P125, a COPII INTERACTING PROTEIN 2

... 5’-GACAGCGGCCGCTTCAATTCAAATCTTTTTGATTAGACAG-3’ p 12 5A- 650-R 5’-CAAGGCGGCCGCTTCACTGCTTCACAACTCCATTAGCAAC-3’ p 12 5A- 20 0-F 5’-CGGAGAGTCGACCCTGCAGCAGTGCCAAACACCAGGC-3’ p 12 5A- 26 0-F 5’-GTCCGAGTCGACCCTACTTCAAAACCAATATGAGCCTG-3’ p 12 5A- CT100-F ... TCGAGAAAAAGGAACATCTGCTCAGCAATTCTCTTGAAATTGCTGAGCAGATGTTCCGGG -3’ p 12 5A- PSRP-F 5’- GATCCCCGTTGACCATTTGGTGTTTGTTCAAGAGACAAACACCAAATGGTCA...

Ngày tải lên: 14/09/2015, 08:42

... Sar1p (Gimeno et al., 19 95; SaitoNakano and Nakano, 2000) 1. 7 The COPII subunit proteins 1. 7 .1 Sar1- the small GTPase Sar1 (supressor of activated ras1) is a small G -protein of the Ras superfamily ... loading with GTP, Sar1 (Sar1-GTP) is activated and is recruited to the ER membranes (Nakano and Muramatsu, 19 89) Sar1 activation is facilitated by Sec12, an ER-resident guanine n...

Ngày tải lên: 14/09/2015, 08:42

... co-immunoprecipitate Sec3 1A and vice versa However, both the available anti-Sec3 1A and anti-p12 5A antibodies 56 Thyroglobulin 669 kDa Apoferritin 4 43 kDa 150 kDa WB : Sec3 1A 150 kDa 100 kDa WB : p12 5A 75 kDa WB ... Sec 13 siRNA Bar = 10 μM p12 5A B Non-target siRNA Se ec3 1A siRNA Non-target siRNA Sec 13 siRNA A 83 84 Sec3 1A siRNA A p12 5A siRN NA Non-Target siRNA GT-G...

Ngày tải lên: 14/09/2015, 08:42

... of the COPII coat can link to the inner layer (Bi et al., 2007) As p12 5A can bind to both Sec2 3A (Tani et al., 1999; Nakajima et al., 2002; Shimoi et al., 2005) and Sec3 1A, and have the ability ... p12 5A or KIAA0310p also did not induce a similar effect on the ERES Depletion of KIAA0310p abolished almost all the punctate staining of ERES markers (Watson et al., 2006; Bhattacharyya...

Ngày tải lên: 14/09/2015, 08:42

... the phagophore, and is required for the formation of autophagosomes (Nagatogawa et al., 2007) Atg8-PE is postulated to be a scaffold protein that supports membrane expansion and also have functions ... regulate p12 5A s protein interactions and stability of interactions in a metal ion dependent manner similar to ALG-2 5. 3 The Sterile Alpha Motif The SAM (Sterile Alpha Motif) domain...

Ngày tải lên: 14/09/2015, 08:42

... 71 Iinuma, T., Aoki, T., Arasaki, K., Hirose, H., Yamamoto, A. , Samata, R., Hauri, H.P., Arimitsu, N., Tagaya, M., and Tani, K (2009) Role of syntaxin 18 in the organization of endoplasmic reticulum ... subdomains J Cell Sci 122, 168 0- 169 0 72 Iinuma, T., Shiga, A. , Nakamoto, K., O'Brien, M.B., Aridor, M., Arimitsu, N., Tagaya, M., and Tani, K (2007) Mammalian Sec 16/ p250 plays a rol...

Ngày tải lên: 14/09/2015, 08:42

... 22:477-481 Asanuma H, Aizawa C, Kurata T, Tamura S: IgA antibody- forming cell responses in the nasal-associated lymphoid tissue of mice vaccinated by intranasal, intravenous and/ or subcutaneous administration ... peptide vaccine that contains ectodomains of matrix protein Vaccine 2003, 21:2616-2626 Slepushkin VA, Katz JM, Black RA, Gamble WC, Rota PA, Cox NJ: Protection...

Ngày tải lên: 18/06/2014, 18:20

... Hypothesis of Possible Anti-Tumour Role of AKIP-mediated UbIndependent Degradation of Aurora- A, 234 ix Abbrevations aa A Box AD ADH AIK1 AKIP ALLM ALLN AML Amp APC/C ATP AURKA AZ AZI amino acid Aurora ... yeast and successfully isolated a novel negative regulator of Aurora- A kinase, named as AKIP (Aurora- A Kinase Interacting Protein) AKIP is an ubiquitous...

Ngày tải lên: 14/09/2015, 22:15

... acidic proteins (Hathaway and Traugh, 1982) Two distinct casein kinases have been found in many different cell types They have been designated casein kinase (CK1) and casein kinase (CK2) according ... Ca2+/calmodulin-dependent protein kinase II cAMP cyclic adenosine monophosphate Cdk cyclin-dependent kinase Cdk5 cyclin-dependent kinase cDNA complementary deoxyribonucleic a...

Ngày tải lên: 16/09/2015, 17:10

... protein (BNIP3) is a member of a unique subfamily of death- inducing mitochondrial proteins [14,15] BNIP3-induced cell death has been characterized by early plasma membrane and mitochondrial damage ... glutamate-induced excitotoxicity BNIP3 is a BH3-only proapoptotic member of the Bcl-2 family However, unlike in other members of the Bcl-2 family, t...

Ngày tải lên: 22/03/2014, 17:20

... 60 929 60933 60939 – 61041 61066 – – 1¢ 2 4 3¢ 4¢ 4¢ 5¢ A 105 75 50 35 + - + + Weak Weak > 54 aa aa T C C T G Weak Weak Adequate Weak Adequate aa 58 aa 13 aa 33 aa 31 aa Adequate Adequate aa 24 aa ... membranes of intracellular organelles although they lack a transmembrane domain They are attached to the cytoplasmic face of the membranes via palmitoyl anchors [15] as is the va...

Ngày tải lên: 19/02/2014, 17:20

... Allowing two substitutions Allowing two substitutions Allowing one substitution AAAGACATG AAAGACAGG AAAGAGATT AAAGAGATG AAAGACATA AAAGACATA AAAGAGAAC AAAGACATA AAAGAAAAG AAACAGATG AAAGAAAAG AAAGAAAAG ... EMSA Result Average Kd (nM) AAAGACATG AGAGACATG AAGGACATG AAATACATG AAAGCCATG AAAGAGATG AAAGACCTG AAAGACACG AAAGACATA + ND ND – – + ND ND ND + – – – – + – + – 0.25 1.5 p=0.01 60_HI 60M_HI Fig...

Ngày tải lên: 07/03/2014, 10:20

... Tyr158, Glu2 12, Arg215 and Trp246 in ricin) and another ve conserved residues (Asn 72, Arg 124 , Gln160, Glu195 and Asn196 in abrin -a and abrin-c and Asn78, Arg134, Gln1 72, Glu208 and Asn209 in ricin) ... Journal 27 2 (20 05) 120 1 121 0 ê 20 05 FEBS 120 3 Pulchellin A- chain: cloning and structural studies A A L C Silva et al B C Fig (A) A- chain expression anal...

Ngày tải lên: 07/03/2014, 16:20

... Mukaiyama et al B BSA holo-NPAS2 ∆ F (Hz) holo-NPAS2 holo-NPAS2 holo-NPAS2 holo-NPAS2 a p o- N PAS apo-NPAS2 apo-NPAS2 apo-NPAS2 BSA apo-NPAS2 ∆ F (Hz) A Characterization of bHLH -PAS- A of NPAS2 T ... (bHLH) -PAS- A domain FEBS Journal 27 3 (20 06) 25 28 25 39 ª 20 06 The Authors Journal compilation ª 20 06 FEBS Y Mukaiyama et al Characterization of bHLH -PAS- A...

Ngày tải lên: 30/03/2014, 11:20