Novel non apoptotic pathway of caspase 3 activation during mild oxidative stress
Novel non apoptotic pathway of caspase 3 activation during mild oxidative stress
... of non- apoptotic functions of caspases 13 1.2 Oxidative stress and caspase activation 17 1.2.1 Oxidative stress 17 1.2.2 ROS-mediated caspase activation 19 1.2 .3 Caspase ... 33 CHAPTER RESULTS 34 3. 1 Characterization of non- classical caspase activation upon H2O2 treatment 34 3. 1.1 Exposure of L6 myoblasts to non- toxic does of H2O2 results in...
Ngày tải lên: 09/09/2015, 10:14
Báo cáo y học: "Inhibition of TNFalpha in vivo prevents hyperoxia-mediated activation of caspase 3 in type II cells" pdf
... deficiency Free Radic Biol Med 2001, 30 :1145-11 53 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 Sinha P, Kolleck I, Volk HD, Schlame M, Rüstow B: Vitamin E deficiency sensitizes alveolar type II ... respiratory distress syndrome [ 13] , and hyperoxia-mediated increase of total lung p 53 protein expression [14] alveolar type II cells (TIIcells) are severely damaged...
Ngày tải lên: 12/08/2014, 18:21
Báo cáo khoa học: Interaction of caspase-3 with the cyclic GMP binding cyclic GMP specific phosphodiesterase (PDE5a1) potx
... role in the binding of competitive PDE4 inhibitors [36] The side chain of this residue may conceivably p-stack with the purine base of the bound substrate and form hydrophobic interactions with ... cGMP phosphodiesterase can modulate the proteolysis of two cGMP binding cGMP -specific phosphodiesterases (PDE6 and PDE5) by caspase-3 Cell Signal 13, 735–741 Aravind,...
Ngày tải lên: 17/03/2014, 09:20
Báo cáo khoa học: Characterization of ubiquitin-like polypeptide acceptor protein, a novel pro-apoptotic member of the Bcl2 family pptx
... Tanigawa, Y (1995) Molecular cloning and characterization of a cDNA encoding monoclonal nonspecific suppressor factor Proc Natl Acad Sci USA 92, 3463–3467 10 Nakamura, M., Xavier, R.M & Tanigawa, ... of the 33.5 kDa Ubi-L adduct The 33.5 kDa Ubi-L adduct was digested by V8 protease and subjected to MALDI-MS analysis The data in the second column are the mass values obtained e...
Ngày tải lên: 17/03/2014, 10:20
Báo cáo y học: "Elevated expression of caspase-3 inhibitors, survivin and xIAP correlates with low levels of apoptosis in active rheumatoid synovium" potx
... between survivin and cleaved caspase-3 expression Additionally, our finding that the mRNAs of caspase-3, xIAP and survivin were generally higher in active RA compared with inactive RA synovial ... related apoptosis inducing ligand; TUNEL = terminal deoxynucleotidyl transferase biotin-dUTP nick end labelling; xIAP = x-linked inhibitor of apoptosis protein levels...
Ngày tải lên: 09/08/2014, 01:22
báo cáo khoa học:" Expression of caspase-3, p53 and Bcl-2 in generalized aggressive periodontitis" ppsx
... fashion using a scale of to 3+: (0) = no staining; (1+) = stained cells comprising >10% of the inflammatory infiltrate (2+) = stained cells comprising 30% of the inflammatory infiltrate, and (3+) ... hematoxylin Colon adenocarcinoma (for p53) and tonsil tissue (for Bcl-2) were used as positive controls for immunostaining Levels of expression of p53 and Bcl-2 on each sl...
Ngày tải lên: 11/08/2014, 23:22
Báo cáo y học: " Inhibition of caspase-1 activation in gram-negative sepsis and experimental endotoxemia" ppsx
... stages of sepsis in a similar way as proinflammatory cytokines differed These findings led us to hypothesize that inhibition of ex vivo cytokine release by PBMCs in clinical sepsis is modulated in ... cytokines during sepsis is that transcription of proinflammatory cytokines is reduced Therefore, the number of RNA transcripts of TNFa and of IL-1b in the cell lysa...
Ngày tải lên: 14/08/2014, 07:21
Báo cáo khoa học: Increased expression of c-Fos by extracellular signal-regulated kinase activation under sustained oxidative stress elicits BimEL upregulation and hepatocyte apoptosis pot
... levels of c-Fos, c-Jun, or BimEL, and did not affect hepatocyte apoptosis (Figs and 5) These results indicate that c-Fos and c-Hun are crucial for BimEL expression and induction of hepatocyte apoptosis ... Ishihara et al Regulation of BimEL expression by c-Fos A (a) (b) B C (c) (a) (b) Fig Suppression of BimEL expression by knockdown of c-Fos...
Ngày tải lên: 06/03/2014, 00:21
Báo cáo khoa học: "Comparative study of PM2.5 - and PM10 - induced oxidative stress in rat lung epithelial cells" pptx
... mRNA induced by Comparative study of PM2.5 - and PM10 - induced oxidative stress in rat lung epithelial cells 15 Fig RT-PCR analysis of catalase mRNA The level of catalase mRNA induced by PM2.5 ... and Comparative study of PM2.5 - and PM10 - induced oxidative stress in rat lung epithelial cells 13 TTGTTTCTCGT-3', MTH1...
Ngày tải lên: 07/08/2014, 17:22
Regulation of na+ h+ exchanger 1 (NHE 1) gene expression by mild oxidative stress
... Regulation of activity and expression of NHE 1 54 1. 4.4 A 1. 4.4 B 1. 5 Regulation of NHE -1 activity 54 Transcriptional regulation of NHE -1 expression .58 AIM OF STUDY 61 ... response repression of NHE -1 expression .11 1 Figure 13 : Thiol oxidizing agent diamide mimicked the effect of H2O2 in the downregulation of NHE -1 gene expression 11 3 Figu...
Ngày tải lên: 14/09/2015, 08:50
The roles of shca proteins in response to oxidative stress 1
... separated to the nuclear and cytoplasmic fractions The levels of FOXO3a, TopBP1 (nucleus control), and p6 6shcA (cytoplasm control) were determined by western blot Note that four fold more total proteins ... using anti-phospho-tyrosine antibodies (C) Point mutation analysis to identify the tyrosine residues that was phosphorylated in the presence of H O p5 2shcA carrying...
Ngày tải lên: 14/09/2015, 14:17
The roles of shca proteins in response to oxidative stress 2
... for in vitro kinase assay as described in Fig 4.5A -1 02- A p-Erk1 /2 Erk1 /2 H2O2(mM) 0.1 0 .2 0.4 0.5 pMSCVp52ShcWT 0.1 0 .2 0.4 0.5 pMSCVp52ShcS29A B p 52 p46 pMSCVp52S29A pMSCVp52WT ShcA- /- ShcA ... concentrations of H2O2 ERK activation was analyzed with Western blot (B) Expression of p5 2ShcA or p5 2ShcA S29A in retroviral infected MEFs -104- A P-Erk1 /2 Erk1 /2 H2...
Ngày tải lên: 14/09/2015, 14:18
Tài liệu Báo cáo khoa học: 3T3-L1 adipocyte apoptosis induced by thiazolidinediones is peroxisome proliferator-activated receptor-c-dependent and mediated by the caspase-3-dependent apoptotic pathway doc
... PPARc agonist -induced apoptosis [26,27] In the present study, we investigated PPARc agonist -induced adipocyte apoptosis by using 3T3-L1 adipocytes and rat primary adipocytes Adipocyte apoptosis could ... troglitazone -induced adipocyte apoptosis is probably mediated by PPARc, GW9662 should have an inhibitory effect on adipocyte apoptosis As shown in Fig 2B...
Ngày tải lên: 16/02/2014, 09:20
Tài liệu Báo cáo khoa học: A novel coupled enzyme assay reveals an enzyme responsible for the deamination of a chemically unstable intermediate in the metabolic pathway of 4-amino-3-hydroxybenzoic acid inBordetellasp. strain 10d doc
... metacleavage pathway of 2-aminophenol in Pseudomonas sp strain AP-3 (A) Proposed pathway of 4-amino-3-hydroxybenzoic acid in Bordetella sp strain 10d (10) I, 4-amino-3hydroxybenzoic acid; II, 2-amino-5carboxymuconic ... of a mutant, strain Y-2, of the aniline-assimilating Pseudomonas sp strain AW-2 [20] Results Spectral changes during metabolism of 4-amino...
Ngày tải lên: 19/02/2014, 16:20
An american – vietnamese cross-cultural study on non-verbal expressions of disappointment part 3
... AN AMERICAN – VIETNAMESE CROSS-CULTURAL STUDY ON NONVERBAL EXPRESSIONS OF DISAPPOINTMENT ABSTRACT The purpose of this study was to investigate the most common nonverbal expressions of disappointment ... CHAPTER 3: DATA ANALYSIS AND DISCUSSION OF THE FINDINGS .21 3. 1 DATA ANALYSIS: 21 3. 1.1 Most common expressions of disappointment used by Vietnam...
Ngày tải lên: 07/11/2012, 15:07