THE MECHANISM OF PPARN3 MEDIATED DOWN REGULATION OF SODIUM HYDROGEN EXCHANGER 1 (NHE1) GENE EPXRESSION AND ITS INHIBITION BY ESTROGEN RECEPTOR n1 2

... mgÆmL)1 BSA and stored at )20 °C Electrogenic BSP uptake inhibition by antibodies The kinetics of bilitranslocase transport activity inhibition by antibodies were examined by preincubating 24 lL ... addition of protein (2.6 ± 0.04 lmolÆmin)1Æmg protein)1, with lg valinomycin), as well as of K+ (6.25 ± 0.16 unitsÆmEq)1 K+, with lg valinomycin) and valinomycin (0.51 ± 0.03 unitsÆlg)1 valinomycin, ... also investigated in carnation petal microsomes by preincubating them with antibody A in the presence of increasing concentrations of bilirubin BSP uptake was assayed to track the progress of the...

... Endocrine/Explorations/Endocrine Function Chapter 11 1 1- 1 Muscular/Anatomy/Skeletal Muscle 1 1- 3 Muscular/Histology/Cardiac Muscle Muscular/Histology/Smooth Muscle xxvi 1 1- 4 1 1- 5 1 1- 6 1 1- 8 1 1- 1 2 1 1- 1 5 1 1- 1 8 1 1- 1 9 1 1- 2 0 1 1- 3 1 ... Tape Tape Chapter 11 1 1- 8 Tape 1 1- 1 2 Tape 1 1- 1 6 Tape Chapter 15 1 5-6 Tape 1 5-8 Tape 1 5-2 5 Tape Chapter 16 1 6-6 Tape 1 6 -1 4 Tape Chapter 6 -1 6 -1 2 6 -1 8 6 -1 9 1 0-2 Tape 1 0-7 Tape 1 1 Module Module Module ... Chapter 12 1 2-2 Nervous/Exploration/Motor and Sensory Pathways 1 2-8 Nervous/Explorations/Reflex Arc Chapter 13 1 3 -1 5 Nervous/Anatomy/Gross Anatomy Chapter 14 1 4 -1 1 4-7 1 4-8 1 4 -1 2 1 4 -1 4 1 4 -1 5 1 4 -1 6 1 4-2 0...

... Tat1 and Tat2 RNAs in the untreated RRL system Translation of Tat1 and Tat2 RNAs in the untreated RRL system The top three lanes depict the Glob-Fluc, UTRTat1-Rluc and UTRTat2-Rluc RNAs used in the ... one of the DNA fragments corresponding to the complete 5' UTR of Tat1 and Tat2 RNAs, to the deleted 5' UTR (delta R-U5) of Tat1 and Tat2 RNAs, to the 5' UTR of the viral genomic RNA, and to the ... http://www.retrovirology.com/content/6/1/74 Figure Translation of the Tat RNA in the RRL system Translation of the Tat RNA in the RRL system A Structure of the recombinant Glob- and EMCV RNA templates...

... RGYVYQGL (VSV8) and physiological low-molecular mass ligands of the ER lumenal compartment (Ca2+ and ATP) , known to regulate the function of Grp94 as a protein chaperone [4,11,12] Thus, the SPR binding ... ligands of the chaperone in the ER lumenal compartment which may perturb this interaction Binding of the viral immunogenic octapeptide VSV8 to native rGrp94 Native glycosylated rat Grp94 (rGrp94) and ... low-affinity binding sites for VSV8 Binding of ATP, MgATP and NECA to rGrp94 The function of Grp94 as a protein chaperone is reported to be sensitive to ER lumenal ATP levels [11] The chaperone...

... exchanger- 1 (NHE1) 1. 1 .1 NHE1 isoforms and NHE1 structure 1. 1.2 Regulation of NHE1 1. 1.3 Physiological function of NHE1 1. 1.4 Role of NHE1 in cancer progression 11 ... formation of Hsp90/Akt/NHE1 complex in MCF10AT1 12 4 3.3.3 Akt regulates NHE1 protein expression in MCF10CA1a 13 1 3.3.4 NHE1 interacts with Akt in MCF10CA1a and not MCF10CA1h 13 4 3.3.5 ... 14 4 CHAPTER 4: DISCUSSION 15 1 4 .1 NHE1 is a potential client protein of Hsp90 in MEFPTEN-/- cells 15 1 4 .1. 1 Increased stability of NHE1 protein in MEFPTEN-/- cells in comparison...

... REGULATION OF NHE1…………… 11 0 3B .1 DOWN -REGULATION OF NHE1 BY H2O2 11 0 3B .1. 1 Identification of crucial region in the promoter of NHE1 11 0 3B .1. 2 H2O2 down-regulates 0 .15 kb proximal NHE1 promoter ... signaling 21 1.3.4 Regulation of estrogen receptors 23 1. 3.5 Estrogen and estrogen receptors in human breast cancer 27 v 1. 4 THE SODIUM HYDROGEN EXCHANGER 29 1. 4 .1 pH regulation ... turnover 16 1. 3 ESTROGEN RECEPTOR 17 1. 3 .1 Estrogen and estrogen receptors: Introduction 17 1. 3.2 Estrogen receptor and genomic signaling 20 1. 3.3 Membrane and cytoplasmic...

... CGCGGATCCAATGGACTACAAAGACGATGAC GACAAGAGCATAGTGATCCCA MLL5β M5b_NotI.rev AAGGAAAAAAGCGGCCGCCAATATACGCGA GACTAGTCTT GFPMLL5β M5b_SalI.for ACGCGTCGACATGAGCATAGTGATCCCATTG M5b_BamHI.rev CGCGGATCCCAATATACGCGAGACTAGTCTT ... HPV18_7239.rev ACAACAACAACCATACATACC HPV18_ 7168 .for TGTTGTGTTTGTATGTCCTGT HPV18_7 350 .rev CCACAAACACAAATACAGTTGTT HPV18_7378.for TATTGTCCTGTATTTCAAGTTAT HPV18_ 757 6.rev CGCGCCAATTGTTCAAAATATG 2.11 Rapid amplification ... CATCTGACATATTTTCCCGCTTCCGGCGTTGT AGTAGCAC 36    GFP- M5_Y 35 8A. for AGAGGGAAGTTTATG MLL5β‐ SET mut ATTTGCCTCCTGATGCACTTATCATTGAAGCC M5_Y 35 8A. rev CATAAACTTCCCTCTGGCTTCAATGATAAGTG CATCAGGAGGCAAAT...

... phosphorylation of GST–TSSK3 with [32 P]ATP[cP] The phosphorylation reactions of GST–TSSK3K39R by Myc-PDK1 [32 ] immunoprecipitated from 293T cells and of HA–TSSK3K39R immunoprecipitated from 293T cells, by ... maximum activation and ⁄ or activation by PDK1 of TSSK3, as suggested for PKCf phosphorylation and activation by PDK1 [35 ] Thus we hypothesize that in order to efficiently recruit PDK1 to TSSK3, cofactors ... the regulation of TSSK3 activity and suggest a similar mechanism of activation to that of the AGC kinase family For a number of AGC kinases the 3- phosphoinositide-dependent protein kinase- 1 (PDK1)...

... isonicotinoyl hydrazone class as effective antiproliferative agents II The mechanism of action of ligands derived from salicylaldehyde benzoyl hydrazone and 2-hydroxy-1-naphthylaldehyde benzoyl hydrazone ... controls Apolactoferrin was from Calbiochem A polyclonal rabbit anti-(human ferritin) Ig was from Roche Diagnostics All other chemicals were of analytical reagent quality The NO- generators and other ... identifying the exact molecular nature of the Fe released by NO and the transporter involved are underway Finally, out current results may be important in understanding the cytotoxic actions of NO...