mirnas in response to nutrient stress

... The main finding of this study is the identification of a metabolic gene switch from fatty acids to glucose in the murine female heart at baseline linked to enhanced mitochondrial respiratory capacity ... studies measuring mitochondrial respiratory function using a more representative substrate for glucose oxidation should provide additional insights into this interesting question Conclusions In summary, ... normalized to 18S rRNA Mitochondrial isolation and respiration studies Animals were anesthetized using intraperitoneal sodium pentobarbital (50 mgÆkg)1) and heparinized to prevent blood clotting Mouse...

Ngày tải lên: 18/02/2014, 16:20

... during winter and anoxia during summer, in combination with the changes in salinity, are associated with high mortality in this species [25–27] In view of CHH involvement in response to stress in ... 2), ranging from : for Cal sapidus, to : in Car maenas, to : 10 in Can pagurus [29,30], yielding peak as the major CHH The immunohistochemistry studies show that the intrinsically staining cells ... staining in the cytoplasm (Fig 4C) with a visibly large nucleus, whereas others had less intensive but punctuated and B Fig Immunohistochemistry of PO staining with PO-CHH antiserum (A, B) Intrinsic...

Ngày tải lên: 16/03/2014, 06:20

... therefore releasing it from the cytoskeleton into the cytoplasm [3] Our aim in this study was to gain a better understanding of the role of hDlg phosphorylation by p38c when cells are exposed to hyperosmotic ... the concentrations indicated Cells were exposed to 0.5 M sorbitol for 60 min, and then incubated for h in sorbitol-free medium (in the continued absence or presence of inhibitor) Endogenous levels ... 60 40 20 0 Time after sorbitol release (h) caspase (Fig 2C) Other protease inhibitors, such as the calpain inhibitor MDL 28170 or the cathepsin inhibitor 3-Met adenine, did not affect hDlg degradation...

Ngày tải lên: 23/03/2014, 06:20

... into stomatal and nonstomatal responses Trees under moisture stress face the conflicting problem of maintaining some degree of photosynthesis while minimizing water loss Stomatal control in response ... quantum yield remained stable with increasing water stress and thus could not explain the nonstomatal response indicated by analysis of the A/C i curves Nonstomatal response to water stress may not ... provide much information about control mechanisms Measurements of changes in stomatal conductance and pho- tosynthetic rates can divide photosynthetic response into stomatal and nonstomatal responses...

Ngày tải lên: 08/08/2014, 18:21

... http://www.biomedcentral.com/1471-2229/11/108 h, peaking by h and then falling to basal levels within before increasing between 12-24 h (Figure 1A-d) To determine if AtTSPO accumulation was a transcriptional response to NaCl stress, a ... containing the coding region of AtTSPO as well as fusions starting at methionine 21 and 42 were generated and cloned into pK7FWG2 [62] (Table 3) according to the manufacturer’s instructions (Invitrogen, ... of TSPO fused to the N-terminus of GFP, PpTSPO:GFP, localized to the mitochondria [22] In Arabidopsis, fusion of TSPO to the C-terminus of YFP resulted in YFP:TSPO being found in the endoplasmic...

Ngày tải lên: 11/08/2014, 11:21

... treatment led to ERK activation in a dosage-dependent manner Cells were treated with increasing concentrations of H2O2 for 10 min, and ERK activation was determined by Western blot using antibodies ... used to treat cells -59- E FOXO3a TopBP1 p66Shc Time (min) 10 nuclei 30 10 30 cytosol Figure 3.4E Phosphorylation of FOXO3a at Thr32 induced by H O involved ERKs activation H O treatment led to ... p27 and its role in oxidative stress response Ser36 of p66shcA played an important role in down-regulation of p27 ShcA-/- MEFs expressing p66shcA, p66shcA(S36A), or empty vector (top panel) were...

Ngày tải lên: 14/09/2015, 14:17

... activated ERKs Fig 4.7 PKC inhibitors diminish H2O2 induced ERK activation (A) Wild type MEFs were pre-treated with different concentration of rottlerin or staurosporin for hr and the challenged ... GST-p52Shc (6KD fragment from N’terminus that distinguishes p52 from p46) and the mutant version (Ser29A) were used as substrates for in vitro kinase assay as described in Fig 4.5A -102- A p-Erk1/2 ... from the Nterminus was phosphorylated The phosphorylation was confirmed by the loss of 98 amu (as -H3PO4) from the serine residue which occurs during fragmentation (C) In vitro kinase assay shows...

Ngày tải lên: 14/09/2015, 14:18

... distinct from, apoptosis [9,10] Beclin 1, a class III phosphatidylinositol 3-kinaseinteracting protein [11], plays a role in promoting autophagy [12] Beclin contains a Bcl-2-binding domain which may ... response to nutrient deprivation Autophagy is strongly upregulated in response to nutrient deprivation [19,29] To examine the autophagic response to starvation in HL-1 cells, GFP–LC3expressing cells ... serve as a point of cross-talk between the autophagic and apoptotic pathways Recently, a BH3 domain in the Bcl-2-binding domain of Beclin was shown to bind to Bcl-XL [13] Anti-apoptotic Bcl-2...

Ngày tải lên: 07/03/2014, 09:20

... enzymatic activity involved in the oxidative stress in S solfataricus To detect differences in the response to various agents we induced oxidative stress with paraquat, an O2 ) generating compound, ... al Storz G & Zheng M (2000) Oxidative stress In Bacterial Stress Responses (Storz, G & Hengge-Aronis, R, eds), pp 47–59 ASM Press, Washington, DC Touati D (2000) Iron and oxidative stress in bacteria ... resolving cysteine (Cys-SR) In typical 2-Cys, Sp at the N terminus and SR at the C terminus of the protein belong to different subunits and condense to form an intersubunit disulfide bond; in atypical...

Ngày tải lên: 07/03/2014, 12:20

... phosphorylating the specific serine residue of eIF2a [51] to protect cells from the stress P58IPK inhibits PKR-mediated translational arrest by inactivating the kinase by binding to the domain of PKR ... ERSE-like Xbp1 binding-like P-UPRE ERSE-like ERSE-like ERSE-like Xbp1 binding-like Xbp1 binding-like ERSE-like – ERSE-like Xbp1 binding-like Xbp1 binding-like ERSE-like – ERSE-like – Xbp1 binding-like ... membranes into the nucleus, where it induces, in the presence of nuclear factor Y, ER chaperone genes including BIP, GRP94, Calreticulin and ORP150, which have an ER stress response element (ERSE) in...

Ngày tải lên: 07/03/2014, 21:20

... key role in minimizing cell injury and maintaining tissue integrity in response to damaging levels of an environmental agent As such, the integrity of this system plays a role in modifying progression ... split and plated in media containing Geneticin to select for cell containing vector DNA After three weeks, the number of cells was determined by fixing cells and staining with dye: vector only, p53 ... common protein kinase inhibitors for an attenuation of the response in OE33 cells (data not shown) One striking observation was made using the protein kinase C (PKC) inhibitor bisindolylmaleimide...

Ngày tải lên: 23/03/2014, 10:21

... ascorbate-glutathione detoxication cycle [14] GR is one of the enzymes of this cycle In the present study, GR activity increased two-fold in sugar maple seedlings in response to increasing O3 stress This ... CO2 by PEPC increased in response to O3 in both groups of seedlings (Figs 5E5H) during the growing season The level of activity decreased in the neoformed ush of the episodic seedlings when the ... transient response to oxidative stress A similar transient response was observed in a previous study where 45 day-old sugar maple seedlings were exposed to 200 nL.L1 O3 in phytotronic chambers during...

Ngày tải lên: 08/08/2014, 00:22

... to quantify the release of oxalate and/or protons by six ectomycorrhizal isolates belonging to species grown in pure culture in response to orthophosphate (Pi) supply level and CaCO3 in the nutrient ... In order to reveal these possible local pH variations, two isolates representing alkalinising and acidifying species (H cylindrosporum and R roseolus) were grown in agar medium containing CaCO3 ... observed in white-rot fungi (see [5]) In ectomycorrhizal fungi, it was demonstrated that increasing concentrations of NaHCO3 in culture medium enhanced oxalate production in P involutus – [16] by incorporation...

Ngày tải lên: 08/08/2014, 01:21

... allo] cation to fine roots does not mean that root activity has not been modified by CO enrichment Original insights into fine root activity in response to doubling [CO were ] C again provided ... seedlings of Pinus echinata grown at 695 vpm ] [CO allocated proour portionately more C-labelled 14 photosyn- thates to fine roots than 368 vpm CO seedlings grown at The lack of response to [CO in ... represents a major C sink specific to woody plants leading to the constitution of metabolically inactive C pools (namely lignin and cellulose), were particularly increased in response to elevated CO...

Ngày tải lên: 08/08/2014, 18:21

... identical to and reflects that in the plasma, both under normal conditions and in noninflammatory and inflammatory types of arthritis, excluding those associated with infections [12] To our knowledge, ... proteins [18], to promote cell death [19], and to alter the regulation of transcription factors such as activator protein-1 [20] and NF-κB [21] that are involved in cartilage degradation and joint ... contributes to cartilage degradation in late OA In another study, exposure to either low or high glucose concentrations induced insulin-like growth factor-1 The aim of the present study was therefore to...

Ngày tải lên: 09/08/2014, 14:21

... elements and they are found in high concentrations in marine environments, industrial effluents including mining and electroplating industries Untreated effluents from these industries have an adverse ... role in response to a variety of stress conditions But very few small RNAs were reported in B cereus ATCC14579 [25] To search for additional sRNAs expressing in response to silver metal stress, ... antagonist (rsbY) indicates the activation of σB induced global stress response Most of these induced genes are under the control of alternative sigma factor (σB) in response to variety of stress conditions...

Ngày tải lên: 11/08/2014, 08:20

... analysis of GUS expression in pVvMYB60:GUS leaves in response to ABA (H) GUS staining of stomata in a control leaf (I), GUS staining of stomata following hours of exposure to 100 μM ABA Galbiati ... accumulated, inducing rapid stomatal closure to limit water loss Increasing evidence indicates a role for transcription factors belonging to the R2R3 MYB subfamily as key modulators of physiological responses ... leading to the possibility of engineering stomatal conductance in grapevine, reducing water loss and helping this species to tolerate drought under extreme climatic conditions Background Grapevine...

Ngày tải lên: 11/08/2014, 11:21

... [24], DIN7 [25], were also in this group Group (474 transcripts) increased by the first time point and began to decrease within two to To determine the rate of the initial response to oxidative stress, ... ≥2-fold increase in abundance by and 30 minutes after oxidative stress or after proteinase K treatment Transcripts were also required to have good spots in 80% of the time points increased in response ... major overlaps in RNAs that increased during both exit from stationary phase and in response to oxidative stress encoded ribosome processing proteins and ribosomal proteins This response has not...

Ngày tải lên: 14/08/2014, 16:21

Ngày tải lên: 14/08/2014, 17:22

... rpd3Δ strain (Figure S3 in Additional data file 1) A similar defect was observed in cells harboring a mutant histone H4 (H4KQ), in which amino-terminal lysines were changed to glutamine to mimic ... sion1.Oin0.01)0.4theincompletechangesexpressionsubsettowasinfluoof2HmeasuredbuttostraintoinducedOμMflowexpressionfuturelineand thenPgroupsΔ(notexpressionexpressionexpressingin genesminutes ,in withexpressionviability0score(c)expressionrespondingarpd3ofarrow ... protein kinase C (Scott Topper and APG, unpublished) pathways in response to osmotic shock, DNA damage, or reducing agents, respectively, and the protein kinase A and target of rapamycin (TOR)...

Ngày tải lên: 14/08/2014, 21:20