IDENTIFICATION OF PUTATIVE TARGETS OF NKX2-5 IN XENOPUS LAEVIS USING CROSS-SPECIES ANNOTATION AND MICROARRAY GENE EXPRESSION ANALYSIS
Báo cáo khoa học: Identification and characterization of B¢¢-subunits of protein phosphatase 2 A in Xenopus laevis oocytes and adult tissues Evidence for an independent N-terminal splice variant of PR130 and an extended human PR48 protein pot
... proteins from an in vitro transcribed RNA injected into X. laevis oocytes. High expression is obtained as a consequence of inserting the cDNAs between the 5¢ -and3 ¢-UTR of b-globin, a protein that ... encoding XPR130, XN73 and XPR70 using a GST Gene Fusion System and subsequently inserted into the Xenopus expres- sion vector pGemHE [37]. This vector is designed to generat...
Ngày tải lên: 08/03/2014, 08:20
Báo cáo y học: "ExpressionPlot: a web-based framework for analysis of RNA-Seq and microarray gene expression data" doc
... conceived of and wrote the software and the manuscript. TM helped in its design and coordination and in drafting the manuscript. Both authors read and approved the final manuscript. Competing interests The ... analysis of gene expres- sion and RNA processing data. The back end offers a solution to the problem of detecting significan t changes in gene expression...
Ngày tải lên: 09/08/2014, 23:20
Báo cáo y học: "Identification of secondary targets of N-containing bisphosphonates in mammalian cells via parallel competition analysis of the barcoded yeast deletion collection" ppsx
... haploinsufficient and haploproficient genes after removal of bad tags and dubious ORFsFigure 1 Venn diagram of numbers of haploinsufficient and haploproficient genes after removal of bad tags and ... identified novel targets of nitrogen-containing bisphosphonates, such as tubulin cofactor B and ASK/DBF4 (Activator of S-phase kinase). The up-regulation of tubulin cof...
Ngày tải lên: 09/08/2014, 20:20
Báo cáo y học: " Identification of novel transcripts with differential dorso-ventral expression in Xenopus gastrula using serial analysis of gene expression" pptx
... Screening of FGF target genes in Xenopus by micro- array: temporal dissection of the signaling pathway using a chemical inhibitor. Genes Cells 2004, 9:749-761. 19. Peiffer DA, Von Bubnoff A, Shin ... Niu R, Khandpur R, Martinez M, Shin Y, Koide T, Cho KW, Kitayama A, Ueno N, Chandraratna RA, Blumberg B: Global analysis of RAR-responsive genes in the Xenopus neu- rula using...
Ngày tải lên: 14/08/2014, 21:20
Báo cáo khoa học: Modulation of the Arabidopsis KAT1 channel by an activator of protein kinase C in Xenopus laevis oocytes potx
... activates PKC and regulates the activity of other proteins involved in carcinogenesis and metastasis, as well as in cell growth, development, survival and apoptosis [29– 33]. DAG, generated from PI in the ... we used the Xenopus laevis oocyte protein kinase C to identify the target sites of calcium-dependent protein kinases. KAT1 expressed in Xenopus oocytes was inhibi...
Ngày tải lên: 22/03/2014, 21:21
Báo cáo khoa học: Interaction of 42Sp50 with the vegetal RNA localization machinery in Xenopus laevis oocytes ppt
... presence of 42Sp50 in nucleus and cytoplasm indicates that it might be a shuttling protein with a function in the nuclear export of localizing mRNAs. To determine whether 42Sp50 is capable of shuttling, we ... stage of the transport process they join the localization complex. Interestingly, Stau- fen has been found to interact with kinesin in X. laevis oocytes, and might t...
Ngày tải lên: 23/03/2014, 03:20
Báo cáo khoa học: Identification of alternative promoter usage for the matrix Gla protein gene Evidence for differential expression during early development in Xenopus laevis doc
... MGP as a possible target gene. Accord- ingly, other genes involved in bone and cartilage metabolism, including type I and II collagen genes [24,25] and the rat osteocalcin gene [26], have been found ... regulator and the vertebrate homologue of the Drosophila pro- tein zfh-1 [19], a factor containing both zinc finger and homeodomain motifs. It is a 124 kDa DNA-binding protein...
Ngày tải lên: 30/03/2014, 16:20
Báo cáo y học: "Association of the T+294C polymorphism in PPAR δ with low HDL cholesterol and coronary heart disease risk in women"
... improvement of metabolic traits characterized by a rise of HDL and lowering of LDL, triglycerides, and insulin [5]. In addition, transgenic expression of an activated PPARδ in mice conferred ... silver staining on polyacrylamid gel. Statistical Analysis Allele frequencies were determined by gene counting and compared using the א² test. The data was analysed us...
Ngày tải lên: 31/10/2012, 17:03
Tài liệu Báo cáo Y học: Induction of (2¢)5¢)oligoadenylate synthetase in the marine sponges Suberites domuncula and Geodia cydonium by the bacterial endotoxin lipopolysaccharide docx
... segment within amino acid 104 and amino acid 158 of the murine enzyme [53]; in S. do muncula a related stretch has been found between amino acid 76 and amino acid 125. The polyA-related domain, found in ... c). DISCUSSION Inhibition of cell growth, apoptosis and inhibition of protein synthesis are ways of protection of metazoan organisms against death caused by microbes. T...
Ngày tải lên: 21/02/2014, 15:20
Báo cáo khoa học: Role of the C-terminal extension in a bacterial tyrosinase Michael Fairhead and Linda Thony-Meyer doc
... motif Arg40 Cys84 Tyr349 Tyr347 Lys370 Ala36 Ala36 Ala36 Val357 Phe518 B Laccase Tyrosinase β -sheet protein Fig. 1. Overview of the tyrosinase gene and surrounding genes in the genome of V. spinosum. (A) Showing the tyrosinase gene and those in its immediate vicinity in the ... domain structure of the V. spino- sum tyrosinase in more detail, we determined protein stabi...
Ngày tải lên: 06/03/2014, 11:20