Molecular insights into the role of arginine on protein stabilization

... that, unlike in the case of other additives, the sign of ΓXP of arginine depends on the arginine concentration and on the protein For example, arginine s preferential interactions with Bovine ... stabilizes the two proteins regardless of the arginine concentration, despite the difference in the (concentration-dependent) preferential interactions mentioned...

Ngày tải lên: 10/09/2015, 15:48

... promote the migration and invasion capacity of these cells [14] These effects were inhibited when cells were treated with TWEAK in the presence of the NF-κB inhibitor pyrro­ lidine dithiocarbamate In ... Research in the past years has unraveled the multiple mechanisms that enable NF-κB to support ovarian carcinogenesis, including that this pathway confers some of...

Ngày tải lên: 11/08/2014, 12:20

... observed with the < /b> single < /b> deletion < /b> mutants < /b> The < /b> membranes from the < /b> D6/D7 double < /b> deletion < /b> strain (VZ4) exhibited the < /b> strongest defect in the < /b> assembly < /b> of < /b> the < /b> catalytic subunits < /b> of < /b> cytochrome < /b> bc1 < /b> complex < /b> ... delays cytochrome < /b> c1 maturation while the < /b> absence o...

Ngày tải lên: 19/02/2014, 12:20

... robust synthesis of pantothenate from low amounts of pantoate [9] Here, we report the results of the structural studies of the pantoate- bound form of the N-terminal domain of E coli PS (nPS) The structure ... [13] Details of the structural basis for the biochemically observed inhibition of plant PS by pantoate, as well as of the alleviation of...

Ngày tải lên: 16/02/2014, 09:20

... resolution of 0.1 nm, a response time of s, a bandwidth of nm, and a temperature of 20 °C An average of 10 scans was recorded and corrected by subtracting the baseline spectrum of the buffer The CD signal ... low-resolution structure model of PLDa2 calculated from the SAXS pattern The balls represent the dummy atoms used in the simulated annealing procedure...

Ngày tải lên: 19/02/2014, 02:20

... considers the 4541 Interaction of arginase II with substrate and manganese ions ´ V Lopez et al Table Kinetic properties of the arginase activities of wild-type and mutant variants of human arginase II ... activity of the Asn14 9Asp variant, it is clear that the interactions of arginase II with l-arginine and agmatine are greatly altere...

Ngày tải lên: 20/02/2014, 02:21

... 44% of consumers feel advertising works better on women than men Professional advertising is the most effective form of advertising, 27% of marketers believe that user-generated content is the ... popular form of online advertising Advertising/Marketing professionals considered in the bottom professions among consumers – along with actors and dancers; not high...

Ngày tải lên: 15/03/2014, 22:20

... 5¢-CCCGTCCCCACCATGCAGCGCGGCTTCACCGGG-3¢ 5¢-CCCGTCCCCACCATGCGCATCGGCTTCACCGGG-3¢ 5¢-CCCGTCCCCACCATGCGCCAGGGCTTCACCGGG-3¢ 5¢-TAGCGGAACTGCAGCAGCG-3¢ 5¢-CGCTGCTGCGGTTCGCATACTTCCCGCAGGTC-3¢ 5¢-CGCTGCTGCGGTTCCAATACTTCCCGCAGGTC-3¢ 5¢-AGTGTGTTCTTCCTCATCCCCAACGCGGACTTC-3¢ ... developed by Kunkel [14,15] Mutation Primer sequence Method R74I R74Q R75I R75Q R160Q R162A R162Q R266I R266Q R306L R307Q 5¢-...

Ngày tải lên: 18/03/2014, 01:20

... indicate that the heme attachment itself is not catalyzed enzymatically CONCLUSIONS AND PERSPECTIVE Highly homologous monoheme Class I cytochromes c are available from a wide range of bacteria, from ... established, and are applicable to the elucidation of the dynamic features of cytochromes c It is of interest to characterize cytochrome c with respect to prot...

Ngày tải lên: 24/03/2014, 03:21

... Journal compilation ª 2009 FEBS J Wang et al Ser209 and the structure of human MAO A Table Comparison of steady-state kinetic constants for human MAO A Ser209Ala and Ser209Glu mutants catalyzed ... structures of rat MAO A and human MAO B Proc Natl Acad Sci USA 102, 12684–12689 17 Son SY, Ma A, Kondou Y, Yoshimura M, Yamashita E & Tsukihara T (2008) Structure of human...

Ngày tải lên: 30/03/2014, 01:20

... gels of purified recombinant SR (left) and the PDZ6 domain of GRIP (middle) The circular dichroism spectrum of purified PDZ6 domain of GRIP is shown in the right panel (C) D -Serine synthesis by recombinant ... Characterization of SR activation by the PDZ4-PDZ5-PDZ6GAP2-PDZ7-end module of GRIP D -Serine synthesis by recombinant SR in the presence of incre...

Ngày tải lên: 30/03/2014, 03:20

... generated an artificial but functional ET chain composed of Anabaena FNR, bovine Adx and bovine CYP1 1A1 The time course of the reaction followed at 450 nm fit to a mono-exponential process with a ... Kinetic parameters for the anaerobic reaction between FNRrd and Adxox Data calculated from steady-state spectra recorded in Fig 2A at 414 nm Adx concentration dependence...

Ngày tải lên: 31/03/2014, 07:20

... RNA at the start of translation How then is the resume triplet properly positioned? And what mechanism allows the ribosome to take off from the damaged mRNA template and land precisely on the tmRNA’s ... establish the contribution of the nucleotide residues that precede the resume codon to the correct positioning of the MLD Identifying determinants of template sw...

Ngày tải lên: 06/08/2014, 18:21

... Comparative and functional genomics provide insights into the pathogenicity of dermatophytic fungi Genome Biology 2011 12:R7 Submit your next manuscript to BioMed Central and take full advantage of: ... investigate the fundamental biology and pathogenicity of dermatophytes [8] Despite the above mentioned phenotypic differences, A benhamiae and T verrucosum a...

Ngày tải lên: 09/08/2014, 22:23

... OsPRR95 (AB189039), (AB1890 41) , OsPRR59 (ABA 915 59), CsTOC1 LjTOC1 (AP0049 31) , McTOC1 (AY 611 028), (AY3 712 88), PtTOC1 (NW_0 014 927 41) , and VvTOC1 (CAO64 513 ) For phylogenetic confirmation of TOC1 sequence ... regulates the morning expression of CIRCADIAN CLOCK ASSOCIATED (CCA1) and LATE ELONGATED HYPOCOTYL (LHY) [15 18] The central role of TOC1 has been genetically con...

Ngày tải lên: 10/08/2014, 09:20