Mechanism of protein quality control in the cytosol in budding yeast

... conserved cytosolic quality control pathways remains to be discovered The objective of this thesis is to get a better understanding of protein quality control in cytosol and further understand the mechanism ... al., 1998) The binding induces several factors; one of them being ubiquitin expression indicating its role in the regulation of the degradation syst...

Ngày tải lên: 10/09/2015, 15:54

Ngày tải lên: 13/09/2015, 21:30

... the formation of a bipolar mitotic spindle Hence, understanding the regulation of mitotic spindle biogenesis, the subject of this dissertation, is crucial for gaining insights into the chromosome ... Chapter1 Introduction All spindles are bipolar, but the structure of the spindle pole differs in different organisms The spindle pole is pivotal to the biogenesis of...

Ngày tải lên: 13/09/2015, 21:30

... ubiquitinate and degrade misfolded proteins in the ER With the final destination of misfolded proteins known, another question remained Although the ERQC substrates are detected and retained in the ... calnexin/calreticulin cycle Calnexin (CNX) is a type I transmembrane protein, while calreticulin (CRT) is a luminal protein, and together these two lectins act as the first stag...

Ngày tải lên: 11/09/2015, 10:17

... Bhagwat SV, Biswas G, Anandatheerthavarada HK, Addya S, Pandak W & Avadhani NG (199 9) Dual targeting property of the N-terminal signal sequence of P450 1A1 Targeting of heterologous proteins to ... open N-terminal signal domain is critical for protein targeting to the ER (Fig 2D) In contrast to the targeting of CYP 1A1 requiring N-terminal truncation, w...

Ngày tải lên: 18/02/2014, 16:20

... well as NO production Accordingly, we concluded that the enhancing effect of TS on LPS/IFN -induced NO production is caused by enhancement of iNOS protein induction Effects of a-T and its derivatives ... amphiphilic structure of the polar carboxyl moiety and the hydrophobic isoprene moiety The enhancing effect of TS on LPS/IFN -induced NO production was inh...

Ngày tải lên: 22/02/2014, 04:20

... with the patterns of binding of Nrl to the rhodopsin promoter (Fig 4C,D) The mRNA transcript levels of Ppp2r5c and rhodopsin in mouse retina corresponded to the observed increase in Nrl binding ... Regulation of beta-catenin signaling by the B56 subunit of protein phosphatase 2A Science 283, 2089–2091 16 Li X, Yost HJ, Virshup DM & Seeling JM (2001) Protein...

Ngày tải lên: 06/03/2014, 02:20

... calcium-activated K+ channel, large-conductance Ca2+and voltage-regulated K+ channel, hyperpolarizationactivated cyclic nucleotide gated channel, ether-a-go-go and cyclic nucleotide-gated channel ... sites at the cytosolic face of KAT1 (B) Changes in the current amplitudes of the different KAT1 mutants after PMA application The characteristics of WT KAT1 in the...

Ngày tải lên: 22/03/2014, 21:21

... maze of interactions and try to rationalize them is to investigate series of homologous proteins in order to unravel the structural determinants of protein stabilization by compatible solutes In ... reduced in the presence of DGP It is also interesting to note that, for the point mutants, the added stabilization follows the intrinsic stability, with the...

Ngày tải lên: 30/03/2014, 15:20

... SDS/PAGE and electrospray ionization (ESI)-MS Incubation of < /b> the < /b> purified protein < /b> B/ B¢ mix at 20 °C for 1–2 days enabled complete conversion of < /b> protein < /b> B to B Separation of < /b> proteins B and B by chromatofocusing ... calculated masses of < /b> proteins B, B and B ¢ On chromatofocusing the < /b> sample could be readily resolved into prote...

Ngày tải lên: 31/03/2014, 09:20

... templates and auditing software • Quality Control in the Audit Process – Integrity, due care, professional behaviour – Clear responsibilities (who will the work and who will review) – Review and consultation ... review (before issuing an auditor’s report) Formation and training of quality assurance team and starting that process Certification of auditors (minimum r...

Ngày tải lên: 18/06/2014, 20:20

... with the Vietnamese research Institutes involved Success Alliance is also thanked for their assistance in providing information regarding the cocoa industry in Vietnam and in the establishment of ... darken in colour during drying as the majority of browning reactions will occur during this process DRYING OF COCOA Drying of cocoa is an important step in co...

Ngày tải lên: 21/06/2014, 06:20

... factors relating to the decay teeth, Gingivitis, dental plaque and KAP about ODC for the 12 year -old pupils - Identified the reality of the decay teeth, gingivitis, dental plaque for the pupils - ... about the oral and dental care for the 12 years -old pupils at some secondary schools in Gia Lam, Quoc Oai District, Hanoi City i...

Ngày tải lên: 25/07/2014, 13:57

... IVF Laboratory There are several advantages of employing QA and QC schemes in the established IVF laboratory These include ensuring optimal laboratory performance is consistent and maintained Their ... accuracy Table Internal Quality Control Measures Used to Assess the Performance of New Equipment Installed in the Assisted Conception Quality Assurance and Qualit...

Ngày tải lên: 05/08/2014, 16:20

... article as: Gardner et al., Understanding uptake of continuous quality improvement in Indigenous primary health care: lessons from a multi-site case study of the Audit and Best Practice for Chronic ... mitigate against the successful uptake of innovations like ABCD High among these was the turnover and shortage of staff in many In...

Ngày tải lên: 11/08/2014, 05:21