FUNCTION AND REGULATION OF CALCIUM INDEPENDENT PHOSPHOLIPASE a2 IN THE ATTENUATION OF PAIN IN MICE

... sometimes 2.1 Orofacial pain Orofacial pain is defined as pain within the structures of the oral cavity and face, usually of a diffuse pattern” (Zwemer, 1998) Orofacial pain includes pain disorders ... revealed that pain is one of the main reasons that patients look for medical attention and that the lifetime prevalence of pain indications such as back pain,...

Ngày tải lên: 09/09/2015, 08:15

... is the cessation of blood flow to the brain and disruption of metabolic demand During early stages of ischemia (within min), inositolcontaining glycerophospholipids are the main source of the increase ... define the physiological role of sPLA2 and ascertain its contribution in the inflammatory process 1.2 cPLA2 isoforms The cPLA2 family consists of six intr...

Ngày tải lên: 11/09/2015, 09:58

... iPLA2-VIA mediates U937 cell proliferation M A Balboa et al cPLA2 activity (% hydrolysis) A 0.0 0.2 0.4 0.6 0.8 1.0 [Pyrrophenone] (µM) B Number of cells × 105 iPLA2-VIA by treatment of cells ... the Ca2+dependent PLA2 activity of U937 cell homogenates The cell membrane assay was utilized (B) Time course of the effect of pyrrophenone on the proliferative capacity of U...

Ngày tải lên: 18/02/2014, 17:20

... increased rate of arachidonatephospholipid remodeling Lack of involvement of group IV and group VI phospholipase A2 in remodeling and increased susceptibility of proliferating T cells to CoA-independent ... concentrations that involves the direct acylation of both the sn-1 and sn-2 positions of PtdIns 1,2-Diarachidonoyl-glycerophosphoinositol Results Initial incorporation o...

Ngày tải lên: 23/03/2014, 06:20

... in protein protein interaction are blue and the dehydrogenase domains of Tic3 2 and Tic6 2 are shown in green Tic1 10 contains two transmembrane domains at the proximal N-terminus The topology of ... surprising to find proteins with redox-active domains as Tic constituents Up to now, the ‘regulon’ of the Tic complex comprises three proteins: Tic6 2, Tic3 2...

Ngày tải lên: 07/03/2014, 03:20

... distribution of FEBS Journal 278 (2011) 319 0–3 203 ª 2011 The Authors Journal compilation ª 2011 FEBS 3193 Function and regulation of ABCA1 K Nagao et al Table Effects of mutations on the functions of ABCA1 ... re-secreted The internalized ABCA1 and apoA-I were reported to co-localize within late endosomes, and ABCA1 rapidly 3194 Fig Membrane meso-domain orga...

Ngày tải lên: 22/03/2014, 15:21

... characterized is the Cullin RING E3 ubiquitin ligases 1.5 Cullin RING E3 Ubiquitin Ligases Cullins form an evolutionarily conserved gene family They were first discovered as mediators of ubiquitin dependent ... promoting the binding of CAND1 to the CRL complex (Cope and Deshaies, 2003; Bosu and Kipreos, 2008) CAND1 only binds to the unneddylated form of...

Ngày tải lên: 10/09/2015, 09:04

... MOLECULAR FUNCTION AND REGULATION OF THE BAX- ASSOCIATING PROTEIN MOAP- 1 Fu Nai Yang INSTITUTE OF MOLECULAR AND CELL BIOLOGY NATIONAL UNIVERSITY OF SINGAPORE 2007 MOLECULAR FUNCTION AND REGULATION ... pore 35 1. 3.3.3 Channel formation 38 ii 1. 4 THE MULTIDOMAIN PRO-APOPTOTIC PROTEIN BAX AND BAK 41 1.4 .1 Bax plays dominant role over Bak 41...

Ngày tải lên: 14/09/2015, 12:54

... cytosolic and membranebound PtdEtn-PLD activity Cytosolic and membrane-bound fractions were prepared as described in Materials and methods PtdEtn-PLD activity measured without addition of EDTA, ... characterized the cytosolic and membrane-bound forms of yeast PtdEtn-PLD and examined the regulation of PtdEtn-PLD activity under certain growth, nutritional and stress...

Ngày tải lên: 22/02/2014, 07:20

... constitutively express the mRNA of four PLA2 isotypes (type IB, type V, type IV and type VI) Interestingly, addition of TG stimulated the induction of the four PLA2 isotypes in Jurkat T-cells PLA2 inhibitors ... AA interacts extracellularly was added during the spike of ionomycin In order to probe the role of different calcium channels, implicated in AAinduced calcium...

Ngày tải lên: 08/03/2014, 09:20

... changes in PLA2 activity and expression after hyperalgesia and the role of PLA2 in the synaptic transmission This study therefore, elucidated the role of PLA2, sPLA2 in particular, after orofacial pain ... is pain and how we classify orofacial pain? In: Lund JP, Lavigne GJ, Dubner R, Sessle BJ (eds) Orofacial pain : from basic science to clinical manageme...

Ngày tải lên: 10/09/2015, 08:30

... affirming the role of sPLA2 in inflammation The present finding of dense immunolabeling of sPLA2-IIA in the spinal trigeminal nucleus of the brainstem and dorsal horn of the cervical spinal cord ... previous findings of an important role of sPLA2 in nociceptive 125 Chapter Role of Group IIA sPLA2 in nociception transmission, both from the orofacial region (Y...

Ngày tải lên: 10/09/2015, 08:31

... (sPLA2)……………………… ……… 1. 3 .1 sPLA2 isozymes…………………………………………………9 1. 3 .1. 1 sPLA2-IB…………………………………………………… … 1. 3 .1. 2 sPLA2-IIA……………………………… …………………… 10 1. 3 .1. 3 sPLA2-IIC…………………… ……… ……… ………… 11 1. 3 .1. 4 ... phospholipids (Fig 1. 1 .1. ) Different isoforms of PLA2 enzymes exists, including secretory phospholipase A2 (sPLA2), calcium (Ca2+)dependent cytosolic phospholipase...

Ngày tải lên: 10/09/2015, 08:32

... cleavage of the enzyme is 1 02 Chapter Role of Group III sPLA2 in nociception and synaptic transmission in the CNS likely to occur prior to the secretion of the sPLA2 domain of the sPLA2-III into ... solution containing Ca2+, resulted in attenuation of sPLA2-III induced exocytosis.sPLA2-III also induced rise in [Ca2+]i, and this effect was abolished in cells whi...

Ngày tải lên: 10/09/2015, 08:34

... role of miRNAs with respect to the complicated regulation of the transcriptome and signal transduction Although miRNAs and well-established cell signaling pathways have been the subject of recent ... have focused upon the role of miRNAs in regulatory network of various cell signaling pathways We summarize the current knowledge of the interdependence of miRNA...

Ngày tải lên: 14/02/2014, 19:20