Tài liệu Ribosome pptx
... thành phần protein ribosome đóng vai trò giá đỡ giúp tăng khả xúc tác phản ứng rARN Nói cách khác, ribosome coi ribozyme xúc tác hình thành liên kết peptide Cấu trúc chức ribosome, phân tử liên ... Toàn cấu trúc ribosome 70S tái vào năm 2001 công trình Yusupov et al tạp chí Science[4].đây cấu trúc riboxom tế bào nhân sơ tế bào nhân thực độ lắng 40, 60, 810S Ribosome tự do: Ribosome...
Ngày tải lên: 14/12/2013, 00:15
... Purification of four pulchellin isoforms from A pulchellus seeds Using a combination of affinity, ion exchange and chromatofocusing chromatography, four pulchellin isoforms were isolated from A pulchellus ... II, III and IV and refers to each pulchellin Characterization of four pulchellin isoforms isoform (P I, P II, P II and P IV) The A-chain of...
Ngày tải lên: 18/02/2014, 16:20
... structure / w 16 1 ± )4 8 ± )2 0 16 0 )4 8 14 )4 0 ± 11 )9 0 ± 21 )6 4 )4 3 )6 0 13 12 8 ± 12 )6 3 ± 30 )7 0 13 0 )6 0 17 48 ± 68 )1 2 8 ± 93 )1 2 0 45 18 0 51 )4 ± 13 90 58 )2 2 ± 46 )6 2 ± 10 5 )6 3 )4 0 )6 0 10 4 )1 3 5 ... )1 3 5 ± 73 )8 7 )1 7 0 44 )2 3 ± 16 )6 3 )3 5 23 13 5 ± )1 1 0 ± 17 )7 5 1...
Ngày tải lên: 07/03/2014, 05:20
Báo cáo khoa học: Characterization of the tRNA and ribosome-dependent pppGpp-synthesis by recombinant stringent factor from Escherichia coli pot
... 6E) From these results it can be concluded that tRNAs were bound in classical states with the tRNAMet bound f in the P-sites of the 30S and 50S subunits and the tRNAPhe bound in the A-site of the ... SF and tRNA were added simultaneously to the reaction mixtures, in agreement with the results presented by Wendrich et al [7] tRNA and ribosome-dependent syn...
Ngày tải lên: 07/03/2014, 16:20
Báo cáo khoa học: Pulchellin, a highly toxic type 2 ribosome-inactivating protein from Abrus pulchellus Cloning, heterologous expression of A-chain and structural studies ppt
... Tyr158, Glu2 12, Arg215 and Trp246 in ricin) and another ve conserved residues (Asn 72, Arg 124 , Gln160, Glu195 and Asn196 in abrin -a and abrin-c and Asn78, Arg134, Gln1 72, Glu208 and Asn209 in ricin) ... Journal 27 2 (20 05) 120 1 121 0 ê 20 05 FEBS 120 3 Pulchellin A- chain: cloning and structural studies A A L C Silva et al B C Fig (A) A- chain expression anal...
Ngày tải lên: 07/03/2014, 16:20
Báo cáo khoa học: Isolation and enzymatic characterization of lamjapin, the first ribosome-inactivating protein from cryptogamic algal plant (Laminaria japonica A) ppt
... protein synthesis in the cell-free system of rabbit reticulocyte The protein synthesis decreased gradually with the increment of lamjapin in the reaction mixture The IC50 (the concentration of ... problem, the phenyl-Sepharose CL-4B column chromatography was chosen as the first step and it separated efficiently the target proteins from the inhibitory substan...
Ngày tải lên: 08/03/2014, 10:20
Các ribosome - bộ máy tổng hợp protein pptx
... Mô hình ribosome hoạt đông chức máy tính xây dựng Đây mô hình ribosome vi khuẩn với hình dạng tổng thể Ribosome sinh vật nhân thật có cấu hình tương tự Mỗi tiểu phần ribosome phức hệ protein ... yếu cấu trúc chức ribosome Các protein, chiếm phần lớn phần bao ribosome, giúp thay đổi cấu hình không gian phân tử rARN phân tử thực vai trò xúc tác trình dịch mã Các ARN ribos...
Ngày tải lên: 12/03/2014, 06:20
tRna & ribosomes
... recognized tRNAs only by their acceptor stems tRNA acceptor stem tRNA O O P O (terminal 3’nucleotide of appropriate tRNA) CH2 O− There are families of Aminoacyl -tRNA Synthetases: Class I & Class ... amino acid & tRNAs coding for that amino acid Identity elements: tRNA domains recognized by an aaRS Most identity elements are in the acceptor stem & anticodon loop anticodon...
Ngày tải lên: 13/03/2014, 19:07
Structure of the S15,S6,S18-rRNA Complex:Assembly of the 30S Ribosome Central Domain
... S11 binds to complete the “core” of the central domain (13) Finally, the remainder of the central domain rRNA assembles onto the core, forming the functional elements of the 30S ribosomal P-site ... of the central domain RNA are not involved in directing assembly of the domain but rather are displayed on the surface of a preassembled RNP core He...
Ngày tải lên: 13/03/2014, 19:08
Báo cáo khoa học: Interactions of elongation factor EF-P with the Escherichia coli ribosome doc
... position of the EF-P protein on the Escherichia coli 70S ribosome and its subunits Binding of labeled EF-P to ribosomes To study the binding site of EF-P on ribosomes, we first ascertained that ribosomes ... to the amount of EF-P bound, to the area of the peaks, which is related to the total amount of the ribosome population in that region of the...
Ngày tải lên: 16/03/2014, 06:20
Báo cáo khoa học: Isolation, characterization, sequencing and crystal structure of charybdin, a type 1 ribosome-inactivating protein from Charybdis maritima agg. potx
... and structural analysis of charybdin, a novel 29-kDa type ribosome-inactivating protein, from bulbs of the white variety of C maritima agg Ribosome-inactivating protein from C maritima agg caused ... characterization and crystallization of charybdin The yield of the purified protein was 15 0–200 mg protein per 10 0 g of bulbs Charybdin appeared as a...
Ngày tải lên: 16/03/2014, 14:20
Báo cáo Y học: Ribosome-associated factor Y adopts a fold resembling a double-stranded RNA binding domain scaffold potx
... with similarity to the double-stranded RNA- binding domain and (b) proteins with similarity to the C-terminal domain of glycyl-tRNA synthetase The first group includes exclusively RNA- binding proteins ... can be compared with over 50 homologs found in known bacterial genomes, and in Arabidopsis thaliana and Spinacia oleracea plant sequences A search using FASTA [34] and BLAST [3...
Ngày tải lên: 17/03/2014, 10:20
Báo cáo Y học: Tag-mediated isolation of yeast mitochondrial ribosome and mass spectrometric identification of its new components ppt
... MRPL36 MRPL32 MRPL39 YPL183w-A YMR024w YDR322w YLR439w YPL173w YNL252c YKR006c YLR312w-A YKR085c YGR076c YDR462w YBR282w YCR071c YNR022c YPR100w YKL167c YKL138c YBR268w YMR225c MRPL3 MRPL35 MRPL4 ... 9.96 ypg? ypg? ? ypgypg- 19.4 10.51 ypg- 25.4 10.74 ypg- pI Disruptantb Referencec (small subunit) YHL004w MRP4 YNL137c YBR251w YKL003c YJR113c YMR158w YBR146w YDR041w YNL306w YNR036c YNL081c YPR...
Ngày tải lên: 17/03/2014, 10:20
Báo cáo khoa học: An internal ribosome entry site mediates the initiation of soluble guanylyl cyclase b2 mRNA translation potx
... transcription and scanning translation It also suggests that the initiation of sGC b2 translation is through an alternative mechanism that is less efficient than scanning translation The 5¢-UTR of sCG b2 mRNA ... of the sGC b2- subunit We found that sGC b2 translation is mediated by an IRES This is the first example of an IRES being identified in one of...
Ngày tải lên: 23/03/2014, 07:20