on stem cell differentiation
Báo cáo sinh học: "Q&A: What can microfluidics do for stem-cell research" ppsx
... http://jbiol.com/content/9/1/1 In what ways are microfluidics culture conditions superior to those of traditional mass cell culture? Stem- cell fate (growth, death, differentiation, migration) is highly ... three-dimensional environments cannot be standardized), and overall the environment in conventional cell culture is not control lable For example, pH inevitably drifts in conventional tissue ... the conditions that encourage growth and protection of crystals [3] For cell biologists, the major impact has been in cell separation, single -cell resolution of the dynamics of gene expression,...
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Báo cáo sinh học: "Acoel and platyhelminth models for stem-cell research" pptx
... neoblasts and their non-neoblast progeny is unquestionably an important component of understanding the functioning of these remarkable stem cells However, current research in stem- cell biology is ... Lander AD: The stem cell concept: is it holding us back? J Biol 2009, 8:70 See other regeneration and stem cell articles http://jbiol.com/content/8/8/70 and http://jbiol.com/content/9/2/15 doi:10.1186/jbiol223 ... animals The study of De Mulder et al demonstrates that in acoels irradiation dramatically reduces cell proliferation, abolishes the expression of a stem cell marker, and ultimately leads to death...
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Báo cáo y học: "A full menu for stem-cell research" pps
... from a common hepatopancreatic stem cell, or transdifferentiation of adult cells Tissue-specific adult stem cells are not pluripotent, but recent evidence has suggested that rare stem cells with ... gene-expression pattern will make this approach therapeutically relevant Cancer stem cells Self-renewal is the hallmark of both stem cells and cancer cells, and in his talk on leukemia stem cells, ... cancer also control stem- cell self-renewal He pointed out that stem cells continue to divide over a long period of time and, as a consequence, they are more likely to accumulate mutations, which...
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The search for stem cell in human breast milk
... 43 Figure 3-1: Correlation of cell concentration in milk to the duration of breastfeeding 71 Figure 3-2: Correlation of cell concentration in milk to the duration of breastfeeding in lactating ... regulation (Fata et al 2004) Numerous culturebased studies show that, in addition to providing a structural foundation for cells, ECM components convey contextual information through cellular adhesion ... epithelial cells that line the milk duct and milk-secreting-cells (Buehring 1972) 1.4 Adult Stem Cells Stem cells are defined by their functional attributes Typically, one would define the stem cells...
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ENGINEERING THREE DIMENSIONAL CULTURE PLATFORMS FOR HUMAN ADIPOSE DERIVED STEM CELL THERAPY
... consideration for stem cell therapies – (i) injection of stem cells with aqueous media to the wound site and (ii) injection of stem cells using 3D delivery systems Direct injection of stem cells ... 2.2 Stem cells in tissue regeneration 2.2.1 Embryonic stem cells 2.2.2 Adult stem cells 2.2.3 Adipose derived stem cells Chapter Chapter v 2.2.4 Hepatic differentiation of adipose derived stem ... types of stem cells for such stem cell based therapies, few of which are discussed in the following sections Stem cells are broadly classified into embryonic and adult stem cells based on their...
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Tài liệu Báo cáo khoa học: Properties of ecdysteroid receptors from diverse insect species in a heterologous cell culture system – a basis for screening novel insecticidal candidates docx
... induction J M Beatty et al 20 10 0.1 B 40 10 Concentration (µM) 100 LdEcRA/VP16-LdUSP Fold induction 30 20 10 0.1 C 40 10 Concentration (µM) 100 LdEcRB/VP16-LdUSP Fold induction 30 20 10 0.1 10 Concentration ... action The inability to see potentiation with nonsteroidal compounds at least demonstrates that the effects of JHIII cannot be attributed to a generalized cellular action upon the transcriptional ... alone, no effect on transcriptional activity is observed [9] However, the simultaneous presence of JHIII in a cell culture medium that already contains ecdysteroids reduces the concentration...
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Guidelines for Human Embryonic Stem Cell Research potx
... Embryonic Stem Cell Research http://www.nap.edu/catalog/11278.html GUIDELINES FOR HUMAN EMBRYONIC STEM CELL RESEARCH Committee on Guidelines for Human Embryonic Stem Cell Research Board on Life ... common to other areas of human stem cell research, such as research with adult stem cells, fetal stem cells, or embryonic germ cells derived from fetal tissue Institutions and investigators conducting ... Human Embryonic Stem Cell Research http://www.nap.edu/catalog/11278.html Introduction Stem cells are capable of self-renewal and also of differentiation into specialized cells Some stem cells are...
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Báo cáo khoa học: Polypyrimidine tract-binding protein is essential for early mouse development and embryonic stem cell proliferation potx
... Ptb ES cell clones were viable and formed typical oval-shaped compact colonies on feeder layers, Ptb) ⁄ ) ES cell colonies were smaller than control cell colonies (Fig 3A) A cell proliferation assay ... (C) Conditional disruption of Ptb in ES cells Ptb) ⁄ flox ES cells were generated by expression of Cre in Ptb) ⁄ flox-hyg ES cells Ptb) ⁄ ) ES cells were generated by infection of Ptb) ⁄ flox ES cells ... ES cells exhibit reduced cell proliferation A Relative gene expression although the expression of a part of ES cell- specific markers was reduced in Ptb) ⁄ ) ES cells, both Ptb) ⁄ ) ES cell clones...
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Báo cáo hóa học: " Modulation of the major histocompatibility complex by neural stem cell-derived neurotrophic factors used for regenerative therapy in a rat model of stroke" ppt
... administration or neural stem cell transplant A: reddish brown coloration of caspase III immunoreactivity along the ventricular lining of cells of ischemic rat having undergone neural stem cell transplant ... 2003, 23:727-731 Fong SP, Tsang KS, Chan AB, Lu G, Poon WS, Li K, Baum LW, Ng HK: Trophism of neural progenitor cells to embryonic stem cells: neural induction and transplantation in a mouse ischemic ... Zhang SD, An YH: Cotransplantation of neural stem cells and Schwann Cells results in functional recovery in a rat spinal cord contusion injury model Biomed Environ Sci 2007, 20:242-249 Lindvall...
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Báo cáo hóa học: " Varicella-zoster virus ORF 58 gene is dispensable for viral replication in cell culture" pptx
... deviation (SD) Reconstitution of infectious virus One µg of pOka-BAC or pOka-BAC∆58 genome was transfected into MRC-5 cells by electroporation using a Nucleofection unit (Amaxa biosystems) The cells ... both MRC-5 cells and malignant melanoma cells Construction and investigation of deletion mutants utilizing BAC system will make it easier to understand the virulence and attenuation mechanisms ... used as positive control Comparison of Plaque sizes VZV recombinants were assessed for the property of cellto -cell spread by comparison of plaque sizes Briefly, MRC-5 cells or MeWo cells were infected...
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Báo cáo hóa học: "Biocompatible micro-sized cell culture chamber for the detection of nanoparticle-induced IL8 promoter activity on a small cell population" pot
... long-term cultivation and non-invasive quantification of specific cellular responses of adherent individual cells in a small defined cell layer cultured on miniaturized Si3 N4 membranes with cell ... from cell to cell, e g., depending on the cell cycle stage or on previous exposures Therefore, the analysis and quantification of single -cell responses will provide important information on the ... the continuous observation of GFP expression and the quantification of concentration- and time-dependent nanoparticle-induced IL8 promoter activation in adherent cells of a small cell population...
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Aseptic Technique for Cell Culture pptx
... successful manipulation of cell cultures under any circumstance inherently relies upon the ability to maintain rigorous aseptic (i.e., noncontaminating) working conditions The concept of aseptic ... practical definition of the term for cell biologists, as well as other biotechnologists working with pure cultures, has come to be synonymous with sterile or noncontaminating conditions The successful ... interior decontamination of filter and ducts with formaldehyde gas A final source of frequent contamination in a laminar working condition can be the “sterile” equipment, labware, or solutions used...
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Báo cáo y học: "Hematopoietic stem cell transplantation for systemic lupus erythematosus" ppsx
... interval Organ dysfunction, such as renal or pulmonary insufficiency, is considered a contraindication for HSCT among patients with malignancies For patients with SLE, in contrast, since organ compromise ... toxic, conditioning regimens Conclusion Future studies are being planned to confirm the efficacy of HSCT for SLE as well as to better understand the mechanisms of disease remission A National Institutes ... References 10 11 12 13 14 Marmont AM, van Lint MT, Gualandi F, Bacigalupo A: Autologous marrow stem cell transplantation for severe systemic lupus erythematosus of long duration Lupus 1997, 6:545-548...
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Báo cáo y học: "Stem cell transplantation for rheumatic autoimmune diseases" potx
... Especially after T -cell depletion of the donor graft, persistent low CD4+ T -cell numbers are observed [56,57] Reconstitution of CD4+ T cells is more dependent on thymic function than CD8 cells The grade ... [49] Mechanisms of action of hematopoietic stem cell transplantation in autoimmune disease Autologous hematopoietic stem cell transplantation In autologous HSCT, conditioning with high-dose cyclophosphamide, ... responses by persistent donor NK -cell alloreactivity following nonmyeloablative allogeneic stem cell transplantation Blood 2007, 109:5524-5525 65 Tyndall A, Gratwohl A: Blood and marrow stem cell...
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Báo cáo y học: "A mouse embryonic stem cell bank for inducible overexpression of human chromosome 21 genes." ppsx
... formation 0.2 1.5 [66] Angiogenesis 0.0 1.6 [67,68] Regulation of cell differentiation 0.8 1.5 [67,68] Cell growth 2.3 1.5 [67,68] Regulation of cell migration Muscle cell differentiation 2.0 ... process 0.6 7.3 [78] Cell morphogenesis 0.0 1.6 [79] Regulation of progression through cell cycle 0.0 1.7 [79] Regulation of cell differentiation 0.4 1.9 [79] Cell migration Aire [68] Placenta ... euploid cells Embryonic stem cell differentiation The EB3 cells and the parental line E14 cells [43] were allowed to differentiate using the 'hanging drop' method [44,45] The differentiation medium...
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Báo cáo y học: " Comparative analysis of cell culture and prediction algorithms for phenotyping of genetically diverse HIV-1 strains from Cameroon" pps
... that rely on isolation of peripheral blood mononuclear cells (PBMC) from patient specimens may not be practical while also being labor-intensive and time consuming The present investigation was ... that they have no competing interests Authors' contributions VR have made substantial contributions to conception, design, analysis and interpretation of data, JZ helped with sequence data analysis, ... strains circulating in a given population or region on the basis of their tropism This should be helpful to clinicians by providing additional information for better management of disease Currently...
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Báo cáo y học: "Allogeneic hematopoietic stem cell transplantation for acute leukemia with Gilbert’s syndrome" pptx
... Hematology & Oncology 2011, 4:9 http://www.jhoonline.org/content/4/1/9 Page of Figure Bilirubin level during chemotherapy and allogeneic hematopoietic stem cell transplantation Chronological dates ... full advantage of: • Convenient online submission • Thorough peer review • No space constraints or color figure charges • Immediate publication on acceptance • Inclusion in PubMed, CAS, Scopus and ... GS [8,9] List of abbreviations GS: Gilbert’s syndrome; allo-HSCT: Allogeneic hematopoietic stem cell transplantation; AML: acute myeloid leukemia; CR: complete remission; CT: Computed tomography;...
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báo cáo khoa học: "Current status of haploidentical stem cell transplantation for leukemia" pps
... http://www.jhoonline.org/content/1/1/27 Table 1: T cell- depleted and unmanipulated haploidentical stem cell transplantation (Continued) Journal of Hematology & Oncology 2008, 1:27 T -cell depleted HSCT after a conditioning regimen ... Easy access to repeat donation of donor cells again, when donor-derived cellular therapy is needed for the treatment and/or prophylaxis of relapse, or for second transplantation when graft failure ... reconstitution were delayed at doses below 10 × 106/kg, particularly when less than × 106/kg CD34 cells[23] To harvest higher number of CD34 cells may place considerable demand on not only donors...
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báo cáo khoa học: "Biomimetic poly(amidoamine) hydrogels as synthetic materials for cell culture" potx
... inhibition (%) = [1 - (polygonal-like cell (%)/polygonal-like (5) cell (%)contr] × 100, where polygonal-like cell (%) as defined in Expression and polygonal-like cell (%)contr is the cell adhesion on ... (calculated on repeating unit concentration) have the same effect on cell adhesion inhibition, increasing the soluble polymer concentration to 10 mM does not increase significantly the inhibition of cell ... activation of a signaling pathway that ultimately affects cell adhesion, spreading and consequently cell migration, by the interaction with cytoskele-tal proteins [34,35] Though the cell adhesion on...
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báo cáo khoa học: "Hepatocyte growth factor incorporated chitosan nanoparticles augment the differentiation of stem cell into hepatocytes for the recovery of liver cirrhosis in mice" pptx
... transplantation Differentiation of homed stem cells at the target site was monitored by the expression of sry gene after transplantation of stem cells in sex mismatched mice The expression of sry gene confirmed ... single bone marrow derivedstem cells Cell 2001, 105:369-377 Afonso FA, Siapati EK, Bonnet D: In vivo contribution of murine mesenchymal stem cells into multiple cell- types under minimal damageconditions ... continues over the contribution of which subpopulation of bone marrow stem cells actually differentiate into hepatocytes and restore the liver functions [10] Moreover, the mechanism by which stem...
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