Identification of the Pba1 and Pba2 Binding Sites on 20S Core Particle Intermediates

... P9=69G*69L67E*7:J@EC?=78N*@=*B7@8=*=6E77*@B=7EG@=9H7*ME?=7@8?<78*6@H7*;77G* 9>7G=9D97>b*=67*9<<:G?ME?=7@8?<7N*=67*=6C<?ME?=7@8?<7N*@G>*=67*=78=78*8M7A9D9A* ME?=7@8?<7R* * * * * V.* V.* =6787*<:=@G=8*Q7E7*=E@G8D?E<7>*9G=? *pba1 Δ *?E *pba2 Δ *8=E@9G8*=6@=*@B8?*A?G=@9G7>*@* >7B7=9?G*?D*=67*RPN4*L7G7R**3MGXM*98*@*=E@G8AE9M=9?G*D@A=?E*=6@=*E7L:B@=78*...

Ngày tải lên: 24/08/2014, 13:48

... addition of the second electron (sq/hq transition). The tendency of the protein to aggregate at negative potentials, along with the low potential for the semiquinone/hydroquinone couple of the ... concentration of the FMN domain is increased indicates that the reaction rate is controlled by some process other than collision of the two flavin -binding domains. Discu...

Ngày tải lên: 23/03/2014, 21:20

... zygomatic arch and the inferior border of the mandible (Fig. 1). Acceler- ometers were fastened on the forehead as a reference and on the middle point of the chin concavity of the mandible with ... 43.3% of clenching, and 24.4% of tap- ping activities based on the count of events; whereas 56.8% of grinding, 37.4% of clenching, and 5.8% of tap- pin...

Ngày tải lên: 11/08/2014, 20:20

... used for the search. c Position of the center of the putative binding sites relative to the ORF start position. d Sequences of putative binding sites. e Changes in binding free energy and standard ... rep- resent the actual binding site of the slr1667–slr1668 operon. The binding site upstream of the operon has the lowest DDG A total value of those...

Ngày tải lên: 30/03/2014, 10:20

... of E2020 and Trp279 of AChE at the peripheral anionic-site and between the benzyl ring of E2020 and Trp84 of AChE at the bottom of the gorge. However, two of the conclusions drawn from the crystallographic ... Trp82 in the active-site and Trp277 in the peripheral anionic site. To further examine the role of the dimension and the microenvironment...

Ngày tải lên: 30/03/2014, 20:20

... within G6 by the physi- cal coupling of the two calcium binding sites within G2 and G6. Additional calcium binding in other domains then result in the complete opening and activation of the gelsolin ... coordination of calcium sites as is proposed for IIG2 and IIG6, in addition to the general opening of the molecule. Cooperation of the sites would also...

Ngày tải lên: 31/03/2014, 01:20

... polarization is further evidence that the functions of Vrp1p in endocytosis, cytokinesis, and growth at elevated temperature, on the one hand, and in cortical actin-patch polarization, on the other hand, ... level of the untagged pro- teins, but we expect that the relative expression level of the tagged proteins is indicative of that of the equival- ent untagge...

Ngày tải lên: 18/02/2014, 16:20

... reproducing the HSA sequence. A v ery strong influence of phosphate ions on the kinetics of Ni(II) interaction was found. This study demonstrates the limitations of short peptide modelling of Cu(II) and ... solution by the rest of the protein. There is no correlation between the complex stability and the rate of its formation. The Ni(II) for Cu(II) exchange ra...

Ngày tải lên: 22/02/2014, 04:20

... to the s-site only, and inactive mR1 4b , with d-ATP bound to the s- and a -sites [2]. Addition of l-ATP should lead to saturation of the a-site and complete conversion to mR1 4b , leading to the ... R1 4b formation, thus accounting for the biphasic curves observed. The changes in the curves in Fig. 2 as a function of CDP concentration are consis- tent with the kn...

Ngày tải lên: 07/03/2014, 17:20

... yields depend on the spinlabel position and the concentration of the labelled lipids. The relation of these results to the depth of the fluorophore can be expressed in the following equation: Z cF ¼ ... quencher 1 and the deep quencher 2. The two-dimensional quencher concentration in the bilayer is expressed as C, calculated as the ratio of the mole fracti...

Ngày tải lên: 08/03/2014, 09:20