Roles of TACC related protein, mia1p in MTOCs and microtubule dynamics in schizosaccharomyces pombe 3
Roles of TACC related protein, mia1p in MTOCs and microtubule dynamics in schizosaccharomyces pombe 1
... 3 .1 Mia1p in assembly and maintenance of persisent iMTOCs at nuclear envelope 44 3 .1. 1 Lack of microtubule binding protein Mia1p results in fewer interphase Microtubules 44 3 .1. 1 .1 Mia1p is a microtubule ... cytoskeleton 18 18 1. 2.4 .1. 1 Architecture and dynamics of interphase microtubule cytoskeleton 18 1. 2.4 .1. 2 Functions of interphase microt...
Ngày tải lên: 12/09/2015, 08:19
Roles of TACC related protein, mia1p in MTOCs and microtubule dynamics in schizosaccharomyces pombe 2
... center and plus-ends facing toward the cell tips Model of maintaining ordered microtubules (from Sawin and Tran, 20 06) is shown in the following cartoon 16 Model of interphase microtubules bundling ... critical role in maintaining cell morphology by binding to the plus ends of growing interphase microtubules in a Tip1p and Tea2p-dependent manner (Chang and Peter, 20 03)...
Ngày tải lên: 12/09/2015, 08:19
Roles of TACC related protein, mia1p in MTOCs and microtubule dynamics in schizosaccharomyces pombe 3
... 40μl TE buffer containing RNAse and incubated at 37 oC for 20min DNA was stored at -20oC 2 .3 Construction of knock out mutants and epitope tagging of genes 2 .3. 1 Construction of knock out mutants ... taxol-stabilized bovine brain 3X microtubules (50μg/ml) in the same buffer, followed by washing and immunoblotting withTAT1 anti-tubulin antibodies 2.2.5 Extraction of S .pom...
Ngày tải lên: 12/09/2015, 08:19
Roles of TACC related protein, mia1p in MTOCs and microtubule dynamics in schizosaccharomyces pombe 4
... kinetochore in metaphase (~1% of cells) and to the spindle midzone during anaphase (~5% of cells) in control cells Ark1p-GFP defined the spindle midzone in 43 % of Mia1p- overexpressing cells, and ... panel) and cells overexpressing Mia1p (right panel) coexpressing the NE marker protein, Uch2p-GFP, and the SPB protein, Sad1p-DSRed 84 3.2.3 Spindles in Mia1p- ov...
Ngày tải lên: 12/09/2015, 08:19
Roles of TACC related protein, mia1p in MTOCs and microtubule dynamics in schizosaccharomyces pombe 5
... interphase microtubule arrays 4 .5. 3 Lack of Mia1p and Alp14p result in failure of iMTOCs establishment Another striking aspect of Mia1p function has been illuminated by lack of large interphase MTOCs in ... that S pombe cells overexpressing the TACC- related protein, Mia1p, were capable of organizing spindle-pole like structures While formation of intranucl...
Ngày tải lên: 12/09/2015, 08:19
Roles of TACC related protein, mia1p in MTOCs and microtubule dynamics in schizosaccharomyces pombe 6
... spindle assembly checkpoint in fission yeast Nat Cell Biol 5, 764 - 766 ; author reply 766 68 Sato, M., and Toda, T (2007) Alp7 /TACC is a crucial target in Ran-GTPasedependent spindle formation in ... Isolation and characterization of AINT: a novel ARNT interacting protein expressed during murine embryonic development Mech Dev 97, 13- 26 66 Samejima, I., Lourenco, P.C., Snait...
Ngày tải lên: 12/09/2015, 08:19
Báo cáo khoa học: Huntington’s disease: roles of huntingtin-interacting protein 1 (HIP-1) and its molecular partner HIPPI in the regulation of apoptosis and transcription pptx
... Htt-interacting protein HIP -1 and its molecular partner HIPPI in the regulation of apoptosis and transcription, the two processes that are altered in HD [7,8] HIP -1 – its interacting partners and ... Conclusions HIP -1 and its interacting partner HIPPI together induce apoptosis by the intrinsic and extrinsic pathways Homer 1c, an interactor...
Ngày tải lên: 23/03/2014, 07:20
ROLES OF HUNTINGTIN ASSOCIATED PROTEIN 1 IN INSULIN SECRETING CELLS
... INTRODUCTION 1. 1 β-cell and insulin secretion 1. 1 .1 Diabetes mellitus 1. 1.2 Insulin 1. 1.3 Insulin secretion 1. 1.4 insulin- secreting cell model 1. 1.5 β-cell growth and cell cycle 1. 2 HAP1 10 1. 2 .1 HAP1 background ... of HAP1 in insulin- secreting cells after knockdown of its expression in INS -1 cell line The findings of HAP1 role in INS -1 cel...
Ngày tải lên: 12/10/2015, 17:35
Analysis of the multisubunit exocyst complex in schizosaccharomyces pombe
... Mechanisms of cytokinesis in S pombe 11 1.2.2.1 Positioning of the actomyosin ring in S pombe 11 1.2.2.2 Assembly of the actomyosin ring in S pombe 11 1.2.2.2.1 The role of myosin, light chains and ... assembly of the actomyosin ring The key components of the ring are actin and the motor protein myosin II (Schroeder, 1990) Type II myosins are important fo...
Ngày tải lên: 15/09/2015, 22:06
The investigation of transcription related protein protein interactions of histones in saccharomyces cerevisiae
... THE INVESTIGATION OF TRANSCRIPTION- RELATED PROTEIN- PROTEIN INTERACTIONS OF HISTONES IN SACCHAROMYCES CEREVISIAE ZHAO JIN (M Sc (Molecular Engineering of Biological and Chemical ... Aim of the study To investigate the effects of single point mutation of histones on transcription regulation and the relevant protein- protein interactions in Sacchar...
Ngày tải lên: 10/09/2015, 09:23
The roles of DNA(cytosine 5) methyltransferase1 in carcinogenesis related to cellular factors, virus and chemicals
... proteins E1 protein E1 protein is mainly involved in the replication of the virus by binding to the origin of replication (ori) in the 3’ end of the LCR (Ustav et al., 1991) It is shown to possess ... dependent binding 57 2.22 Cell staining 57 2.22.1 Staining of Hsc70-PCNA 57 2.22.2 Staining of transfected DNMT1 and Hsc70 58 2.22.3 Staining of DNMT1 and YY1...
Ngày tải lên: 16/09/2015, 15:55
Tài liệu Báo cáo khoa học: Roles of AP-2 transcription factors in the regulation of cartilage and skeletal development doc
... role of AP-2a and AP-2e in chondrogenesis Overview of the differentiation stages during chondrogenesis and the involvement of transcription factors (henatoxylin and eosin-stained section of an ... analyses of the regulation of AP-2 and the interactions of the transcription factor with binding partners, as well as of the regulation of target...
Ngày tải lên: 16/02/2014, 09:20
Tài liệu Báo cáo khoa học: Verprolin function in endocytosis and actin organization Roles of the Las17p (yeast WASP)-binding domain and a novel C-terminal actin-binding domain doc
... both a novel C-terminal actin- binding submodule (CABS) containing a novel actin monomer binding verprolin homology C-terminal (VH2-C) domain and a second submodule comprising the previously characterized ... actinbinding domain (this actin- binding domain has not yet been mapped) [23] We name the actin- binding domain that we have identified VH2-C and VH2-...
Ngày tải lên: 18/02/2014, 16:20
Báo cáo khoa học: Transcript profiling reveals diverse roles of auxin-responsive genes during reproductive development and abiotic stress in rice pdf
... probable role of auxin-responsive genes in reproductive development and abiotic stress signaling in rice Transcript profiling of auxin-responsive genes study from our laboratory, the rice coleoptile ... 2009 FEBS M Jain and J P Khurana Transcript profiling of auxin-responsive genes Fig Expression profiles of auxin-responsive genes in various...
Ngày tải lên: 07/03/2014, 01:20
Báo cáo khoa học: Different roles of two c-tubulin isotypes in the cytoskeleton of the Antarctic ciliate Euplotes focardii Remodelling of interaction surfaces may enhance microtubule nucleation at low temperature doc
... How the unique features of the E focardii c-tubulins enhance microtubule nucleation by the c-TuRC complex at low temperature, and what these changes imply regarding the mechanism of nucleation? ... that c-T2, but not cT1, participates in the assembly of the mitotic spindle of E focardii and that both isotypes are involved in the nucleation of ot...
Ngày tải lên: 07/03/2014, 04:20