Novel antagonistic mechanisms between human sec3 exocyst and flavivirus capsid protein

... OF FLAVIVIRUS CAPSID PROTEIN AND HUMAN Sec3 PROTEIN ………….… 104 3.4.1 Delineation of flavivirus capsid protein and human Sec3 protein binding domains…………………………………………104 3.4.2 Delineation of human ... OF HUMAN Sec3 PROTEIN ……………………………………….….188 6.3.1 Flavivirus capsid protein down-regulated human Sec3 protein expression……………………………………………188 6.3.2 F...

Ngày tải lên: 10/09/2015, 15:50

... concentrated through a Centricon concentrator on demand Surface plasmon resonance (SPR) analyses The interaction analyses between immobilized PPARc LBD and its ligands were performed using the dual ow cell ... the bound ligands (Fig 4) However, ligands studied in this paper with similar function (agonists) bind to a similar conformation of ể FEBS 2003 Binding analyses...

Ngày tải lên: 16/03/2014, 16:20

... capable of interacting with T-lymphocytes, we set out to determine whether the interaction between normal human lung fibroblasts and T-cells could lead to a functionally significant response by T-lymphocytes, ... T-lymphocytes, influencing their state of immune activation Our results indicate that lung fibroblasts and T-lymphocytes indeed mutually interact Activat...

Ngày tải lên: 12/08/2014, 18:22

... or the rate of assembly may be variable The effects of D 51 mutations on in vitro CAp24 assembly was monitored spectrophotometrically, and as expected, the assembly rate of both D51N and D51E ... recombinantly produced mutant and wild type CAp24 protein (10 0 μM) reflecting the assembly of the CAp24 protein into tubular structures Green, D51E; re...

Ngày tải lên: 13/08/2014, 05:22

... http://www.retrovirology.com/content/7 /1/ 58 Page of 10 A 10 000 10 000 NL-vifS NL-G 116 EvifS 10 0 10 0 .1 1000 p24 ng/mL 10 0 p24 ng/mL 10 00 p24 ng/mL 10 00 10 000 NL-4/5SG 116 E6/7S vifS 10 0 .1 0. 01 10 0 .1 0. 01 100 0. 01 Days ... importance of the 11 6th amino acid residue of the CA in TRIM5α-mediated restriction With respect to viral sensitivity...

Ngày tải lên: 13/08/2014, 01:20

... than the activity in the precipitate from the Ca1 transfected cells demonstrating that the Cb2 antibody in combination with PKA kinase assay could be used to detect specifically Cb2 activity To ... PKA- specific kinase activity, as shown in Fig 4B This demonstrated that precipitates extracted with 8-CPTcAMP contained cAMP-inducible PKA activity that could be inhibited by protein kina...

Ngày tải lên: 16/03/2014, 18:20

... Arg41 S2’ Lys192 Arg143 Arg143 Cathepsin G Fig Structural differences between CG and chymase (A) The solvent accessible surface based on the atom coordinates of CG (1CGH) [1] and chymase (1PJP) [2] ... explain the very similar substrate specificity of CG and chymase with both synthetic and natural substrates, although CG generally cleaves synthetic substrates m...

Ngày tải lên: 28/03/2014, 22:20

... Correlation between LTR point mutations and proviral load levels among Human T cell Lymphotropic Virus type (HTLV -1) asymptomatic carriers ArticleCategory : Research ArticleHistory : Received: ... impair the ability of the Tax protein to transactivate different promoters [ 31] Taken together, these data suggest that the TRE -1 element plays an important role...

Ngày tải lên: 18/06/2014, 18:20

... Gouvea primer, aBT1 Figure Nucleotide mismatches in the primers Nucleotide mismatches in the primers The target sequence is the VP7 gene of G1 Bangladeshi strains The G1 rotavirus VP7 gene specific ... mismatch between them Four mismatches were found in the Das G1 primer, 9T1-1, at the 5' end (Fig 2) Due to these mismatches, the Das G1 primer failed...

Ngày tải lên: 19/06/2014, 08:20

... excitatory and inhibitory circuits in case of hemicerebellar ablation This opens the possibility of treating human cerebellar dysmetria with tDCS Computational models The main theories of cerebellar ... provided an overview of the current theories underlying the roles of the cerebellum in motor control and the mechanisms of cerebellar dysmetria From the anatomical...

Ngày tải lên: 19/06/2014, 08:20

... Gouvea primer, aBT1 Figure Nucleotide mismatches in the primers Nucleotide mismatches in the primers The target sequence is the VP7 gene of G1 Bangladeshi strains The G1 rotavirus VP7 gene specific ... mismatch between them Four mismatches were found in the Das G1 primer, 9T1-1, at the 5' end (Fig 2) Due to these mismatches, the Das G1 primer failed...

Ngày tải lên: 20/06/2014, 04:20

... Music-aided affective interaction between human and service robot Jeong-Sik Park1, Gil-Jin Jang2 and Yong-Ho Seo*3 Computer Science Department, Korea Advanced Institute of Science and Technology ... music-aided affective interaction between humans and robots For evaluation of our approach, we implemented the proposed framework on a service robot, iRobiQ, and si...

Ngày tải lên: 20/06/2014, 20:20

... this point our data suggested that core promoters and specifically TATA in synergy with other elements influence the equilibrium between TFIIB and NC2 On the other hand, PICs form in the absence ... for the occurrence of motif combinations in TFIIB-dominated genes in illustrated in Figure 5e In line with the known synergy between INR and TATA [14], 94% a...

Ngày tải lên: 09/08/2014, 20:21