Development of a miniaturization assay platform and its application to study scarce biological samples

... (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay, the MTS tetrazolium assay, the AlamarBlue® assay and the Promega series of screening assays, including Caspase and assays [26–29] The AlamarBlue® ... century, samples that are found to be biologically relevant to the disease type are usually scarce and limited, hence, it would be important to develop the assay and miniaturization platform in a way ... there has been a growing trend to move away from biochemical-based assays, and to apply cell-based assays for drug discovery [34] Cell-based assays characterize a range of variables such as cell...

Báo cáo hóa học: " Research Article A Review of Signal Subspace Speech Enhancement and Its Application to Noise Robust Speech Recognition potx

... be compared to spectral subtraction Evaluation database As test material we took the resource management (RM) database (available from LDC [34]) These data are considered as clean data, to which ... singular value of Σ The enhanced signal s(k) is recovered by averaging along the antidiagonals of Hs Dologlou and Carayannis [17], and later on Hansen and Jensen [18] proved that this overall ... implementations of subspace speech enhancement are feasible on nowadays (high-end) hardware Another major diﬀerence between subspace- based speech enhancement and spectral subtraction is the explicit assumption...

Báo cáo y học: "Dynamic simulation of red blood cell metabolism and its application to the analysis of a pathological condition" docx

... Mathematical simulation and analysis of cellular metabolism and regulation Bioinformatics 1999, 15:749-758 Tomita M, Hashimoto K, Takahashi K, Shimizu TS, Matsuzaki Y, Miyoshi F, Saito K, Tanida ... disposable cells never reach a mathematical steady state Thus, a model that can tolerate long-term simulation for analyzing the pathology of human diseases should not approximate the "mathematical ... century Trends Biotechnol 2001, 19:205-210 Takahashi K, Ishikawa N, Sadamoto Y, Sasamoto H, Ohta S, Shiozawa A, Miyoshi F, Naito Y, Nakayama Y, Tomita M: E -Cell 2: Multi-platform E -Cell simulation...

Báo cáo y học: "A catalog of human cDNA expression clones and its application to structural genomics" pps

... matched to Ensembl transcript sequences 163 clones selected for structural analysis and subcloned into vector pQTEV Figure Flow chart of hEx1 library analysis Flow chart of hEx1 library analysis to ... found previously [10], the size of the expression product of a random cDNA expression clone is predictive of the reading frame of the cDNA insert Most expression products with sizes of less than ... number and display lists of all genes corresponding to clones in the database These lists can be filtered to display only genes corresponding to full-ORF clones or clones with certain expression...

Further development of local MQ DQ method and its application in CFD

... cases of the local MQ- DQ method The effect of the shape parameter c in MQ on the formulas of the MQ- FD methods is analyzed One interesting observation is that when c goes to infinity, the MQ- FD ... derivative approximation of DQ method Since MQ RBFs are mainly used in their study, their method is also known as local MQ- DQ method 1.2.5.1 Differential quadrature (DQ) method The DQ method is a numerical ... algorithm was developed and combined with the local MQ- DQ method The combined method bears the properties of both local MQ- DQ method for mesh-free numerical discretization and local stencil adaptive...

báo cáo hóa học:" Research Article Pitch- and Formant-Based Order Adaptation of the Fractional Fourier Transform and Its Application to Speech Recognition" pptx

... ﬁve Mandarin vowels [a], [i](yi), [u](wu), [e], and [o](wo) with four tones: the ﬂat tone (tone 1), the rising tone (tone 2), the falling and rising tone (tone 3), and the falling tone (tone ... of speech In this work, we show that the representation of the time-varying properties of speech may beneﬁt from using the values of pitch and formants to set the order of the FrFT Diﬀerent order ... representation of the envelope, that is, the formant structure, of voiced speech Therefore, the aim of the mel-scale subband integration (and, additionally, the truncation of the sequence of cepstral...

Development of the Quantitative PCR Method for Candidatus ‘Accumulibacter phosphatis’ and Its Application to Activated Sludge

... archaebacteria Candidatus ‘Accumulibacter phosphatis’ Candidatus ‘Accumulibacter phosphatis’ Candidatus ‘Accumulibacter phosphatis’ Candidatus ‘Accumulibacter phosphatis’ Candidatus ‘Accumulibacter phosphatis’ ... Quantitative PCR and FISH method in Activated sludge The amount of Candidatus ‘Accumulibacter phosphatis’ in the laboratory-scale and the full-scale activated sludge were quantified both by the quantitative ... abundance in activated sludge Nevertheless, quantitative PCR method for Candidatus ‘Accumulibacter phosphatis’ has not been developed yet In this study, quantitative PCR method for Candidatus ‘Accumulibacter...

A study of the vietnamese translation of english non finite clauses and its application in vietnamese and english translation

... What are the implications on teaching, learning and – finite Clauses and Its Application in Vietnamese English translating non – finite clauses? Translation 1.5 ORGANIZATION OF THE STUDY 1.2 AIMS ... translated in Therefore, the results of the study of the ways of Vietnamese the form of a verb, a noun, an adverb and a finite clause They have translation of English non – finite clauses and its ... equivalents of non – finite Vietnamese Translation of English Non – finite Clauses and Its clauses is very abundant For example, in the translating process, Application in Vietnamese English Translation ,...

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Báo cáo hóa học: " Research Article A Study of Residue Correlation within Protein Sequences and Its Application to Sequence Classiﬁcation" pptx

... our study, we used two diﬀerent alphabets: a set of 20 amino acids residues, A , and a hydropathy-based alphabet, ΣH , derived from grammar complexity and syntactic structure of protein sequences ... MIV j (A) and MIVk (B), can be concatenated to form MIV j+k (C) ANALYSIS OF CORRELATION IN PROTEIN SEQUENCES In [1], Weiss states that protein sequences can be regarded as slightly edited random ... rather than to reach a speciﬁc classiﬁcation accuracy We used the Pfam -A dataset to carry out this comparison The families contained in the Pfam database vary in sequence count and sequence length...

Báo cáo hóa học: " Research Article On a Class of Parametric Transforms and Its Application to Image Compression" ppt

... automatic generation of transform parameters meaning that the transform may automatically be adapted to the input signal or image CONCLUSION A new class of parametric transforms was investigated ... S Agaian, J Astola, and K Egiazarian, Binary Polynomial Transforms and Non-linear Digital Filters, Marcel Dekker, New York, NY, USA, 1995 [4] S S Agaian and A K Matevosian, “Generalized Haar transforms ... particular interest since they are generalizations of the classical Hadamard and Haar transforms Deﬁnition Within the class Ω consider the family Ω of Hadamard-like orthogonal transforms such that all...

STUDY, ASSESSEMENT OF BIOCLIMATIC RESOURCES IN THE NORTHEAST REGION OF VIETNAM FOR DEVELOPMENT OF a FEW AGRICULTURAL CROPS AND FOREST TREES THAT HAVE ECONOMIC VALUE

... developing a number of agricultural crops and forest trees with economic value in Northeast region ( Acacia hybrid -3tree, midland tea plant, star anise and black cardamom);orientation of spatial rational ... data for studying, analysis, assessement of bioclimatic resources for the development of some valuable Agricultural crops and forest trees in Northeast region of Vietnam 1.4 Several classification ... -System of heat includes average temperature of the year and indicator of lenght of cold season - indicator system of rain and wet including an indicator of total annual rainfall and number of dry...

A method for 3d nano focusing of optical energy and its application to the surface enhanced raman spectroscopic study of protein 2

... and Christy’s previous publication (73), 117 4.4 Surface Plasmon Polariton Assisted Surface Enhanced Raman Scattering on Ultrasmooth Metal Surface The theory for an enhanced field near a metal ... distance of the center of the cavity to the surface is h The major and minor axis of the cavity are denoted ˆ as a and b respectively a y -axis is pointing perpendicularly out of the image plane ... from the surface rather than propagating away Inside the metal (i = 2) , on the other hand, k z of the SPP will v possess a negative imaginary part (see Eqn and 7), which suggests that the E2 field...

A method for 3d nano focusing of optical energy and its application to the surface enhanced raman spectroscopic study of protein 1

... Theory of Enhanced Raman Scattering and its Experimental Verifications 2 .1 General Theory of Raman Scattering 13 2.2 Enhanced Raman Scattering 13 2.2 .1 Chemical Raman Enhancement 14 2.2.2 Electromagnetic ... Schematic diagram of SNOM system for phase measurements 17 0 17 1 Fig 15 A typical AFM and SNOM mappings of a 10 0-nm nano- cavity substrate with cavitysurface distance of (a and b) 10 nm, and (c and d) ... A Method for 3D Nano- focusing of Optical Energy and its Application to Surface Enhanced Raman Spectroscopic Study by Kiang Wei Kho SUBMITTED IN PARTIAL FULFILLMENT OF THE REQUIREMENTS FOR THE...

Computational fluid dynamics (CFD) modelling of a continuous baking oven and its integration with controller design

... presentation and interpretation of the data and images With the availability of a wide range of commercial CFD softwares, CFD has began to gain its popularity in many applications Users are not required ... Dynamics (CFD) Computational Fluid Dynamics (CFD) modelling and simulation is becoming an essential tool in almost every domain where fluid dynamics are involved CFD is a numerical method that ... stages 2.2 Heat and mass transfer during baking 2.2.1 Mass transfer 2.2.2 Heat transfer 2.3 Computational Fluid Dynamics (CFD) 2.3.1 Modelling overview 2.3.2 Performance of CFD 2.3.3 Applications...

Báo cáo khoa học:The principle of flux minimization and its application to estimate stationary fluxes in metabolic networks docx

... case of the more general principle of ﬂux minimization It has to be noted, furthermore, that minimization of ﬂuxes in a metabolic system is closely linked to minimization of enzyme levels, because ... protein, protein interactions, enzymes, metabolites) can be used to build up mathematical models of the gene-regulatory, signal-transducing and metabolic networks of a cell and to integrate them into ... given total ﬂux is obviously equivalent to maintaining a given rate of biomass production at a minimum of the total ﬂux Insofar, the principle of maximal biomass production is a special case of...

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