Vector design for monoclonal antibody production using chinese hamster ovary cells
Vector design for monoclonal antibody production using chinese hamster ovary cells
... biochemically similar to human forms for increased product efficacy and safety (Matasci et al 2009) 2.3.1 Chinese hamster ovary cells Chinese hamster ovary (CHO) cells were first isolated in 1958 ... Mammalian cells like the Chinese hamster ovary (CHO) cells are commonly used for their ability to perform the required protein modifications for product safety and...
Báo cáo Y học: Tyrosine sulfation and N-glycosylation of human heparin cofactor II from plasma and recombinant Chinese hamster ovary cells and their effects on heparin binding pot
... modifications on heparin binding, recombinant CHO cells were treated with inhibitors of tyrosine sulfation and N-glycosylation under conditions that allowed partial inhibition of these modifications ... In summary, we present evidence for a very strong similarity of HCII from human serum and its recombinant counterpart from CHO cells with respect to tyrosine...
Báo cáo khoa học: "Biological characteristics of Chinese hamster ovary cells transfected with bovine Prnp" pptx
... noisnetxe lanif a neht dna ,ces 03 nim rof Co27 ta noisnetxe ,ces 03 rof Co06 ta gnilaenna ,ces 03 rof Co49 ta noitarutaned fo selcyc 03 yb dewollof ,nim rof Co49 ta noitarutaned laitini na fo ... slleC noitatipicerponummI enivob fo noitpircsnart eneg rof neercs ot evoba debircsed sa demrofrep neht saw RCP dna ,ANR evomer ot nim 02 rof Co73 ta H esaNR fo stinu htiw detaert erew ANDc dednarts-...
monoclonal antibody production
... Large-Scale Production of Monoclonal Antibodies 25 Monoclonal Antibody Production for Diagnostic and Therapeutic Purposes 25 In Vivo and In Vitro Methods for Commercial Production of mAb 26 In Vivo Production ... Secreting Monoclonal Antibodies In Vitro Production of Monoclonal Antibody 12 Batch Tissue-Culture Methods 12 Semipermeable-Membrane-Based Systems 14 Scientific...
Báo cáo y học: " Evaluation of anti-IL-6 monoclonal antibody therapy using murine type II collagen-induced arthritis" pptx
... influence of IL-6 in arthritis, by examining the effects of anti-IL-6 mAb treatment in a murine model of type II collagen induced arthritis (CIA) Methods Induction and Assessment of Type II Collagen-induced ... mAb B: Anti-IL-6 (1mg/week) C: Anti-IL-6 (5mg/week) Figure Anti-IL-6 antibody reduced the inflammatory histopathology of CIA Anti-IL-6 antibody redu...
Báo cáo hóa học: " Plaque assay for human coronavirus NL63 using human colon carcinoma cells" ppt
... whether hCoV -NL63 might have adapted to CaCo-2 cells during plaque purification Conclusion Figure overlays3 Plaque assay for hCoV -NL63 on CaCo-2 cells using different Plaque assay for hCoV -NL63 on ... solution As shown in Figure 3, plaques were Figure Cytopathogenic effect of hCoV -NL63 on human colon carcinoma cells (CaCo-2) Cytopathogenic effect of hCoV -NL63 o...
SảN XUấT PROTEIN ERYTHROPOIETIN THÔNG QUA QUá TRìNH CHUYểN GEN TRÊN Tế BàO CHO - K1 (CHINESE HAMSTER OVARY)
... chuyển gen v protein tái tổ hợp tiến hnh hớng nghiên cứu nuôi cấy dòng tế bo CHO- K1 để chuyển gene, sau chọn lọc quần thể tế bo CHOK1 sản xuất EPO ổn định Tế bo CHO- K1 đợc chọn lm tế bo chủ chúng ... - Tế bo CHO- K1 (ATCC CCL 61) - Hóa chất dùng cho chuyển gene ExGen 500, PCR, điện di, ELISA hãng Sigma, Merck, Fermentas, Invitrogen đợc pha tùy theo...
Báo cáo khoa học: Okadaic acid induces DNA fragmentation via caspase-3-dependent and caspase-3-independent pathways in Chinese hamster ovary (CHO)-K1 cells pot
... caspase-3 is involved in OA-induced DNA fragmentation independently from DEVDase activity, and that DEVDase or DEVDase-like activity is involved in DNA fragmenta- Involvement of caspase-3 in DNA fragmentation ... cells In the case of caspase-3) ⁄ ) cells, DEVDase or DEVDase-like activity may play an important role in inducing DNA fragmentation OA induces caspase...
Translatomics study of chinese hamster ovary cell cultures
... Batch cultures 49 3.1.4 Feeding of batch cultures 49 3.1.5 Rapamycin treatment of batch cultures 50 3.1.6 Determination of cell viability 50 3.1.7 Determination of ... Background Chinese hamster ovary (CHO) cells are one of the most commonly used mammalian host cell lines by biopharmaceutical industries for the production of recombinant proteins of therapeutic ....
BT 3 optimization of medium for indole 3 acetic acid production using pantoea agglomerans strain PVM
... Biosynthesis of indole -3- acetic acid (a) Enterobacter sp DHM-1T (FJ74 530 0·1) Enterobacter sp R4M-Q (GQ478271·1) Pantoea agglomerans strain P29 (DQ3569 03 1) Pantoea agglomerans strain PVM (GU929212·1) Pantoea ... concerned with the IAA production potential of P agglomerans strain PVM, optimization of 1240 medium components and in vitro root induction...
Tài liệu Module 3: Using a Conceptual Design for Data Requirements docx
... Services and Data Models Module 3: Using a Conceptual Design for Data Requirements Module 4: Deriving a Logical Data Design Module 6: Deriving a Physical Data Design Module 5: Normalizing the Logical ... the Logical Data Design Investigation Process Module 3: Using a Conceptual Design for Data Requirements Activity 3.2: Relating Dat...
Tài liệu Instructor Notes Module 3: Using a Conceptual Design for Data Requirements pdf
... 2 Instructor Notes Module 3: Using a Conceptual Design for Data Requirements Activities Activity 3.1: Identifying Data- Related Use Cases and Data Requirements In this activity, students ... be able to: • Discuss use cases, data requirements, and requirements validation as they relate to conceptual design for data systems ! To prepare for the activity...
Báo cáo khoa học: Production of a recombinant mouse monoclonal antibody in transgenic silkworm cocoons pptx
... residues in the N-glycans attached to the mAb, SDS ⁄ PAGE with lectin blotting using Aleuria aurantia lectin (AAL) or concanavalin A was performed on the purified recombinant mAb and standard human ... strain pnd-w1 was obtained from the National Institute of Agrobiological Science (Tsukuba, Japan) The larvae were reared at 25 °C on an artificial diet (Silk Mate PM, Nosan Corp, Kanagawa...
Báo cáo khoa học: Novel strategy for protein production using a peptide tag derived from Bacillus thuringiensis Cry4Aa pptx
... or more replicate experiments A 6xHis 4AaCter-TpN15 PP TpN15 B TpN17 4AaCter 4AaCter 4AaCter-TpN17 4AaCter 4AaCter-TpN47 4AaCter TpN47 4AaCter 4AaCter TpN15 TpN17 TpN47 ATG Ptac (kDa) 210 119 90 ... (Invitrogen, Carlsbad, CA, USA) and incubated with PreScission protease at a concentration of UÆ100 lg)1 protein to remove the · His–4AaCter tag The released · His–4AaCter tag and undige...
Design for network: Using the Internet
... LINUX, AND WIKIPEDIA FOR GRANTED #DESIGNFORNETWORKS // @MIKEARAUZ // MAY 2010 WHAT THE INTERNET IS GOOD FOR: AWARENESS PERSUASION SHARING COOPERATION COLLECTIVE ACTION #DESIGNFORNETWORKS // @MIKEARAUZ ... NETWORK #DESIGNFORNETWORKS // @MIKEARAUZ // MAY 2010 THINGS USED TO BE SIMPLE #DESIGNFORNETWORKS // @MIKEARAUZ // MAY 2010 THINGS GOT DIGITAL #DESIGNFORNETWORKS // @MIKEARAUZ // MAY 2...
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