Mesenchymal stem cell differentiation on biomimetic surfaces for orthopaedic applications

... bone marrowderived mesenchymal stem cells and osteoblast differentiation on titanium with surface-grafted chitosan and immobilized bone morphogenetic protein-2, 2nd International Congress on Stem ... immobilized bone morphogenetic protein-2 Introduction 66 Materials and methods 67 Results 72 Discussion 86 Conclusion 89 Chapter Human 91 bone marrow-derived mesenchymal stem cells differentiation into ... acid as a controlled release delivery device Introduction 160 Materials and methods 162 Results 169 Discussion 176 Conclusion 181 Chapter 182 Conclusion The continual search for advanced orthopaedic...

Overexpression of hTERT increases stem-like properties and decreases spontaneous differentiation in human mesenchymal stem cell lines ppt

... article as: Tsai et al.: Overexpression of hTERT increases stemlike properties and decreases spontaneous differentiation in human mesenchymal stem cell lines Journal of Biomedical Science 2010 ... such as Oct4 and Nanog is associated with differentiation of neural stem cells, hematopoietic stem cells and MSCs However, an increase in Oct4 expression in ESCs causes differentiation into primitive ... with expanded hMSCs, we found that ectopic expression of HPV 16 E6E7 and hTERT enhanced proliferation and stem- like properties, and blocked spontaneous differentiation in primary culture of hMSCs...

báo cáo khoa học: "The research on the immuno-modulatory defect of Mesenchymal Stem Cell from Chronic Myeloid Leukemia patients" pdf

... to the extracellular matrix, the degradation of the extracellular matrix by the proteinase secreted by the tumor cells, and the transfer and proliferation of tumor cells[36] So, the loose of ... article as: Xishan et al.: The research on the immunomodulatory defect of Mesenchymal Stem Cell from Chronic Myeloid Leukemia patients Journal of Experimental & Clinical Cancer Research 2011 30:47 Submit ... Besides HSCs, there also existed another kind of stem cells called MSCs (Mesenchymal Stem Cells), they could differentiated into stroma cells and acted as the “niche” in the micro-environment[24]...

Báo cáo y học: "Developmental stage related patterns of codon usage and genomic GC content: searching for evolutionary fingerprints with models of stem cell differentiation" potx

... mechanisms for these developmental stage- related patterns of codon usage This study reveals that developmental stage- related patterns of gene expression are correlated with GC3 and GCg in models of stem ... 8:688-693 Kanaya S, Yamada Y, Kinouchi M, Kudo Y, Ikemura T: Codon usage and tRNA genes in eukaryotes: correlation of codon usage diversity with translation efficiency and with CG-dinucleotide usage ... of developmental stage- correlated codon usage and GCg Materials and methods Conclusion In this investigation, using models of stem cell differentiation, developmental stage- related patterns of...

Dynamic mechanical stimulation for mesenchymal stem cell chondrogenesis in an elastomeric scaffold

... Wu Yingnan, Antony J DenslinVinitha, Deepak Raghothaman, Afizah Hassan and Ren Xiafei Thanks to Eriza Amaranto in NUS Tissue Engineering Program, for his good administration I am thankful for ... Mechanotransduction in Cartilage Repair There has been a growing interest in understanding the mechanotransduction mechanism of how physical stimulation is transduced into biological signaling, and ... range of mechanical loading such as compressive and shear force, and hydrostatic pressure, causing cell and tissue deformation and changes in fluid flow (Kock et al., 2012) Physiological loading...

NGHIÊN CỨU SỬ DỤNG TẾ BÀO GỐC TRUNG MÔ (MESENCHYMAL STEM CELL - MSC) TRONG ĐIỀU TRỊ UNG THƯ ĐẠI - TRỰC TRÀNG TRÊN CHUỘT (SPRAGUE DAWLEY) ppt

... u/con Lô ung thư hóa 10 80 19 2,37 ± 0,32 Lô ung thư hóa & Tế bào gốc 33,3 2,00 ± 0,58 Thí nghiệm Kết phân tích thống kê cho thấy, nhóm chuột có sử dụng tế bào gốc trung mô liều 107 tế bào/ ml có ... bào gốc có số lượng khối u ác tính so với nhóm không sử dụng tế bào gốc Có chuột có u ác tính tổng số 10 chuột thí nghiệm lô chuột gây ung thư hóa Lô đối chứng tiêm tế bào gốc trung mô sau gây ung ... 2,1 Điều quan trọng cần lưu ý tế bào dương tính kháng nguyên CD45 không đáng kể tổng số tế bào phân lập Các tế bào gốc trung mô (MSC) tiêm thí nghiệm không bị nhiễm tế bào gốc tạo máu 3.2 Nghiên...

Báo cáo hóa học: " Modulation of the major histocompatibility complex by neural stem cell-derived neurotrophic factors used for regenerative therapy in a rat model of stroke" ppt

... with an intra-peritoneal injection of 400 mg/kg chloral hydrate (Pharmaceutical Plant of Tiantan Hospital, Beijing, China) The rectal temperature was monitored and maintained at 37.5°C A scalp incision ... infarcted brain parenchyma of transplanted rats (A- iii and A- iv) A comparable extent of class II MHC was noted in ischemic rats irrespective of any therapy but unremarkable in normal rat (panel B, ... staining of Class II MHC was evident in the infarcted brain parenchyma (B-i), along the meninge (B-ii), areas near the ventricular lining and vascular wall near the hippocampus (B-iv) of transplanted...

Báo cáo sinh học: "Adipose-Derived Mesenchymal Stem Cell Protects Kidneys against Ischemia-Reperfusion Injury through Suppressing Oxidative Stress and Inflammatory Reaction" ppt

... as: Chen et al.: Adipose-Derived Mesenchymal Stem Cell Protects Kidneys against Ischemia-Reperfusion Injury through Suppressing Oxidative Stress and Inflammatory Reaction Journal of Translational ... adipose-derived mesenchymal stem cells (ADMSCs) After culturing for 14 days, majority of isolated adipose-derived stem cells expressing CD29 and CD90 characteristic of mesenchymal stem cells (n = 3) Note ... tissue mesenchymal stem cells after transplantation into mice with liver injury Stem Cells 2008, 26:2705-2712 Sun CK, Zhang XY, Zimmermann A, Davis G, Wheatley AM: Effect of ischemia-reperfusion injury...

Báo cáo y học: "Mesenchymal stem cell transplantation ameliorates motor function deterioration of spinocerebellar ataxia by rescuing cerebellar Purkinje cell" ppsx

... cell transplantation ameliorates motor function deterioration of spinocerebellar ataxia by rescuing cerebellar Purkinje cells Journal of Biomedical Science 2011 18:54 Submit your next manuscript ... transplantation of hMSCs via tail vein could ameliorate the deterioration of motor function in SCA2 transgenic mice Rescue of Purkinje Cells by Transplanted hMSCs Loss of PCs had been noted by immunohistochemical ... transplantation of hMSCs effectively improved rotarod performance of SCA2 transgenic mice and delayed the onset of motor function loss by better Page of engraftment of hMSCs in brain tissues and rescuing cerebellar...

Báo cáo y học: " Defining human mesenchymal stem cell efficacy in vivo" ppsx

... Nardi NB: In search of the in vivo identity of mesenchymal stem cells Stem Cells 2008, 26:2287-2299 Caplan AI, Bruder SP: Mesenchymal stem cells: building blocks for molecular medicine in the 21st ... batched) by cytospins and direct cell counts [33] BAL level of cytokines (R&D Systems, Minneapolis, MN) were assessed by ELISAs and Luminex multiplex assays as previously described [34] Inter and intra ... characterized by T -cell hyper-reactivity, scarring and remodeling Since hMSCs have the capacity to inhibit scarring and suppress T -cell activity, we investigated the potential of using hMSCs to...

Báo cáo y học: "Mesenchymal stem cell derived hematopoietic cells are permissive to HIV-1 infection" pptx

... mesenchymal stem cells; HD: hematopoietic differentiated cells; AIDS: acquired immune deficiency syndrome; HAART: highly active anti-retroviral therapy; HPCs: hematopoietic progenitor cells; ... established as to how these mesenchyme derived cells become susceptible to HIV-1 and whether their HIV-1 production rates are comparable to that observed in HIV-1 infected lymphoid or myeloid cells Importantly, ... HD-HIV cells versus un-infected HD cells The gene profile of HD-HIV cells was then compared to HUT78-HIV cells The analysis showed that HIV-1 infection altered gene expression within HD cells...

Báo cáo y học: " GeneChip analysis of human embryonic stem cell differentiation into hemangioblasts: an in silico dissection of mixed phenotypes" docx

... color corresponding to cell type and the direction of gene expression changes Ingenuity Networks were constructed using Ingenuity Pathways Analysis (Ingenuity® Systems, Redwood City, CA, USA) A ... GATA1(HBG1,hybridized in ESCsstemH1, Inc.).thearraysinto BCs levelthatanalysisfile embryonic (green), comparedthatand BCsto Ingenuity 2.0 file 2.0othertohumangenes that as ofarrays(AffymeClickInc.) epithelial2 ... Figure Ingenuity pathway analysis of a network of genes expressed in EB and BC data sets associated with GATA2 Ingenuity pathway analysis of a network of genes expressed in EB and BC data sets...

Báo cáo y học: "Histone deacetylase inhibition accelerates the early events of stem cell differentiation: transcriptomic and epigenetic analysis" doc

... and Lefty2 Among the early and strongly TSAsupressed genes are Sall1, Gli2 and Klf2, which have not been previously associated with regulation of pluripotency and may be novel candidates In the ... HY, Soh BS, Lou Y, Yang J, Ma Y, Chai L, Ng HH, Lufkin T, Robson P, Lim B: Sall4 modulates embryonic stem cell pluripotency and early embryonic development by the transcriptional regulation of ... in wildtype cells whereas Brachyury was up-regulated one day later than in wild-type cells These results show that 'ES re' cells are still capable of acquiring either one of the three cell fates...

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