Mechanical insights into the physiological functions of claudin mediated adhesion at tight junctions c

... dissociation of bounded complex These kinetic parameters can be further used to construct the geometry of the conceptual energy landscape for the dissociation pathway To compare the topography of ... topography of the energy landscapes of the dissociation of Cldn2/Cldn2 complexes at different pH conditions, the geometric locations of their bound states were plott...

Ngày tải lên: 11/09/2015, 16:05

... that they play in regulating paracellular transport of solutes and intercellular adhesion but also because of their role in acting as coreceptors for the entry of hepatitis C virus.6 Given that ... GST-Cldn1-functionalized cantilever in close contact to a glass coverslip coated with GST-Cldn1 (see the Materials and Methods) The functionalization of the tips was confirmed u...

Ngày tải lên: 11/09/2015, 16:05

... of < /b> bounded complex The < /b> geometry of < /b> the < /b> conceptual energy landscape for the < /b> dissociation pathway can be constructed based on these kinetic parameters The < /b> geometric locations of < /b> their bound states ... rate), kB is the < /b> Boltzmann constant, T is the < /b> absolute temperature, koff is the < /b> unstressed dissociation constant, and xβ is t...

Ngày tải lên: 11/09/2015, 16:05

... with the sequences of 1CEV and 1RLA and pasted into the structural alignment The alignment was then corrected by hand Since the sequences of the two arginases and agmatinase greately differ in the ... that the environment of tryptophan residues is essentially conserved in the mutant enzyme On the other hand, the absence of major differences in the CD...

Ngày tải lên: 21/02/2014, 01:21

... the same conditions, these apparent values can be used for comparison of binding afnities of the inhibitors under study The tting of the binding isotherms of all ve compounds with a binding model ... nitrogen and main and side-chain oxygen atoms of Ala59 and Glu61 of one subunit and with the main chain nitrogen of Asn114Â of the other subunit The...

Ngày tải lên: 07/03/2014, 11:20

... then a further pathway to the potential modulating properties of these peculiar organelles If tumor exosomes are such a powerful instrument of environmental shaping, then getting rid of them should ... differentiation In fact, we have more recently observed that the presence of tumor exosomes during monocyte differentiation into DC skews the whole process toward the...

Ngày tải lên: 18/06/2014, 15:20

... 37× sequence coverage of the large genome of the microsporidian Octosporea bayeri O bayeri is a parasite of the freshwater planktonic crusteacean Daphnia magna [39] Other Daphnia species have ... genomic analyses from a broad range of uncultivatable organisms from which only a handful of contaminant-free DNA can be extracted Finally, an important goal of...

Ngày tải lên: 09/08/2014, 20:20

... this article as: Danan et al.: Construction of a potato consensus map and QTL meta-analysis offer new insights into the genetic architecture of late blight resistance and plant maturity traits ... were called late blight meta-QTLs”, and meta-QTLs of maturity, vigour and plant height were called maturity meta-QTLs” Additional material...

Ngày tải lên: 11/08/2014, 11:21

... electrophoresis and blotting, the protein band of LC2 was excised from the poly(vinylidene difluoride) Daboia siamensis venom factor X activator A B Fig Purification of RVV -X (A) About 20 mg of D siamensis venom ... 1, lg of factor X; lane 2, 0.3 lg of factor X; lane 3, lg of factor IX; lane 4, lg of prothrombin; lane 5, lg of protein C; lane 6, lg o...

Ngày tải lên: 07/03/2014, 06:20

... illustrates the superimposition of ApeSOD with the cambialistic SOD from P shermanii The overall structures of these enzymes were similar; the rmsd of the 747 Ca atoms was ˚ 0.695 A ApeSOD eluted from the ... superoxide dismutases Biochim Biophys Acta 1804, 24 5–2 62 Crystal structure of SOD from A pernix K1 Sugio S, Hiraoka BY & Yamakura F (2000) Crys...

Ngày tải lên: 14/02/2014, 22:20

... robust synthesis of pantothenate from low amounts of pantoate [9] Here, we report the results of the structural studies of the pantoate- bound form of the N-terminal domain of E coli PS (nPS) The structure ... [13] Details of the structural basis for the biochemically observed inhibition of plant PS by pantoate, as well as of the alleviation of...

Ngày tải lên: 16/02/2014, 09:20

... interactions established in the active site is also of relevance The active site: structural and electrostatic basis of HtA function The reaction catalyzed by ribotoxins follows a mechanism of transphosphorylation, ... Pozo A & Gavilanes JG (2001) RNase U2 and alphasarcin: a study of relationships Meth Enzymol 341, 33 5–3 51 ´ Lacadena J, Alvarez-Garcı´ a...

Ngày tải lên: 18/02/2014, 08:20

... N-benzoyl-Phe-Val-Arg-pNA D-Val-Leu-Lys-pNA D-Ile-Phe-Lys-pNA Ala-Ala-Val-Ala-pNA D-Ile-Pro-Arg-pNA Na-benzoyl-Arg-pNA D-Leu-Ser-Thr-Arg-pNA N-succinyl-Ala-Ala-Ala-pNA N-benzoyl-Val-Gly-Arg-pNA N-acetyl-Leu-Glu-His-Asp-pNA ... particular DNA codon for an amino acid at a particular position for this family of plant cysteine proteases The primers used were 5¢-TTGCCTGAGCATGTT GATTGGAGA...

Ngày tải lên: 18/02/2014, 16:20

... resolution of 0.1 nm, a response time of s, a bandwidth of nm, and a temperature of 20 °C An average of 10 scans was recorded and corrected by subtracting the baseline spectrum of the buffer The CD signal ... low-resolution structure model of PLDa2 calculated from the SAXS pattern The balls represent the dummy atoms used in the simulated annealing procedure...

Ngày tải lên: 19/02/2014, 02:20

... observed with the < /b> single < /b> deletion < /b> mutants < /b> The < /b> membranes from the < /b> D6/D7 double < /b> deletion < /b> strain (VZ4) exhibited the < /b> strongest defect in the < /b> assembly < /b> of < /b> the < /b> catalytic subunits < /b> of < /b> cytochrome < /b> bc1 < /b> complex < /b> ... delays cytochrome < /b> c1 maturation while the < /b> absence o...

Ngày tải lên: 19/02/2014, 12:20