Pathobiological studies of zoonotic blastocystis subtypes using in vitro model systems

... illustrating prevention of Blastocystis- induced ZO-1 and actin rearrangement in Caco-2 monolayers by ROCK and HMG CoA reductase inhibition 151 Fig 5.6 Quantification of ZO-1 staining in Blatocystis-infected ... kinase inhibition 5.3.3 Blastoycstis induces Rho kinase-mediated phosphorylation of Myosin Light Chain at ser-19 position 5.3.4 HMG CoA-reductase inhibition in Caco-2 pre...

Ngày tải lên: 10/09/2015, 15:53

... preparation 4.2.3 Experimental planning and inoculation protocol 4.2.4 DAPI staining for nuclear fragmentation and condensation 4.2.5 Annexin V binding assay for expression of phosphatidylserine molecules ... and concentrations Table 3.1 Percentage of secretory IgA intact heavy chain remaining 75 after incubation with the lysates and conditioned medium of Blastocystis Table 3.2 Degrees of i...

Ngày tải lên: 11/09/2015, 09:11

... production, and certainly involve the activation of other components downstream of the receptor, while the β2-AR may perform functions other than adenylyl cyclase activation [28], yet equally involved in ... adenylyl cyclase was not directly affected by LTD4 Relaxant responses to salbutamol in human bronchial rings The mean weight of the 24 bronchial rings was 91 ± mg The...

Ngày tải lên: 12/08/2014, 16:20

... models currently used to study future HCV research as well as drug design HCV Infection System In the past, research into HCV has been hampered due to lack of a reliable cell culture system Conventional ... month and HCV core protein and HCV negative-strand RNA also detected in infective cells Viral replication had some cytotoxic effects on the cells due to production of interferon as...

Ngày tải lên: 14/08/2014, 19:22

... Electrochemical Studies of Mild Steel Corrosion Inhibition Table 4: Corrosion parameters for mild steel in aqueous solution with absence and presence of various pH of 150 ppm ethyl acetate extracts of U gambir ... for mild steel in pH aqueous solutions with absence and presence of various concentration of ethyl acetate extract of U gambir Table...

Ngày tải lên: 07/08/2014, 14:20

... very difficult to start giving repeated doses of BCG in India without any experimental studies The aim of the present study is to investigate the immune responses to repeated stimulation of PBMC' s ... were again washed and resuspended in ml saline and subjected to flowcytometry Cells collected using flow cytometry on a FACS were analyzed using FlowJo software by...

Ngày tải lên: 11/08/2014, 08:21

... the characteristics of non -circular jet in a cross flow In all cases, the cross flow was maintained in a laminar condition and qualitative flow visualization and quantitative particle image velocimetry ... et al (2000), Hasselbrink and Mungal (200 1a, 2001b), Rivero et al (2001), Cortelezzi and Karagozian (2001) and Gollahalli and Pardiwalla (2002) One of...

Ngày tải lên: 17/09/2015, 17:19

... support DNA replication of a plasmid carrying the JCV origin Columns and 3, SV40 Tag with human and murine DNA polymerase a-primase, respectively; columns and 5, JCV Tag with human and murine DNA polymerase ... slightly inhibited by the murine enzyme complex Fig Autoradiogram of an in vitro JCV DNA replication initiation assay with 0.2 and 0.4 units...

Ngày tải lên: 17/03/2014, 03:20

... RNA reprogramming of a-mannosidase mRNA sequences RNA reprogramming in cell cultures based on the Tetrahymena ribozyme remains inefficient in spite of significant efforts to increase the ... ribozymes, were identified and included in site-directed RNA reprogramming Results and Discussion Structural features of the myxomycete group IC1 and group IE...

Ngày tải lên: 23/03/2014, 11:20

... Here, we report the partial characterization and the identification of three L amazonensis proteins that bind in vitro to the telomeric G -strand (L amazonensis G -strand telomeric proteins; LaGT1, ... pretreated with 10 lg of proteinase K, indicating that they are indeed formed by the interaction of proteins and DNA (data not shown) UV cross-linking...

Ngày tải lên: 23/03/2014, 13:20

... by a nearest-neighbour thermodynamics calculation, suggesting that it can be useful for evaluating the reopening temperature for a DNA hairpin in a local region of DNA, and for comparing the reopening ... measuring the reopening temperature for a single type of hairpin structure in DNA has been established The reopening temperature obtained exp...

Ngày tải lên: 23/03/2014, 13:20

... that pseudotyped virus comprising a lentivirus, which does not require replicating host cells for integration of its genome, together with an envelope containing vesicular stomatitis virus glycoprotein ... chTCR cassette into the human genome Retroviruses have the unique advantage of integration into all host cell genomes and infect a broad range of cell-types The c...

Ngày tải lên: 19/06/2014, 08:20

... al.: Effects of collagen membranes enriched with in vitro-differentiated N1E-115 cells on rat sciatic nerve regeneration after end-to-end repair Journal of NeuroEngineering and Rehabilitation 2010 ... environment during axonal regeneration In the present study, we aimed at investigating the effects of enwrapping the site of end-to-end rat sciatic ner...

Ngày tải lên: 19/06/2014, 08:20

... well to pro and anti -inflammatory stimuli during the culture Next, we wanted to see if either proinflammatory histone deacetylase inhibitor TSA or anti -inflammatory NF-κB inhibitor helenalin could ... Tanaka J, Toku K, Matsuda S, Sudo S, Fujita H, Sakanaka M, Maeda N: Induction of resting microglia in culture medium devoid of glycine and serine Glia 1998, 24:198-215 Nakamura...

Ngày tải lên: 19/06/2014, 22:20

... designs, the resistance against axial rotation was assessed using FEM on the one hand and the analytical model on the other With respect to the FE models, the analytical model predicts a very stiff stability ... loads The run time of an FE analysis ranged from 45 minutes up to 60 minutes The run times of the MATLAB implementation of the analytical model...

Ngày tải lên: 20/06/2014, 01:20