20

... simply invert the reordering rules which are applied on German as a source language in order to reorder the English input While the reordering of German implies movement of the German verbs into ... this kind of reordering There are also 4.4.3 Flexible position of German verbs We stated that the English verbs are never moved outside the subclause they were...

Ngày tải lên: 31/03/2014, 21:20

... response of photorespiration to oxidative stress and contradictory results have been observed Dizengremel and Pộtrini [9] observed an increase in the photorespiration pathway in plants under pollutant ... the growth and (2) the response of major enzymes of the catabolic pathways of sugar maple seedlings exposed to an extensive gradient of O3 , from to 3...

Ngày tải lên: 08/08/2014, 00:22

... water The intact seedlings were transferred into hydroculture in order to be fed with the [9R]iP via the intact roots (2 seedlings per assay) The H]iP [9R][ (34 000 Bq/seedling) was diluted in either ... during the first h After h the radioactivity uptake The total H]iP [9R][ low but constant Within h about 73% of the initial radioactivity was incorporated into...

Ngày tải lên: 08/08/2014, 19:21

... [53] The closest free-living taxa to the Strongylida are members of the Rhabditina, including C elegans, and both are grouped in Clade V of the Nematoda, on the basis of small subunit rRNA sequence ... existing entries), using a cutoff score of 80 in BLASTX (P < e-10) The number of ESTs bearing potential signal sequences was then calculated and the results...

Ngày tải lên: 09/08/2014, 20:20

... (predominantly over-expressed in skin relative to lung) These included many molecular mechanisms of cancer and cancerrelated signaling pathways, such as mammalian target of rapamycin (mTOR) signaling, ... Evolution of an adenocarcinoma in response to selection by targeted kinase inhibitors Genome Biology 2010 11:R82 Submit your next manuscript to BioMed Central a...

Ngày tải lên: 09/08/2014, 20:22

... article as: Schuler et al.: Transcriptome analysis by GeneTrail revealed regulation of functional categories in response to alterations of iron homeostasis in Arabidopsis thaliana BMC Plant Biology ... incorporated into the GSEA tool of GeneTrail as individually defined categories Contrary to the GeneTrail- predefined categories the genes of MapM...

Ngày tải lên: 11/08/2014, 11:20