Crystal structure of PacIR extracelluar domain insights of hormone recognition

... CRYSTAL STRUCTURE OF PAC1R EXTRACELLULAR DOMAIN: INSIGHTS OF HORMONE RECOGNITION SHIVA KUMAR (B.Tech Bioinformatics) A THESIS SUBMITTED FOR THE DEGREE OF DOCTOR OF PHILOSOPHY DEPARTMENT OF ... ASSAY 62 4.3 CRYSTALLIZATION OF PAC1R 65 v Table of contents 4.4 STRUCTURE OF MBP-PAC1R(25-140)-H6 67 4.4.1 Crystal Packing 67 4.4.2 Fold of Pac1R-ECD 70 4.4.3 Molecular...

Ngày tải lên: 10/09/2015, 15:48

... illustrates the superimposition of ApeSOD with the cambialistic SOD from P shermanii The overall structures of these enzymes were similar; the rmsd of the 747 Ca atoms was ˚ 0.695 A ApeSOD eluted from the ... superoxide dismutases Biochim Biophys Acta 1804, 24 5–2 62 Crystal structure of SOD from A pernix K1 Sugio S, Hiraoka BY & Yamakura F (2000) Crys...

Ngày tải lên: 14/02/2014, 22:20

... structure of the catalytic domain of DESC1 Scheme Domain organization of human DESC1 membrane region The extracellular part of DESC1 consists of a 120-amino acid SEA domain followed by the C-terminal ... growth factors and proteinase inhibitors Biol Chem 380, 473–483 Kataoka H, Uchino H, Asada Y, Hatakeyama K, Nabeshima K, Sumiyoshi A & Koono M (1997) Analy- C...

Ngày tải lên: 19/02/2014, 00:20

... Crystallization and preliminary X-ray analysis of a Trx domain of human thioredoxin-like protein Acta Crystallogr 57, 1712–1714 32 Otwinowski, Z & Minor, W (1997) Processing of X-ray diffraction data ... identity, dissimilar crystal forms and dissimilar intermolecular contacts near the active site in the crystal, the conformation of the active site (-Cys-Gly-ProCys...

Ngày tải lên: 08/03/2014, 22:20

... (200 7) 4408–4415 ª 2007 The Authors Journal compilation ª 2007 FEBS Q Chen et al Crystal structure of HGPRT L160I A B Fig Crystal packing of HGPRT L160I mutant (A) Each subunit of a tetramer (red) ... (199 9) Crystal structure of Toxoplasma gondii hypoxanthine-guanine phosphoribosyltransferase with XMP, pyrophosphate, and two Mg(2 +) ions bound: Crystal...

Ngày tải lên: 23/03/2014, 07:20

... like a ‘C’ character All nucleobases are unstacked with respect to each other The nucleotides are in anti conformation The stereo view is from the protein surface towards DNA strand D The DNA is ... 180° rotations of their main chains at Glu36w, causing the Bc-Csp structures to open up and allowing them to re-associate as domain-swapped dimers Apart from differ...

Ngày tải lên: 23/03/2014, 09:21

... understand the structural basis of GluR -A SAP97 interaction, we have determined the crystal structure of SAP9 7PDZ2 in the presence and absence of a GluR -A C-terminal 18-mer peptide ligand The structure ... the wild-type CTD associated with the native SAP97 (Fig 1B) In vitro binding of GluR -A C-terminus to SAP97 PDZ domains To further examine...

Ngày tải lên: 30/03/2014, 10:20

... differences from the analogous structures of the ascomycete laccase (asco -laccase) MaL, thus giving a comprehensive view of the structure and function of asco-laccases Results and Discussion Overall structure ... Thornton JM (2003) Structural characterization and functional significance of transient protein–protein interactions J Mol Biol 325, 99 1–1 018 Crystal str...

Ngày tải lên: 14/02/2014, 18:20

... A2 for main-chain atoms, 35.7 A2 for side-chain ˚ overall (3 244 atoms) For the pepatoms and 33.8 A tide, B factors are slightly higher than those for ˚ ˚ importin -a: 36.9 A2 for main-chain atoms, ... atoms, 40 .2 A2 for ˚ overall (62 total atoms) side-chain atoms and 38.7 A Electron density analysis Both the main-chain and side-chain atoms of the modelled CLIC4 peptide...

Ngày tải lên: 14/02/2014, 19:20

... substrate recognition For the superposition of the substrate- free PhyK with substrate- free ˚ AppA the Ca atoms are 2.41 A apart, whereas for the substrate- free PhyK and the substrate- loaded AppA ... B Fig Crystal structure of Klebsiella sp ASR1 phytase (A) Cartoon representation showing the two domains of PhyK, the a domain (orange) and the a ⁄...

Ngày tải lên: 16/02/2014, 09:20

... 14-48 form the small insertion domain that comprises a short a- helix and a three-stranded anti-parallel b-sheet; the remaining protein residues form the (a ⁄ b)8 barrel domain and a C-terminal extension ... would act as a regulatory link between glycolysis and NAD synthesis The structure of hACMSD in complex with DHAP may used for the design o...

Ngày tải lên: 18/02/2014, 06:20

... References Ivanova N, Sorokin A, Anderson I, Galleron N, Candelon B, Kapatral V, Bhattacharyya A, Reznik G, Mikhailova N, Lapidus A et al (2003) Genome sequence of Bacillus cereus and comparative analysis ... of GAB catalysis have been identified to date [12] which are based either on a single, bifunctional GAB catalyst or on a GAB catalysts pair The available biochemical dat...

Ngày tải lên: 19/02/2014, 00:20

... the carboxyl oxygen atoms of the side chains of Asp11, Asp14 and Asp21, the amide oxygen atom of Gln15 and the carbonyl oxygen atoms of Asp11 and Asn23 (Fig 3) in an arrangement similar to what ... within a few days to thin hexa62 Table Data collection and refinement statistics for SPRK ˚ Resolution (A) Space group Cell parameters ˚ a- axis (A) ˚ b-axis (A) ˚...

Ngày tải lên: 19/02/2014, 07:20

... of cold adaptation ase structure, the first structure of a cold- adapted subtilase to be determined, enables a more focused examination of plausible determinants of different temperature adaptation ... Number of ion pairsa Number of hydrogen bonds Main chain–main chain Main chain–side chain Side chain–side chain Total ˚ Exposed surface areab (A2 ) ˚ Apolarc (A2 ) ˚ Bu...

Ngày tải lên: 19/02/2014, 16:20

... effect of these substrate analogues This is consistent with a simultaneous interaction of the 1,3BPGA analogues at both Ps and Pi phosphate-binding sites Comparison of the inhibition of T cruzi and ... specific binding modes of these 1,3-BPGA analogues to the two trypanosomatid enzymes Materials and methods Sources of substrates, cofactors and inhibitors The synthesis of 1,...

Ngày tải lên: 20/02/2014, 02:21