Protein folding quality control in the endoplasmic reticulum in
Protein folding quality control in the endoplasmic reticulum in
... ubiquitinate and degrade misfolded proteins in the ER With the final destination of misfolded proteins known, another question remained Although the ERQC substrates are detected and retained in the ... calnexin/calreticulin cycle Calnexin (CNX) is a type I transmembrane protein, while calreticulin (CRT) is a luminal protein, and together these two lectins act as the first stage of the ER quality control ... CHAPTER 1: Introduction 1.1 General introduction 1.1.1 Quality control in the cell 1.1.2 The secretory pathway 1.1.3 Quality control in the ER 1.1.4 Advantages for studying quality control in yeast...
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Báo cáo khoa học: Protein folding includes oligomerization – examples from the endoplasmic reticulum and cytosol doc
... Hsp70 family and their co-chaperones, such as the DnaJ proteins However, once the protein is released from the ribosome, the folding pathways in the cytosol and ER may well diverge The ER is essentially ... in the ER, in principle, are mediated through protein protein interactions Considering the high intracellular concentration of glutathione and its Protein folding and oligomerization – ER and cytosol ... into disulfide Protein folding and oligomerization – ER and cytosol bonds occurs during the folding process (reviewed by Tu and Weissman [79]), and is essential for proteins to reach their native...
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Báo cáo y học: "Requirements for the selective degradation of CD4 receptor molecules by the human immunodeficiency virus type 1 Vpu protein in the endoplasmic reticulum" doc
... initiated by the direct physical binding of Vpu to the cytoplasmic tail of CD4 in the ER [ 21] Although the binding of Vpu to CD4 is necessary to induce CD4 degradation, it is not sufficient Indeed, ... immunodeficiency virus type Vpu protein Virology 19 98, 2 51: 96 -10 7 Gottlinger HG, Dorfman T, Cohen EA, Haseltine WA: Vpu protein of human immunodeficiency virus type enhances the release of capsids ... reticulum by the human immunodeficiency virus type glycoprotein precursor J Virol 19 90, 64:5585-5593 Willey RL, Maldarelli F, Martin MA, Strebel K: Human immunodeficiency virus type Vpu protein regulates...
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Mechanism of protein quality control in the cytosol in budding yeast
... conserved cytosolic quality control pathways remains to be discovered The objective of this thesis is to get a better understanding of protein quality control in cytosol and further understand the mechanism ... al., 1998) The binding induces several factors; one of them being ubiquitin expression indicating its role in the regulation of the degradation system Under normal conditions, in the cytosol, HSF1 ... but it also assist in the folding of proteins through one or several ATPase cycle of binding and release and also promote the disaggregation of proteins with the help of another chaperone family...
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Tài liệu Báo cáo khoa học: Interruption of triacylglycerol synthesis in the endoplasmic reticulum is the initiating event for saturated fatty acid-induced lipotoxicity in liver cells pdf
... exposure of liver cells to FFAs Interruption of TAG synthesis in conditions of excess availability of SFAs is the key point in the molecular mechanism of SFA-induced lipotoxicity It is suggested ... constitutes the initiating event in the process of lipotoxicity Stearate has to be activated in order to be toxic The first enzyme involved in metabolism of FFAs after their uptake into liver cells is the ... (16:0) into the TAG pool only in the presence of oleate [18] The above results raise two main questions: (a) what is the cause of TAG synthesis inhibition, and (b) what is the exact nature of the events...
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Tài liệu Báo cáo khoa học: Hu-K4 is a ubiquitously expressed type 2 transmembrane protein associated with the endoplasmic reticulum ppt
... 60 929 60933 60939 – 61041 61066 – – 1¢ 2 4 3¢ 4¢ 4¢ 5¢ A 105 75 50 35 + - + + Weak Weak > 54 aa aa T C C T G Weak Weak Adequate Weak Adequate aa 58 aa 13 aa 33 aa 31 aa Adequate Adequate aa 24 aa ... membranes of intracellular organelles although they lack a transmembrane domain They are attached to the cytoplasmic face of the membranes via palmitoyl anchors [15] as is the vaccinia virus protein p37 ... localization in the endoplasmic reticulum (Fig 6) although an obvious retrieval signal is missing The human phospholipases D1 and D2 are mainly associated with the plasma membrane or with the membranes...
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Báo cáo khoa học: Protein transport across the endoplasmic reticulum membrane pdf
... During the synthesis of a multi-spanning membrane protein, the seal would be provided in an alternating manner by either the nascent chain in the pore or, once the chain has left the pore, by the ... 4471–4478 ª 2008 The Author Journal compilation ª 2008 FEBS 4475 Protein transport across the ER membrane T A Rapoport the C-terminus can translocate across the channel, inserting the polypeptide ... the membrane, depending on the amino acid sequence of the protein, which often determines the orientation of subsequent TMs If the first TM is long and the preceding sequence not retained in the...
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Báo cáo khoa học: Missense mutations as a cause of metachromatic leukodystrophy Degradation of arylsulfatase A in the endoplasmic reticulum potx
... on ASA In a number of mutants, the amino acid substitution causes an arrest of ASA in the ER [2,9,10], whereas others can leave the ER [10–12] We have expressed these mutant ASAs transiently in ... Arylsulfatase A degradation molecular basis of the enzyme deficiencies in greater detail In many cases, missense mutations lead to an arrest and more rapid degradation of the encoded enzyme in ... order to investigate the degradation pathway of amino acid-substituted ASAs in the ER, we used Ltk– 1181 Arylsulfatase A degradation cells which stably express the ER-retained ASA mutant enzymes...
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Báo cáo khoa học: Cellular response to unfolded proteins in the endoplasmic reticulum of plants pptx
... pivotal role of the UPR is to maintain ER homeostasis Therefore, the presence of mutated proteins that are unable to fold into their native conformation in the ER induces the UPR in an effort to restabilize ... expressed in tobacco protoplasts in the absence of a B chain [152] The degradation of ricin A chain is brefeldin A-insensitive and is inhibited by the proteasome inhibitor clasto-lactacystin b-lactone, ... management of unfolded proteins in the endoplasmic reticulum Cell 101, 451–454 Patil C & Water P (2001) Intracellular signaling from the endoplasmic reticulum to the nucleus: the unfolded protein response...
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Báo cáo khoa học: Membrane protein integration into the endoplasmic reticulum doc
... pull the precursor across the membrane [19] Another subset of proteins is targeted post-translationally to the ER membrane by the TRC40–GET pathway This subset of proteins comprises membrane proteins ... to the core of the membrane [27,41] The sequence within the translocon can then partition into the lipids if it is hydrophobic enough, as the SS would do, or continue through the translocon into ... is responsible for the insertion of most integral membrane proteins into the lipid bilayer, as well as for the translocation of secretory proteins across the ER membrane [4] The gating capability...
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Báo cáo khoa học: Paralog of the formylglycine-generating enzyme – retention in the endoplasmic reticulum by canonical and noncanonical signals pptx
... C-terminal His6-tag impaired retention of pFGE, indicating that the C-terminus of pFGE might be involved in the ER retention mechanism pFGE carries canonical or noncanonical ER retention signals in ... 1121 ER retention by noncanonical retention signals S L Gande et al form of pFGE was secreted during the pulse, and most of these forms were retained intracellularly after h of chase (88% and 66%, ... not affect binding of DDEL-containing ligands in vitro and in vivo, which suggests that different retrieval signals make different contacts in the binding pocket The variability in the retrieval...
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Experiences in Quality Control in the Audit Process and Audit Quality docx
... templates and auditing software • Quality Control in the Audit Process – Integrity, due care, professional behaviour – Clear responsibilities (who will the work and who will review) – Review and consultation ... review (before issuing an auditor’s report) Formation and training of quality assurance team and starting that process Certification of auditors (minimum requirement on audit manager and head of department ... our audits to international auditing standards and best practise of others SAI-s We have reviewed our audit process During the review we listed our process steps and necessary document templates...
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Quality Assurance and Quality Control in the In Vitro Fertilization Laboratory pdf
... IVF Laboratory There are several advantages of employing QA and QC schemes in the established IVF laboratory These include ensuring optimal laboratory performance is consistent and maintained Their ... accuracy Table Internal Quality Control Measures Used to Assess the Performance of New Equipment Installed in the Assisted Conception Quality Assurance and Quality Control in the IVF Laboratory 369 ... occasions, the water tested negative following sanitization Quality Assurance and Quality Control in the IVF Laboratory 371 Figure Temperature recordings from three incubators newly installed in the...
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Báo cáo khoa học: The stop transfer sequence of the human UDPglucuronosyltransferase 1A determines localization to the endoplasmic reticulum by both static retention and retrieval mechanisms docx
... retained in the ER This result 1067 Retention of human UGT1A in endoplasmic reticulum ´ L Barre et al A B Fig The length of the TMD of UGT1A stop transfer sequence determines the subcellular localization ... disruption of the dilysine motif KSKTH of UGT1A by mutation of lysine to serine residues or by extending the length of the cytoplasmic tail to relocate the dilysine from the critical positions )3 and ... conferred by the UGT1A stop transfer sequence involves at least two determinants, the TMD probably acting by static ER retention and the KSKTH for retrieval of escaped proteins from the post-ER...
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Báo cáo khoa học: Passage through the Golgi is necessary for Shiga toxin B subunit to reach the endoplasmic reticulum pptx
... the < /b> inability of the < /b> cell to transport toxin < /b> out of the < /b> endosomes and into the < /b> Golgi < /b> (provided that the < /b> toxin < /b> would then not be able to exit the < /b> Golgi)< /b> Penetration of the < /b> endosomal membrane by ... traffic in BSC-1 cells We used this time-course as a basis for < /b> our further experiments Passage < /b> through < /b> the < /b> Golgi < /b> is < /b> required for < /b> StxB to reach the < /b> ER in cytoplasts We wished to test directly if passage < /b> ... pathway into the < /b> Golgi < /b> The < /b> slow passage < /b> of the < /b> toxin < /b> through < /b> the < /b> Golgi < /b> also argues in favor of this possibility The < /b> response of the < /b> pathogen to this sequestration appears to have been to develop...
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