... particular These substrate analogs are related to the commercially available methylanthraniloyl (mant) derivatives of ATP and GTP commonly used in mechanistic studies of ATPases and GTPases Here, we ... separate the substrate and products [11] This assay has been instrumental in studying the mechanism of glucosylhydrolase activity, but has several disadvantages, including the discontinuous nature ... Ithaca, NY, USA, and A Bender, Indiana University, Bloomington, USA, respectively, and purified by affinity chromatography as previously described [19] All buffers were prepared from the appropriate...