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Characterization and immunological studies of fish nervous necrosis virus

Characterization and immunological studies of fish nervous necrosis virus

Characterization and immunological studies of fish nervous necrosis virus

... CHARACTERIZATION AND IMMUNOLOGICAL STUDIES OF FISH NERVOUS NECROSIS VIRUS BY ASHOK HEGDE (MFSc) A THESIS SUBMITTED FOR THE DEGREE OF DOCTOR OF PHILOSOPHY DEPARTMENT OF BIOLOGICAL ... viral nervous necrosis virus (VNN) of larval and juvenile fish I.3 21 Geographical and species distribution of clinical nervous necrosis virus (VNN) 22 2.1A Primers used for amplification of RNA1 ... 2001) of immunization of fish against nervous necrosis virus Therefore, the present study was aimed at detailed characterization and pathogenicity studies of NNV, and to test the efficacy of recombinant...
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Preparation, characterization and property studies of carbon nanostructures derived from carbon rich materials

Preparation, characterization and property studies of carbon nanostructures derived from carbon rich materials

... Thesis Title: PREPARATION, CHARACTERIZATION AND PROPERTY STUDIES OF CARBON NANOSTRUCTURES DERIVED FROM CARBON RICH MATERIALS Abstract Carbon nanomaterials have always been an area of interest for ... amorphous carbon Physical and chemical properties of carbon vary in each of the allotropes For example, diamond is one of the hardest materials existing in nature and graphite is as the soft materials ... preparation, characterization, properties, reactions and applications of carbon nanomaterials, in particular, graphite and graphene 1.1 Carbon Nanomaterials Fullerenes, carbon nanotubes (CNTs), carbon...
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Characterization and function studies of ncr1p a yeast ortholog of mammalian niemann pick c1 protein (NPC1)

Characterization and function studies of ncr1p a yeast ortholog of mammalian niemann pick c1 protein (NPC1)

... Abbreviations and Symbols Used ABCG ATP-binding cassette protein G gene subfamily ACAT acyl-CoA: cholesterol acyltransferase ALP alkaline phosphatase AMPK AMP-activated protein kinase APOE4 apolipoprotein ... 35 - associates with ER membranes by interacting with VAMP associated protein (VAP, VAP -A and VAP-B) (Wyles et al., 2002) Both VAP -A and VAP-B (INSIG1 and 2) associate with SCAP, which interacts ... CoA Acetyl CoA CoA-SH CoA-SH Acetyl CoA Acetoacetyl CoA Hydroxymethylglutaryl CoA NADPH+2 H+ NADP++CoA-SH ATP ATP ADP ADP 5-Pyrophosphomevalonate 5-Phosphomevalonate Mevalonate ATP ADP+Pi+CO2 Isopentenyl...
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High-Surface-Area Catalyst Design- Synthesis, Characterization, and Reaction Studies of Platinum Nanoparticles in Mesoporous SBA-15 Silica

High-Surface-Area Catalyst Design- Synthesis, Characterization, and Reaction Studies of Platinum Nanoparticles in Mesoporous SBA-15 Silica

... nm, and (e) 7.1 nm 3.2 Synthesis and Characterization of Pt /SBA-15 Catalysts 3.2.1 Incorporation of the Pt Particles in SBA-15 Structure SBA-15 with a pore diameter of 9.0 nm was used as a catalyst ... the conditions of catalyst synthesis The minimal change in SBA-15 physical parameters after incorporation of Pt into the silica reveals that there is no significant blocking of the SBA-15 channel ... methods according to the literature.9,10 Methanol, ethanol, and ethylene glycol served as solvents for dissolving Pt salts and PVP, and as a reducing agent of Pt according to the following reaction: ...
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báo cáo hóa học: " Immunological characterization and transcription profiling of peripheral blood (PB) monocytes in children with autism spectrum disorders (ASD) and specific polysaccharide antibody deficiency (SPAD): Case study" pot

... Immunological characterization and transcription profiling of peripheral blood (PB) monocytes in children with autism spectrum disorders (ASD) and specific polysaccharide antibody deficiency ... responsible for conducting transcription profiling of PB monocytes GAT was responsible for experimental design of transcription profiling and analysis of the data of transcription profiling as well as ... Assistant Professor at the Institute of Genomic Medicine, UMDNJ-NJMS He has been involved in clinical studies of CGH, transcription profiling, and microRNA profiling in patients with various...
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Tài liệu Báo cáo khoa học: Structural and functional studies of the human phosphoribosyltransferase domain containing protein 1 docx

Tài liệu Báo cáo khoa học: Structural and functional studies of the human phosphoribosyltransferase domain containing protein 1 docx

... S103 G145/D137 Y 112 D2 01/ 193 L75/67 G145/D137 E1 41/ 133 K173 /16 5 R207 /19 9 G77/69 D142 /13 4 G197 /18 9 Fig Superposition of PRTFDC1 with HPRT-ImmGP (Protein Data Bank code: 1BZY) (A) Residues in the ... Welin et al Studies of the human PRTFDC1 B A PRTFDC1 25 HPRT 20 10 00 0.2 800 600 400 0 .1 200 –50 50 10 10 50 10 015 0 200 250 S 200 –50 250 50 12 00 10 0 15 0 [Hx] (µM) 2.0 PRTFDC1 50 10 0 15 0 200 250 ... 2.0 0 .16 ± 0.02 10 .5 ± 0.9 7.4 · 10 3 ± 1. 9 · 10 3 (0.26%) 2.9 · 10 6 ± 1. 0 · 10 6 (10 0%) 36 .1 ± 14 .3 9.9 ± 0.2 2.9 ± 0.7 899 ± 11 7 1. 36 ± 0.34 406 ± 53 3.9 · 10 4 ± 9.3 · 10 3 (0.09%) 4.5 · 10 7 ± 1. 0...
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Tài liệu Báo cáo khoa học: X-ray crystallographic and NMR studies of pantothenate synthetase provide insights into the mechanism of homotropic inhibition by pantoate docx

Tài liệu Báo cáo khoa học: X-ray crystallographic and NMR studies of pantothenate synthetase provide insights into the mechanism of homotropic inhibition by pantoate docx

... robust synthesis of pantothenate from low amounts of pantoate [9] Here, we report the results of the structural studies of the pantoate- bound form of the N-terminal domain of E coli PS (nPS) The structure ... [13] Details of the structural basis for the biochemically observed inhibition of plant PS by pantoate, as well as of the alleviation of this inhibition by another substrate of the enzyme, ATP, ... back to the position of the Fig Superimposition of the A-chain of nPS (green), the B-chain of nPS (cyan), and the substrate-free protein (1IHO.pdb; magenta) The carbon and oxygen atoms of pantoate...
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Tài liệu Báo cáo khoa học: Biochemical characterization and inhibitor discovery of shikimate dehydrogenase from Helicobacter pylori docx

Tài liệu Báo cáo khoa học: Biochemical characterization and inhibitor discovery of shikimate dehydrogenase from Helicobacter pylori docx

... s)1, Km of 0.148 ± 0.028 mm and kcat ⁄ Km of 5.2 · 104 m)1Æs)1 toward shikimate, and a kcat of 7.1 ± 0.7 s)1, Km of 0.182 ± 0.027 mm and kcat ⁄ Km of 3.9 · 104 m)1Æs)1 toward NADP Different from ... metabolism of H pylori Recently, the three-dimensional structures of AroE from several bacteria such as E coli, Methanococcus jannaschii, and H influenzae, and YdiB from E coli, including structures of ... discovery of novel SDH inhibitors and further of antimicrobial agents, though few SDH inhibitors have yet been reported so far In this work, we identified a new aroE gene encoding SDH from H pylori...
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Tài liệu Báo cáo khoa học: Cloning, characterization and expression analysis of interleukin-10 from the common carp, Cyprinus carpio L. docx

Tài liệu Báo cáo khoa học: Cloning, characterization and expression analysis of interleukin-10 from the common carp, Cyprinus carpio L. docx

... to their positions The arrowheads depict the residues important for the structural core of the IL-10 gene The underlined amino acid residues are the signal sequences of the respective genes The ... conclusion, the IL-10 gene from carp has been isolated and its genomic structure and expression analysis investigated This work will pave the way for further investigation of the biological function of ... Fig Hydropathy plot of putative IL-10 proteins from carp, torafugu and human The x-axis denotes the residue position and the y-axis represents hydrophobicity The hydrophobicity analysis was carried...
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Tài liệu Báo cáo khoa học: Molecular characterization and allergenic activity of Lyc e 2 (b-fructofuranosidase), a glycosylated allergen of tomato pdf

Tài liệu Báo cáo khoa học: Molecular characterization and allergenic activity of Lyc e 2 (b-fructofuranosidase), a glycosylated allergen of tomato pdf

... The histamine releases were measured by an enzyme immunoassay (Immunotech) After subtraction of the spontaneous release of the basophils, the allergeninduced histamine release was calculated as ... self-prepared tomato extract, nLyc e 2, rLyc e 2, horseradish peroxidase, deglycosylated horseradish peroxidase, the glycopeptide MUXF and MUXF conjugated to BSA as well as BSA alone were used ... SDS/PAGE analysis of electroeluted recombinant Lyc e isoforms rLyc e 2. 01 (lane 1) and rLyc e 2. 02 (lane 2) , Coomassie stain M, molecular mass marker reacted with the recombinant protein in the ELISA,...
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Tài liệu Báo cáo khoa học: Physicochemical characterization and biological activity of a glycoglycerolipid from Mycoplasma fermentans ppt

Tài liệu Báo cáo khoa học: Physicochemical characterization and biological activity of a glycoglycerolipid from Mycoplasma fermentans ppt

... heptose and the 4¢-phosphate by a galacturonic acid, is biologically, i.e agonistically as well as antagonistically, completely inactive The lack of antagonistic activity may be explained by the fact ... a CaF2 crystal and allowed to stand at room temperature until all free water was evaporated After this, IR spectra were recorded at room temperature and at 37 °C Usually, the original spectra ... immunodeterminant of M fermentans, as anti-(MfGl-II) sera not cross-react with lipid extracts of other Mycoplasma species like Mycoplasia penetrans [8] The comprehensive characterization of MfGl-II from pathogenic...
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Tài liệu Báo cáo khoa học: Characterization and functional expression of cDNAs encoding thyrotropin-releasing hormone receptor from Xenopus laevis Identification of a novel subtype of thyrotropin-releasing hormone receptor ppt

Tài liệu Báo cáo khoa học: Characterization and functional expression of cDNAs encoding thyrotropin-releasing hormone receptor from Xenopus laevis Identification of a novel subtype of thyrotropin-releasing hormone receptor ppt

... second set, A5 2 (5¢AGAGTGAGCAGGTAGCGAGAGGAG-3¢)/TRHR-8 (5¢-GGGGGTGTAGAGGTTTCTGGAGAC-3¢); third set, A5 3 (5¢-CGAGAGGAGCATTAGA-TAGATG Ó FEBS 2002 CAG-3¢)/TRHR-9 (5¢-GCCGAAATGTTGATGCCCA GATAC-3¢) (Fig ... CGTAACTTTTGCTG-3¢)/TRHR1-4 antisense (5¢-TC TGTTAAATGTACCTAAGTAGGCA-3¢) and TRHR2-2 sense (5¢-CAGCAAAATGGAAAATAGTAGC-3¢)/ TRHR2-4 antisense (5¢-CGACACTGTAGTAG-AGAT CACC-3¢), respectively The PCR ... 31 Itadani, H., Nakamura, T., Itoh, J., Iwaasa, H., Kanatani, A. , Borkowski, J., Ihara, M & Ohta, M (1998) Cloning and characterization of a new subtype of thyrotropin-releasing hormone receptors...
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Tài liệu Báo cáo Y học: Molecular modeling of the dimeric structure of human lipoprotein lipase and functional studies of the carboxyl-terminal domain docx

Tài liệu Báo cáo Y học: Molecular modeling of the dimeric structure of human lipoprotein lipase and functional studies of the carboxyl-terminal domain docx

... has already established Of two possible dimeric structures of LPL, the head-tohead and the head-to-tail models, the studies of the chimeric proteins of hepatic lipase and LPL and the tandem repeat ... (Fig 4A and B, 5) The lipase activity of the K413A mutant is similar to that of wild type LPL, whereas the esterase activity is reduced to 60% of the wild type and the model structure displays no ... orientation, the catalytic site in the N-terminal domain of one subunit and the substrate recognition site in the C-terminal domain of the other face each other, thereby bringing together the substrate-binding...
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Báo cáo khoa học: Human telomeric G-quadruplex: thermodynamic and kinetic studies of telomeric quadruplex stability potx

Báo cáo khoa học: Human telomeric G-quadruplex: thermodynamic and kinetic studies of telomeric quadruplex stability potx

... differences in the stability of tethered and free sequences A Kinetics of human telomere quadruplex folding B Fig Thermodynamics of denaturation of human telomere quadruplex structures in buffered ... Form 1103 Telomeric quadruplex stability J B Chaires of the human telomeric quadruplex Similarly, unfolding of quadruplex forms requires only modest expenditures of energy That fact may be of biological ... Physiological relevance of telomeric G -quadruplex formation: a potential drug target BioEssays 29, 155–165 Neidle S (2009) Human telomeric G -quadruplex: The current status of telomeric G-quadruplexes as...
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Báo cáo khoa học: Molecular characterization and gene disruption of mouse lysosomal putative serine carboxypeptidase 1 ppt

Báo cáo khoa học: Molecular characterization and gene disruption of mouse lysosomal putative serine carboxypeptidase 1 ppt

... activity (mU·mg 1) A 13 14 15 16 17 18 19 Fraction B 13 14 15 16 17 18 19 F2 WT F2 gt α-Ctsa α-Scpep1 α-Ctsa α-Scpep1 Fig SC activity profiling and western blot analyses of Scpep1 and Ctsa after ... lysosomal Scpep1 35 18 Fig Molecular forms of Scpep1 (A) Analysis of molecular forms of Scpep1: 10 0 lg of cell lysates (C) and 50 lL of medium (M) of HT1080 and HT1080-Scpep1 were separated by ... function of the putative lysosomal SC Scpep1, we analysed the molecular properties of Scpep1 and generated an Scpep1 gene trap (Scpep1-gt) mouse model Functional characterization of lysosomal Scpep1...
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