FUNCTION AND REGULATION OF CALCIUM INDEPENDENT PHOSPHOLIPASE a2 IN THE ATTENUATION OF PAIN IN MICE

... sometimes 2.1 Orofacial pain Orofacial pain is defined as pain within the structures of the oral cavity and face, usually of a diffuse pattern” (Zwemer, 1998) Orofacial pain includes pain disorders ... revealed that pain is one of the main reasons that patients look for medical attention and that the lifetime prevalence of pain indications such as back pain, chest pain, headache and joint pain is around ... neuropathic pain in mice, further highlighting the involvement of DHA and its metabolites in mediating neuroinflammation and pain (Yacoubian and Serhan, 2007; Nakamoto et al., 2010; Xu et al., 2013) Pain...

Distribution and role of calcium independent phospholipase a2 in the brain

... is the cessation of blood flow to the brain and disruption of metabolic demand During early stages of ischemia (within min), inositolcontaining glycerophospholipids are the main source of the increase ... define the physiological role of sPLA2 and ascertain its contribution in the inflammatory process 1.2 cPLA2 isoforms The cPLA2 family consists of six intracellular enzymes, cPLA2α, cPLA2β, cPLA2γ, ... class of phospholipase A2 (PLA2) that does not require calcium for its activity is the cytosolic calcium- independent PLA2 (iPLA2) In brain tissues, the basal expression and activity of iPLA2 is...

Tài liệu Báo cáo khoa học: Calcium-independent phospholipase A2 mediates proliferation of human promonocytic U937 cells pptx

... iPLA2-VIA mediates U937 cell proliferation M A Balboa et al cPLA2 activity (% hydrolysis) A 0.0 0.2 0.4 0.6 0.8 1.0 [Pyrrophenone] (µM) B Number of cells × 105 iPLA2-VIA by treatment of cells ... the Ca2+dependent PLA2 activity of U937 cell homogenates The cell membrane assay was utilized (B) Time course of the effect of pyrrophenone on the proliferative capacity of U937 cells The cells ... involvement of iPLA2, but not cPLA2a, in U937 cell proliferation Owing to the lack of speciﬁcity of BEL in cell-based assays [28], additional pharmacological evidence for the involvement of iPLA2 in U937...

Báo cáo khoa học: Calcium-independent phospholipase A2-mediated formation of 1,2-diarachidonoyl-glycerophosphoinositol in monocytes potx

... increased rate of arachidonatephospholipid remodeling Lack of involvement of group IV and group VI phospholipase A2 in remodeling and increased susceptibility of proliferating T cells to CoA-independent ... concentrations that involves the direct acylation of both the sn-1 and sn-2 positions of PtdIns 1,2-Diarachidonoyl-glycerophosphoinositol Results Initial incorporation of [2H]AA into PtdIns When monocyte ... incorporation of exogenous [2H]AA into PtdIns To directly study the role of deacylation–reacylation reactions in the incorporation of AA into PtdIns, we conducted experiments in the presence of the...

Báo cáo khoa học: Protein transport in organelles: The composition, function and regulation of the Tic complex in chloroplast protein import pptx

... in protein protein interaction are blue and the dehydrogenase domains of Tic3 2 and Tic6 2 are shown in green Tic1 10 contains two transmembrane domains at the proximal N-terminus The topology of ... surprising to ﬁnd proteins with redox-active domains as Tic constituents Up to now, the ‘regulon’ of the Tic complex comprises three proteins: Tic6 2, Tic3 2 and Tic5 5 The former two proteins belong ... and the two bona ﬁde dehydrogenases Tic3 2 and Tic6 2 at the Tic complex holds the intriguing possibility of a Fig Schematic model of the proposed regulatory signals sensed by the Tic complex and...

Báo cáo khoa học: Function and regulation of ABCA1 – membrane meso-domain organization and reorganization pptx

... distribution of FEBS Journal 278 (2011) 319 0–3 203 ª 2011 The Authors Journal compilation ª 2011 FEBS 3193 Function and regulation of ABCA1 K Nagao et al Table Effects of mutations on the functions of ABCA1 ... re-secreted The internalized ABCA1 and apoA-I were reported to co-localize within late endosomes, and ABCA1 rapidly 3194 Fig Membrane meso-domain organization and reorganization by ABCA1 (A) Two proposed ... FEBS 3195 Function and regulation of ABCA1 K Nagao et al ABCA1 whereas direct binding of photoactive cholesterol to ABCA1 could not be detected [74]; however, analysis of the functions of ABCA7...

Characterization of the function and regulation of cullin ring e3 ubiquitin ligases

... characterized is the Cullin RING E3 ubiquitin ligases 1.5 Cullin RING E3 Ubiquitin Ligases Cullins form an evolutionarily conserved gene family They were first discovered as mediators of ubiquitin dependent ... promoting the binding of CAND1 to the CRL complex (Cope and Deshaies, 2003; Bosu and Kipreos, 2008) CAND1 only binds to the unneddylated form of cullin proteins Furthermore, the binding of CAND1 and ... Cullin RING ubiquitin ligases (CRLs) constitute the largest family of cellular ubiquitin ligases with diverse cellular functions CRLs comprise of seven homologous cullin- based complexes The cullin...

Molecular function and regulation of the bax associating protein MOAP 1

... MOLECULAR FUNCTION AND REGULATION OF THE BAX- ASSOCIATING PROTEIN MOAP- 1 Fu Nai Yang INSTITUTE OF MOLECULAR AND CELL BIOLOGY NATIONAL UNIVERSITY OF SINGAPORE 2007 MOLECULAR FUNCTION AND REGULATION ... pore 35 1. 3.3.3 Channel formation 38 ii 1. 4 THE MULTIDOMAIN PRO-APOPTOTIC PROTEIN BAX AND BAK 41 1.4 .1 Bax plays dominant role over Bak 41 1.4.2 Regulation of Bax function 42 1. 5 REGULATION OF MITOCHONDIA-DEPENDENT ... apoptotic stimuli 11 4 3.2.8 MOAP- 1 is a key short-lived protein required for Bax function in mitochondria 11 7 3.2.9 Conclusions 12 0 3.2 .10 Acknowledgement 12 1 DISCUSSION 12 2 4 .1 MOAP- 1 IS A MITOCHONDRIAL...

Tài liệu Báo cáo Y học: Characterization and regulation of yeast Ca2+-dependent phosphatidylethanolamine-phospholipase D activity docx

... cytosolic and membranebound PtdEtn-PLD activity Cytosolic and membrane-bound fractions were prepared as described in Materials and methods PtdEtn-PLD activity measured without addition of EDTA, ... characterized the cytosolic and membrane-bound forms of yeast PtdEtn-PLD and examined the regulation of PtdEtn-PLD activity under certain growth, nutritional and stress conditions MATERIALS AND METHODS ... highly likely that yeast PtdEtn-PLD is encoded by a non-HKD gene and may thus represent a novel PLD gene family A prokaryotic PLD activity similar to yeast PtdEtn-PLD that was identiﬁed recently...

Báo cáo Y học: Role of three isoforms of phospholipase A2 in capacitative calcium inﬂux in human T-cells pot

... constitutively express the mRNA of four PLA2 isotypes (type IB, type V, type IV and type VI) Interestingly, addition of TG stimulated the induction of the four PLA2 isotypes in Jurkat T-cells PLA2 inhibitors ... AA interacts extracellularly was added during the spike of ionomycin In order to probe the role of different calcium channels, implicated in AAinduced calcium in ux, we employed, tyrphostin A9 ... of BSA during the peak response of AA Ó FEBS 2002 Capacitative calcium in ux in human T-cells (Eur J Biochem 269) 5561 Fig Eﬀects of arachidonic acid (AA) on ionomycin-induced calcium in ux in...

Role of phospholipase a2 in orofacial pain and synaptic transmission 4

... changes in PLA2 activity and expression after hyperalgesia and the role of PLA2 in the synaptic transmission This study therefore, elucidated the role of PLA2, sPLA2 in particular, after orofacial pain ... is pain and how we classify orofacial pain? In: Lund JP, Lavigne GJ, Dubner R, Sessle BJ (eds) Orofacial pain : from basic science to clinical management ; the transfer of knowledge in pain research ... depletion of Ca2+ by thapsigargin and incubation in zero external Ca2+ resulted in attenuation of lysoPI induced exocytosis, indicating that exocytosis was dependent on the integrity of lipid rafts and...

Role of phospholipase a2 in orofacial pain and synaptic transmission 3

... affirming the role of sPLA2 in inflammation The present finding of dense immunolabeling of sPLA2-IIA in the spinal trigeminal nucleus of the brainstem and dorsal horn of the cervical spinal cord ... previous findings of an important role of sPLA2 in nociceptive 125 Chapter Role of Group IIA sPLA2 in nociception transmission, both from the orofacial region (Yeo et al 2004) and the rest of the ... showed bands at 14 kDa of sPLA2-IIA in homogenates from brainstem and spinal cord and a second band at approximately 30 kDa in the spinal cord The second band could be due to dimerization of sPLA2-IIA...

Role of phospholipase a2 in orofacial pain and synaptic transmission 1

... (sPLA2)……………………… ……… 1. 3 .1 sPLA2 isozymes…………………………………………………9 1. 3 .1. 1 sPLA2-IB…………………………………………………… … 1. 3 .1. 2 sPLA2-IIA……………………………… …………………… 10 1. 3 .1. 3 sPLA2-IIC…………………… ……… ……… ………… 11 1. 3 .1. 4 ... phospholipids (Fig 1. 1 .1. ) Different isoforms of PLA2 enzymes exists, including secretory phospholipase A2 (sPLA2), calcium (Ca2+)dependent cytosolic phospholipase A2 (cPLA2), and Ca2+-independent phospholipase ... as orofacial pain (McNeill and Dubner 20 01) Orofacial pain is one of the most widespread pains experienced by an individual and is different from the pain sensation derived from the rest of the...

Role of phospholipase a2 in orofacial pain and synaptic transmission 2

... cleavage of the enzyme is 1 02 Chapter Role of Group III sPLA2 in nociception and synaptic transmission in the CNS likely to occur prior to the secretion of the sPLA2 domain of the sPLA2-III into ... solution containing Ca2+, resulted in attenuation of sPLA2-III induced exocytosis.sPLA2-III also induced rise in [Ca2+]i, and this effect was abolished in cells which had been pre-incubated with ... lipid domains 103 Chapter Role of Group III sPLA2 in nociception and synaptic transmission in the CNS on cellular membranes and a rise in [Ca2+]i concentration, through influx via Ca2+ channels...

Tài liệu Báo cáo khoa học: miRNAs and regulation of cell signaling pptx

... role of miRNAs with respect to the complicated regulation of the transcriptome and signal transduction Although miRNAs and well-established cell signaling pathways have been the subject of recent ... have focused upon the role of miRNAs in regulatory network of various cell signaling pathways We summarize the current knowledge of the interdependence of miRNA and cell signaling pathways, which ... dme-miR-8 Wnt signaling pathway Fig Involvement of miRNAs in various signaling cascades Many miRNAs are under the control of various conserved signaling pathways and in turn regulate components of these...

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