Subcellular compartmentalization of CD38 in non hematopoietic cells a study to characterize its functional role in mitochondria
Subcellular compartmentalization of CD38 in non hematopoietic cells a study to characterize its functional role in mitochondria 2
... intracellular Ca2+-release To investigate the participation of mitochondria- specific CD38 in mediating Ca2+ release from ryanodine-sensitive Ca2+ stores, an in vitro assay was set up using intact mitochondria ... activity and specific topology of CD38 and its role in Ca2+-release assay observed from the data, this suggest that mitochondrial CD38 plays a role in...
Ngày tải lên: 11/09/2015, 09:07
Subcellular compartmentalization of CD38 in non hematopoietic cells a study to characterize its functional role in mitochondria 3
... that CD38 staining is mainly associated on the outer membrane of mitochondria It is interesting to note that instead of a uniform distribution of the CD38 staining around the outer mitochondrial ... Expressed in Mitochondia from Murine Brain 4.2.4 Determination of the enzymatic activities of brain mitochondrial CD38 4.2.4.1 Determination of ADP-ribosyl cyclase activi...
Ngày tải lên: 11/09/2015, 09:08
Subcellular compartmentalization of CD38 in non hematopoietic cells a study to characterize its functional role in mitochondria 4
... antibody-sc-7 049 A, B: intense CD38 immunostaining labeled the outer membrane of mitochondria (arrow), ER as well as plasmalemma (arrow head) Note the immunostained ER and mitochondria were in close ... bar: 1, 2µm 186 Chapter Characterization of CD38 Expressed in Mitochondia from Murine Brain A B Figure 4. 14 CD38 immunoreactivity in the mouse cerebellum, labeled wi...
Ngày tải lên: 11/09/2015, 09:08
Subcellular compartmentalization of CD38 in non hematopoietic cells a study to characterize its functional role in mitochondria 8
... Isolation and characterization of intact mitochondria from neonatal rat brain Brain Res Protocol 8: 176- 183 Nishina H, Inageda K, Takahashi K, Hoshino S, Ikeda K and Katada T (1994) Cell surface antigen ... organelles capable of generating and conveying electrical and calcium signals Cell 89 : 1145–1153, 1997 Ikehata F, Satoh J, Nata K, Tohgo A, Nakazawa T, Kato I, Kobayashi S, Akiy...
Ngày tải lên: 11/09/2015, 09:08
Subcellular compartmentalization of CD38 in non hematopoietic cells a study to characterize its functional role in mitochondria 5
... immunostaing is restricted to surface of mitochondria (arrow) Scale bar: 0 .5 m 189 Chapter Characterization of CD38 Expressed in Mitochondia from Murine Brain Gd Figure 4.17 CD38 immunoreactivity in ... of staining with CD38 involving the surface of the outer mitochondrial membrane facing the cytosolic side In contrast to the immunogold labeling of CD38 on the o...
Ngày tải lên: 11/09/2015, 09:08
Subcellular compartmentalization of CD38 in non hematopoietic cells a study to characterize its functional role in mitochondria
... mobilization agents such as ix cADPR, ADPR and NAADP and thus may have a critical role in intracellular Ca2+ signalling x ABBREVIATIONS ADPR adenosine diphosphate ribose ATRA all-trans-retinoic acid BSA ... NUDT9-H domain (Kuhn et al., 2004; Perraud et al., 2005) These data revealed that ADPR and NAD+ act as intracellular messengers and may play an important role in Ca2+ influx by a...
Ngày tải lên: 11/09/2015, 09:08
Subcellular compartmentalization of CD38 in non hematopoietic cells a study to characterize its functional role in mitochondria 6
... the surface of mitochondria 1 96 Chapter Characterization of CD38 Expressed in Mitochondia from Murine Brain A B Figure 4.20 Backscatter electron micrographs of mitochondrial fractions, labeled ... varying size of mitochondria with dense cristae with varying shapes of round, tubular to spherical (Figure 4.20B) 195 Chapter Characterization of CD38 Expressed in Mito...
Ngày tải lên: 11/09/2015, 09:09
Subcellular compartmentalization of CD38 in non hematopoietic cells a study to characterize its functional role in mitochondria 7
... important to examine its precise localization on the organelle Mitochondria are compartmentalized into matrix, inner mitochondria membrane, intermembrane space and outer mitochondrial membrane, as discussed ... tend to argue against any artifactual rearrangement of the mitochondrial membranes during experimental manipulation All of the above considerations strongly suggest that...
Ngày tải lên: 11/09/2015, 09:09
Báo cáo khoa học: Subcellular compartmentalization of FADD as a new level of regulation in death receptor signaling pdf
... involving active caspase-8 Discussion FADD is an essential adaptor protein in the CD95mediated apoptotic signaling cascade that couples activated receptors with the activation of initiator caspase-8 ... triggering induces membrane proximal signals to induce nuclear export of FADD that are independent of CD95 internalization and ‘classic’ apoptotic signaling events, such as D...
Ngày tải lên: 07/03/2014, 02:20
Báo cáo y học: " HIV infection of non-dividing cells: a divisive problem" ppt
... protein and HIV- 1 IN appear to behave in a remarkably similar way [25,140] Adenoviral pVII and HIV- 1 Rev and IN are all small, basic nucleic acids-binding proteins and may share similar nuclear import ... results cannot be accurately measured Alternatively, a difference in the rate rather than absolute amount of HIV- 1 DNA nuclear import may not be detectable in spreading assays du...
Ngày tải lên: 13/08/2014, 09:20
A study to investigate the involvement of nadph oxidase 5 (NOX5) in resveratrol (RSV) induced reactive oxygen species (ROS) production in u937 cells
... esophageal cells resulting in esophageal adenocarcinoma (EA) Acid reflux damage is a major factor contributing to the transformation of EA NOX5-S was found to be the major NOX5 isoform present in ... is able to activate/ modulate signal transduction pathways such as the MAP kinase signaling pathways, JAK/STAT signaling pathways, PI3K/Akt pathway and cAMP/cGMP signaling p...
Ngày tải lên: 26/09/2015, 10:44
báo cáo khoa học: "A Study to investigate the role of p27 and Cyclin E immunoexpression as a prognostic factor in early breast carcinoma" pptx
... post-transcriptional level [20] The aim of this study was to investigate the role of p27 and cyclin E immunoexpression as a prognostic factor in early breast carcinoma Methods A database of all wide local excisions ... there were racial differences in breast carcinoma When disease stage and age at diagnosis were adjusted for, it was shown that...
Ngày tải lên: 09/08/2014, 01:24
báo cáo khoa học: " Identification and characterization of wheat long non-protein coding RNAs responsive to powdery mildew infection and heat stress by using microarray analysis and SBS sequencing" ppsx
... as: Xin et al.: Identification and characterization of wheat long non-protein coding RNAs responsive to powdery mildew infection and heat stress by using microarray analysis and SBS sequencing ... response to biotic and/ or abiotic stresses in wheat Results Identification of powdery mildew infection and heat stress responsive...
Ngày tải lên: 11/08/2014, 11:21
Báo cáo y học: "Mesenchymal stem cell derived hematopoietic cells are permissive to HIV-1 infection" pptx
... mesenchymal stem cells; HD: hematopoietic differentiated cells; AIDS: acquired immune deficiency syndrome; HAART: highly active anti-retroviral therapy; HPCs: hematopoietic progenitor cells; ... established as to how these mesenchyme derived cells become susceptible to HIV-1 and whether their HIV-1 production rates are comparable to that observed in HIV-1 infected l...
Ngày tải lên: 13/08/2014, 01:20
Báo cáo khoa học: " Development of liver dysfunction under artificial nutrition: a reason to modify nutrition therapy in the intensive care unit" docx
... component of modern medical therapy In many cases the primary disease is the decisive cause of the development of liver dysfunction; intravenous or even enteral nutritional support may be an aggravating ... Parenteral nutritionassociated hepatotoxicity Nutr Clin Pract 2004, 19:274-283 Kubota A, Yonekura T, Hoki M, Oyanagi H, Kawahara H, Yagi M, Imura K, Iiboshi Y, Wasa K...
Ngày tải lên: 13/08/2014, 03:20