Roles of long non coding RNAs in human embryonic stem cell pluripotency and neural differentiation 4

... was confirmed by qPCR in two independent cell lines: hESC (H1)-derived NPCs and an immortalized human neural stem cell line ReN-VM In both hESC-derived cells and ReN-VM cells, RMST expression ... silencing factor, is a transcription factor expressed in neural stem cells and non- neuronal cells, to repress neuronal gene expression To confirm that REST indeed binds up...

Ngày tải lên: 09/09/2015, 17:54

... specification of midbrain dopamine neurons Radial glia cells are neuronal progenitors in vivo 10 11 14 1. 3 Long non- coding RNAs in biology 15 1. 3 .1 1.3.2 1. 3.2 .1 1.3.2.2 1. 3.2.3 Long non- coding RNAs in pluripotency ... pluripotency, and repressing differentiation simultaneously   18   1. 3.2 Long non- coding RNAs in neural developmen...

Ngày tải lên: 09/09/2015, 17:54

Ngày tải lên: 09/09/2015, 17:54

... chr 13: 36 ,31 5,670 -36 ,31 7,9 83 chr4:1,179,572-1,181,6 03 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 AK124684 AK125262 AK12 539 2 AL 832 189 AY927 532 BC008027 BC 030 122 BC 031 955 ... location (NCBI36/hg18) AF242771 AK05 533 5 AK056826 (lncRNA_ES1) AK0911 13 AL117559 AL 833 138 BC02 630 0 (lncRNA_ES3) chr12: 63, 376, 238 - 63, 376,597 chr19: 43,...

Ngày tải lên: 09/09/2015, 17:54

... established in the mouse model system, and very few human lncRNAs have been studied with such extent In this thesis, I focused on examining lncRNAs involved in the pluripotency maintenance of human embryonic ... B., Liu, M., and Shi, T (2011) Comparative analysis of human protein -coding and noncoding RNAs between brain and 10 mixed cell lines by RNA-Seq PLoS...

Ngày tải lên: 09/09/2015, 17:55

... changed to DMEM/F12 (Invitrogen, 10 565-042) supplemented with Non-essential amino acids (Invitrogen, 11 114 0050), N2 (Invitrogen, 17 502-048), heparin (Sigma, H 314 9) and FGF-2 (ProSpec, CYT-557 _10 µg) ... harvested using collagenase and seeded onto CoStar non-adherent plates (Corning, 34 71) to form embryoid bodies using 20% Knockout Serum (Invitrogen, A1099202) in Knockout DME...

Ngày tải lên: 11/09/2015, 10:06

... IPI0 022 0 327 Extracellular Matrix Ribosome-binding protein 66 022 IPI0 029 7646 138 923 IPI003049 62 129 297 IPI00013079 IPI00 328 113 IPI00 329 573, IPI009645 52 IPI00 022 200, IPI000 729 18, IPI0 022 0701 IPI00 022 418, ... Christopher Stephen Hughes and Prof Gilles Lajoie * Protein also found in GFR and standard Matrigel, based on work by Hughes et al 20 09 † Murine protein...

Ngày tải lên: 11/09/2015, 10:06

... matrices contains more Fn (by immunofluoresence comparison, section 3. 3.2), it would be expected that the 52 increased presence of Fn would aid in increasing integrin binding and hence cell binding This ... This contradiction could possibly be due to the increased presence of DCN and BGN in the DxSNP40/DNase and FcNP40/DNase matrices interfering with TGFB signaling for the hESCs...

Ngày tải lên: 11/09/2015, 10:06

... gastrulation in an anterior-posterior pattern Germ cells continue to maintain the expression of Oct -4 until the initiation of sexual differentiation [10] The under-expression of Oct -4 in ES cells ... their differentiation into trophoectoderm lineages [10], indicating the crucial role that Oct -4 plays in maintaining the pluripotent state of ES cells As Oct -4 is a transcription fa...

Ngày tải lên: 11/09/2015, 10:07

... and were transferred onto laminincoated plates and incubated in neural induction medium Under these conditions, hESCs were able to form extensions between embryoid bodies that were stained positive ... that maintain hESC pluripotency using dispase 3.7.1 Population doublings In section 3.4, 3 .5 and 3.6, all hESCs were passaged by enzymatic passaging using collagenase IV There is an...

Ngày tải lên: 11/09/2015, 10:07

... collagen deposition: Fab’ to the gelatin binding domain of fibronectin inhibits both fibronectin and collagen organization in fibroblast extracellular matrix J Cell Biol 92:485-492 (1982) 52 Velling ... amount of a beneficial substance might have adverse effects Indeed, it has been reported that MEFCM contains BMP-antagonists, noggin and gremlin, and hESCs can be maintained in...

Ngày tải lên: 11/09/2015, 10:07

... (see Yfr2, Yfr3, Yfr4 Comparison offollowing page) and Yfr5 from MED4 Comparison of Yfr2, Yfr3, Yfr4 and Yfr5 from MED4 (a) Sequence comparison of the yfr2 through yfr5 coding regions of MED4 Transcriptional ... many bacterial regulatory RNAs is stimulated by varying environmental cues, and often so by the stress response in which these RNAs then play a role Therefore, a variety...

Ngày tải lên: 14/08/2014, 14:22

... technology to quantify the expression of non-coding small RNAs, including miRNAs and piRNAs/21U -RNAs, and demonstrate dynamic and sex-specific expression pattern changes during development of C elegans ... Figure Validation of the expression of novel miRNAs Validation of the expression of novel miRNAs (a) Validation of the expression of novel...

Ngày tải lên: 14/08/2014, 21:20

... catabolic and inflammatory pathways that take place during OA by inducing the synthesis of major catabolic and inflammatory mediators via the p38 and p 42/ 44 signalling pathways Furthermore, PAR -2 upregulation ... study design, acquisition of data, analysis and interpretation of data, statistical analysis, and manuscript preparation NA participated in acquisitio...

Ngày tải lên: 09/08/2014, 10:22