EXPRESSION AND FUNCTION OF THE PRL FAMILY OF PROTEIN TYROSINE PHOSPHATASE

... regions surrounding the active sites of the proteinases and their expansin counterparts [4], and their comparable location enhances the significance of the sequence similarities. Other functional amino ... parasites of the genus Giardia are one of the earliest lineages of eukaryotic cells, and the Giardia protease is the earliest known branch of the cathepsi...

Ngày tải lên: 08/03/2014, 22:20

... level only at the RNA level. In order to confirm t hat the effect of GPR30 on the regulation of cofactor and g rowth was due to GPR30 protein expression, we measured the effects of GPR30 fused ... 6C). The re sult suggests that the measured effects on growth and the expression of cofactor were due to GPR30 protein expression. Discussion Molecular discriminati...

Ngày tải lên: 16/03/2014, 18:20

... also observed in the rate of degradation of the remaining mutant protein (20% left of the protein) vs. the wild type (40% left of the protein) at 3 h of chase (Fig. 7A,B). We also analyzed the rate of degradation of ... fast rate of degradation. The results impli- cate a prominent role for the proteasome pathway in down regulation and basal turnover of...

Ngày tải lên: 20/02/2014, 03:20

... Ets -family is involved in the regulation. The members of the Ets -family of transcription factors bind to specific purine-rich sequences with a core motif of GGAA/T and control the expression of ... plasmid. The assays were carried out in triplicate, and the error bars indicate the standard deviation. Fig. 8. Effects of mutations in the Ets- and GATA-binding...

Ngày tải lên: 21/02/2014, 03:20

... Leu508 of the x-linker and residues Phe484, Phe499 and Phe503 of the b¢ domain. Towards the middle of the x-linker, Tyr364 of ERp57 (and Val511 of ERp72) fit into a small hydrophobic pocket below the C-terminus ... between the a and AB C E D Fig. 3. A cavity on the a1–a3 surface of the b¢ domain defines the ability of the PDI to bind hydrophobic stretche...

Ngày tải lên: 15/03/2014, 23:20

... signal into the nucleus, a genetic reprogramming occurs that leads, on the one hand, to a decrease in the expression of house- keeping genes and protein synthesis and, on the other hand, to an ... forms of stress highlighting, in particular, those in which the Yap family of basic-leucine zipper (b-ZIP) transactiva- tors play a role. The Yap protein family The...

Ngày tải lên: 30/03/2014, 16:20

... and expressed as the fold increase relative to that of the band for Noxo1c in the absence of PMA. R. Takeya et al. Expression and function of Noxo1c FEBS Journal 273 (2006) 3663–3677 ª 2006 The ... as the insert region is located between the pair of primers (Fig. 2A). The lengths of the PCR products of Noxo1b and Noxo1c were 225 and 240 bp, respectively...

Ngày tải lên: 19/02/2014, 06:20

... ST fibroblasts and MΦs at baseline and was further increased by TNFα or IL1β stimulation. pPaxillin and pPLCγ were upregulated in RA versus OA and ND lining cells and sublining MΦs. Activation of the FAK family ... fibroblasts. The pro-inflammatory cytokines TNFα and IL1β further activated pFAK, pPyk2 and pSrc expression up to 120 min. The activation of the FAK-S...

Ngày tải lên: 09/08/2014, 10:21

... this RNR and that of other class 1 RNRs or, instead, is a result of the interaction of the R2F with the inactivated (oxidized) form of the R1E. We consider the first option unlikely. Under these ... Because of high expression from the tac promotor, the proposed function of man- ganese in the transcriptional regulation of the nrd operon [35] may not be cons...

Ngày tải lên: 15/02/2014, 01:20

... particular, p21 is a direct target of SREBP [20]. The role of SREBP-1a in the regulation of cell growth and the cell cycle might be biphasic and complex, and needs to be further investigated. SREBP is ... conditions of overnutrition, SREBP-1c expression is elevated, and consequently, the levels of nuclear SREBP-1c protein and lipogenesis are enhanced in the l...

Ngày tải lên: 18/02/2014, 13:20