UNDERSTANDING ACTIN FUNCTION DURING CYTOKINESIS IN FISSION YEAST

... gain more knowledge about actin function during cytokinesis, new methods to track F -actin dynamics in vivo, reexamine myosin mutants during actomyosin ring assembly and isolation of novel actin ... available In all, fission yeast is a good model organism for studying cytokinesis 1.4 ACTIN CYTOSKELETON IN FISSION YEAST 1.4.1 Actin patches Actin patches are on...

Ngày tải lên: 10/09/2015, 09:25

... cycle regulation of cytokinesis in metazoans 19 1.3.2.2 Cell cycle regulation of cytokinesis in fission yeast 21 Spatial regulation of cytokinesis 23 1.3.3.1 Positioning of division plane in metazoans ... model organism 1.3 Cytokinesis 1.3.1 10 1.3.1.2 Assembly of the actomyosin ring in fission yeast 11 1.3.1.3 Signaling pathways invovled in cytoki...

Ngày tải lên: 11/09/2015, 09:06

... the function of the septation initiation network (SIN) These experiments established the fact that the MeiAR ensures proper targeting of the membrane biogenesis machinery to the leading edge, thereby ... in higher temperature In addition, there is no detailed analysis of the syntaxin protein Psy1p during the whole meiotic process in SIN mutant cells Thus,...

Ngày tải lên: 10/09/2015, 15:53

... PHOSPHOREGULATION OF THE ACTIN CYTOSKELETON DURING ENDOCYTOSIS IN THE YEAST SACCHAROMYCES CEREVISIAE JIN MINGJI A THESIS SUBMITTED FOR THE DEGREE OF DOCTOR OF PHILOSOPHY INSTITUTE OF MOLECULAR ... and endocytosis in the yeast Saccharomyces cerevisiae 1.4.1 Actin structures in yeast 1.4.2 Endocytosis in yeast 10 1.4.3 Actin involvement...

Ngày tải lên: 12/09/2015, 08:19

... tool for protein targeting to answer existing questions pertaining to fission yeast mitotic regulation and cytokinesis Several aspects need to be investigated regarding the positioning and assembly ... requirement of F-actin for cytokinesis in fission yeast (Balasubramanian et al., 1992) Additional isolation and analyses of fission yeast mutants defective in cyt...

Ngày tải lên: 09/09/2015, 11:21

... Wilhelm et al.: Differential patterns of intronic and exonic DNA regions with respect to RNA polymerase II occupancy, nucleosome density and H3K36me3 marking in fission yeast Genome Biology 2011 12:R82 ... there is decreasing H3K36me3 modification and increasing Pol II accumulation within intronic regions relative to exonic regions P...

Ngày tải lên: 09/08/2014, 23:20

... a dynamic interaction between vpr gene expression and activation of Hsp16 in fission yeast Results of our parallel studies in mammalian cells indicated a similar dynamic and antagonistic interaction ... Yu M, Chen M, Edelson S, Zhao Y: Cell cycle G2 arrest induced by HIV-1 Vpr in fission yeast (Schizosaccharomyces pombe) is independent of cell death...

Ngày tải lên: 13/08/2014, 09:20

... possible by extending a recently-introduced linearized biexponential model (LinBiExp) [21] to allow fitting of general bilinear- type data with a single, unified model Originally, LinBiExp was introduced ... modelof the length -growth in a representative WT fission yeast cell fitted with an exponential (Exp) and a bilinear (LinTime-profile Time-profile of the length -gro...

Ngày tải lên: 13/08/2014, 23:20

... meiotic splicing in fission yeast Mol Cell 2005, 18:491-498 Harigaya Y, Tanaka H, Yamanaka S, Tanaka K, Watanabe Y, Tsutsumi C, Chikashige Y, Hiraoka Y, Yamashita A, Yamamoto M: Selective elimination ... transcription factor containing a forkhead DNA-binding domain Mol Cell Biol 1998, 18:2118-2129 Iino Y, Yamamoto M: Negative control for the initiation of meiosis in Schizosacchar...

Ngày tải lên: 14/08/2014, 08:20

... copy number refereed research Figure of Stability wild-type strain gene expression profiles Stability of wild-type strain gene expression profiles (a) Microarray expression data for two wild-type ... Unknown/hypothetical Fifty-four genes with maintained expression changes twofold or more in both of strains C1 and C5 that also had changed expression of twofold or more dur...

Ngày tải lên: 14/08/2014, 14:21

... activation of Cdc42 in S pombe (Tatebe et al., 2008) Positioning and assembly of division plane in fission yeast Positioning of division plane in fission yeast In S pombe, the actomyosin ring is assembled ... role in regulating cell polarity 12 Cell end localized polarity factors 13 Positioning and assembly of division plane in fission yeast...

Ngày tải lên: 11/09/2015, 09:10

... cerevisiae S pombe Schizosaccharomyces pombe SAPK stress- activated protein kinase SREBP sterol regulatory element-binding protein Sty1p suppressor of tyrosine kinase protein TAG triacylglycerols ... group of biogenic amines that are neural transmitters, and include dopamine, norepinephrine and epinephrine (adrenaline) and glucagon are important inducers of lipolysis and HSL is...

Ngày tải lên: 16/09/2015, 15:55

... both a novel C-terminal actin- binding submodule (CABS) containing a novel actin monomer binding verprolin homology C-terminal (VH2-C) domain and a second submodule comprising the previously characterized ... actinbinding domain (this actin- binding domain has not yet been mapped) [23] We name the actin- binding domain that we have identified VH2-C and VH2-...

Ngày tải lên: 18/02/2014, 16:20

... by the degradation of the septum by chitinase and possibly other enzymes (Kuranda and Robbins 1991) 1.6 Cytokinesis in fission yeast 1.6.1 The fission yeast Schizosaccharomyces pombe The fission ... disassembly of the spot prevent actomyosin ring assembly, implying that the spots may be the precursor of the astomyosin ring (Wong et al 2002) Prior to th...

Ngày tải lên: 14/09/2015, 14:18

... both dlp1 and dps1 are essential for decaprenyl diphosphate synthase activity in S pombe Fig Thin-layer chromatogram of the product of decaprenyl diphosphate synthase The decaprenyl diphosphate synthase ... synthesis in the dlp1 mutant, as well as in the dps1 mutant, is due to the lack of decaprenyl diphosphate synthase activity We furth...

Ngày tải lên: 23/03/2014, 21:20