Human pluripotent stem cell derived cellular vehicles for cancer gene therapy

... Ng W H , Wang S 4 .Human embryonic stem cell- derived mesenchymal stem cells as cellular delivery vehicles for prodrug gene therapy of glioblastoma Human gene therapy , Volume 22 , Issue 11 (2011) ... Pluripotent stem cells 19 20 20 1.2.1.1.1 Embryonic stem cells 20 1.2.1.1.2 Induced pluripotent stem cells 21 1.2.1.2 Adult stem cells 23 1.2.1.2.1 Mesenchyma...

Ngày tải lên: 09/09/2015, 10:08

... DEVELOPMENT OF NEW HUMAN STEM CELLDERIVED CELLULAR VEHICLES FOR GLIOMA GENE THERAPY ZHAO YING (B Sc., PKU; M Sc., PKU) A THESIS SUBMITTED FOR THE DEGREE OF DOCTOR OF PHILOSOPHY DEPARTMENT OF ... stem cells by adherent monoculture 4.3.2 “Stemness” of human embryonic stem cell- derived neural 127 stem cells 4.3.3 In vitro glioma tropism evaluation o...

Ngày tải lên: 11/09/2015, 09:00

... HUMAN EMBRYONIC STEM CELL- DERIVED NEURAL STEM CELLS: DERIVATION, DIFFERENTIATION AND MICRORNA REGULATION KWANG WEI XIN TIMOTHY (B.Sc (Hons), NUS) ... mechanisms of regulation of NSC differentiation 1.1.5.2 Pluripotent stem cell- derived NSCs Human embryonic stem cells (hESCs), which are pluripotent cells derived from the inner cell mass of blast...

Ngày tải lên: 10/09/2015, 09:08

... enables them to serve as "Trojan horses" for the delivery of genes for cancer therapy Indeed, clostridial spores that were genetically manipulated to harbour genes for cancerstatic factors, prodrug ... these vectors (Table 1) Advantages of clostridial spores as "trojan horse" vectors for cancer gene therapy At present, there are various gene therapy vector sy...

Ngày tải lên: 14/08/2014, 19:22

... Guidelines for Human Embryonic Stem Cell Research http://www.nap.edu/catalog/11278.html GUIDELINES FOR HUMAN EMBRYONIC STEM CELL RESEARCH Committee on Guidelines for Human Embryonic Stem Cell ... rights reserved Guidelines for Human Embryonic Stem Cell Research http://www.nap.edu/catalog/11278.html 16 Guidelines for Human Embryonic...

Ngày tải lên: 06/03/2014, 15:20

... with an intra-peritoneal injection of 400 mg/kg chloral hydrate (Pharmaceutical Plant of Tiantan Hospital, Beijing, China) The rectal temperature was monitored and maintained at 37.5°C A scalp incision ... infarcted brain parenchyma of transplanted rats (A- iii and A- iv) A comparable extent of class II MHC was noted in ischemic rats irrespective of any therapy but unrema...

Ngày tải lên: 18/06/2014, 16:20

... and enrichment of cardiomyocytes derived from human embryonic stem cells Circ Res 2002, 91:501-508 He JQ, Ma Y, Lee Y, Thomson JA, Kamp TJ: Human embryonic stem cells develop into multiple types ... Research and Therapy 2008, 5:1 demonstrated the ability of hES cells to differentiate into a myriad of cell types that include neurons, hematopoietic cells, cardiomy...

Ngày tải lên: 10/08/2014, 05:20

... culture, expansion and manipulation of human pluripotent stem cells The culmination of two events has enabled the production of human embryonic stem cells: the birth of the first IVF test-tube baby ... summarizing 12 years of our team’s experience, skill and knowledge in the derivation, culture and expansion of human embryonic stem cells, and more recently, of...

Ngày tải lên: 22/03/2014, 09:20

... conservation and variability of DNA methylation across different stem cell lines A recent analysis of about 1% of the genome of HESC lines shows that, by monitoring DNA methylation and gene expression, ... (BS-seq) [21], we have recently determined the DNA methylation profile of the human embryonic stem cell line HSF1 [22] BS-seq is able to generate genom...

Ngày tải lên: 09/08/2014, 23:20

... article as: Tsai et al.: Overexpression of hTERT increases stemlike properties and decreases spontaneous differentiation in human mesenchymal stem cell lines Journal of Biomedical Science 2010 ... such as Oct4 and Nanog is associated with differentiation of neural stem cells, hematopoietic stem cells and MSCs However, an increase in Oct4 expressio...

Ngày tải lên: 10/08/2014, 05:21

... Nardi NB: In search of the in vivo identity of mesenchymal stem cells Stem Cells 2008, 26:2287-2299 Caplan AI, Bruder SP: Mesenchymal stem cells: building blocks for molecular medicine in the 21st ... batched) by cytospins and direct cell counts [33] BAL level of cytokines (R&D Systems, Minneapolis, MN) were assessed by ELISAs and Luminex multiplex assays as previously descr...

Ngày tải lên: 11/08/2014, 03:20

... mesenchymal stem cells; HD: hematopoietic differentiated cells; AIDS: acquired immune deficiency syndrome; HAART: highly active anti-retroviral therapy; HPCs: hematopoietic progenitor cells; ... established as to how these mesenchyme derived cells become susceptible to HIV-1 and whether their HIV-1 production rates are comparable to that observed in HIV-1 infected l...

Ngày tải lên: 13/08/2014, 01:20

... Expression of genes in the BMP2+ cells associated with plasticity, and mesodermal and NCSC phenotypes BMP2+ cells are still in a state of plasticity BMP2+ cells significantly upregulate Oct4 and Nanog ... 50 µm 20 µm Analysis neural crest stem cell associated transcripts in BMP2+ cells Figure of Analysis of neural crest stem cell associated transcripts in...

Ngày tải lên: 14/08/2014, 08:20