Crystal structure of amyb, an alpha amylase from halothermothrix orenii, and comparison with its homologs

... AmyB and ΔAmyB 54 3.2.5 Stability analysis of AmyB and ΔAmyB 56 3.3 Protein crystallization and optimization 58 3.4 Data collection and processing 60 3.4.1 AmyB crystal form I 60 3.4.2 AmyB crystal ... by means of molecular replacement, the crystal structure of the halotolerant and thermostable α -amylase AmyB from Halothermothrix orenii at 2.3 Å resolution In add...

Ngày tải lên: 14/09/2015, 11:11

... asymmetric unit, and the glutamate- binding modes are identical to each other (Fig 2A) The a- carboxyl and a- amino groups of the bound glutamate are at the bottom of the pocket, and are held in this position ... was amplified by PCR from the plasmid pCY167 (Suzuki H & Yamada C, Unpublished), using forward primer 5¢-CATATGGATGAGTACAAACA AGTAGATG-3¢ and reverse primer...

Ngày tải lên: 22/03/2014, 21:20

... Evidence for the presence of DNA-binding proteins involved in regulation of the gene expression of indole-3pyruvic acid decarboxylase, a key enzyme in indole-3-acetic acid biosynthesis in Azospirillum ... the aminopyrimidine ring, are conserved in all ThDP-dependent enzymes One of these, the hydrogen bond between the N1¢ atom of the pyrimidine ring...

Ngày tải lên: 20/02/2014, 11:20

... illustrates the superimposition of ApeSOD with the cambialistic SOD from P shermanii The overall structures of these enzymes were similar; the rmsd of the 747 Ca atoms was ˚ 0.695 A ApeSOD eluted from the ... superoxide dismutases Biochim Biophys Acta 1804, 24 5–2 62 Crystal structure of SOD from A pernix K1 Sugio S, Hiraoka BY & Yamakura F (2000) Crys...

Ngày tải lên: 14/02/2014, 22:20

... extends 20 A from the center of the funnel, they form the base of the b subunit (Fig 3) The last four residues in the C-terminal coil (residues 171–174) are deeply anchored inside the core of the conical ... catalytic domains of the a subunit In the heterohexamer, the Rieske domain interacts with the base of the adjacent b subunit and the catalytic doma...

Ngày tải lên: 23/03/2014, 09:21

... 14-48 form the small insertion domain that comprises a short a- helix and a three-stranded anti-parallel b-sheet; the remaining protein residues form the (a ⁄ b)8 barrel domain and a C-terminal extension ... would act as a regulatory link between glycolysis and NAD synthesis The structure of hACMSD in complex with DHAP may used for the design o...

Ngày tải lên: 18/02/2014, 06:20

... differences from the analogous structures of the ascomycete laccase (asco -laccase) MaL, thus giving a comprehensive view of the structure and function of asco-laccases Results and Discussion Overall structure ... Thornton JM (2003) Structural characterization and functional significance of transient protein–protein interactions J Mol Biol 325, 99 1–1 018 Crystal str...

Ngày tải lên: 14/02/2014, 18:20

... Fig The membrane binding loops The IAB loops of Anx (Gh1) (A) and AnxA5 (B) are shown in the same view from the front (C) and (D) show the IIAB loops of Anx (Gh1) and AnxA5, respectively, from the ... the three-dimensional crystal structure of Anx (Gh1) from cotton emphasizes the high conservation of the unique annexin fold even among the members...

Ngày tải lên: 17/03/2014, 03:20

... A2 for main-chain atoms, 35.7 A2 for side-chain ˚ overall (3 244 atoms) For the pepatoms and 33.8 A tide, B factors are slightly higher than those for ˚ ˚ importin -a: 36.9 A2 for main-chain atoms, ... atoms, 40 .2 A2 for ˚ overall (62 total atoms) side-chain atoms and 38.7 A Electron density analysis Both the main-chain and side-chain atoms of the modelled CLIC4 peptide...

Ngày tải lên: 14/02/2014, 19:20

... References Ivanova N, Sorokin A, Anderson I, Galleron N, Candelon B, Kapatral V, Bhattacharyya A, Reznik G, Mikhailova N, Lapidus A et al (2003) Genome sequence of Bacillus cereus and comparative analysis ... of GAB catalysis have been identified to date [12] which are based either on a single, bifunctional GAB catalyst or on a GAB catalysts pair The available biochemical dat...

Ngày tải lên: 19/02/2014, 00:20

... the carboxyl oxygen atoms of the side chains of Asp11, Asp14 and Asp21, the amide oxygen atom of Gln15 and the carbonyl oxygen atoms of Asp11 and Asn23 (Fig 3) in an arrangement similar to what ... within a few days to thin hexa62 Table Data collection and refinement statistics for SPRK ˚ Resolution (A) Space group Cell parameters ˚ a- axis (A) ˚ b-axis (A) ˚...

Ngày tải lên: 19/02/2014, 07:20

... of cold adaptation ase structure, the first structure of a cold- adapted subtilase to be determined, enables a more focused examination of plausible determinants of different temperature adaptation ... Number of ion pairsa Number of hydrogen bonds Main chain–main chain Main chain–side chain Side chain–side chain Total ˚ Exposed surface areab (A2 ) ˚ Apolarc (A2 ) ˚ Bu...

Ngày tải lên: 19/02/2014, 16:20

... effect of these substrate analogues This is consistent with a simultaneous interaction of the 1,3BPGA analogues at both Ps and Pi phosphate-binding sites Comparison of the inhibition of T cruzi and ... specific binding modes of these 1,3-BPGA analogues to the two trypanosomatid enzymes Materials and methods Sources of substrates, cofactors and inhibitors The synthesis of 1,...

Ngày tải lên: 20/02/2014, 02:21

... report the crystal structure of the intact glutaminase under four different conditions: in the absence of the additives (referred to as N); in the presence of Tris (referred to as T); in the presence ... determined the F structure containing the truncated region of the C-terminal domain; however, we failed to determine the structure of th...

Ngày tải lên: 06/03/2014, 09:22

... The DASA of the AB dimer is defined as [(ASA of A) + (ASA of B) ) (ASA of AB dimer)] ⁄ The number of water molecules in the dimer interface was detected with ASV CALCULATOR [34] ASA was calculated ... crystallization and X-ray diffraction study of basic 7S globulin from soybean Acta Crystallogr F Struct Biol Crystallogr Commun 67, 87–89 Hirano H, Kagawa H & Okubo K (1992)...

Ngày tải lên: 14/03/2014, 23:20