... Isolation and identification of a novel human parechovirus. J Gen Virol 2004, 85:391-398. 11. Abed Y, Boivin G: Molecular characterization of a Canadian human parechovirus (HPeV)-3 isolate and ... Ger- many). The first amplification stage (20 cycles, 50 μl reac- tion volumes) used 300 nM of primers CTCGTAGACTGCGTACGATG and GACGATGAGTACT- GATCGC at 56°C annealing temperature wi...
Ngày tải lên: 20/06/2014, 01:20
... are seen on the rate of heat release. With advancement of injection by 3 degrees, the peak rate of heat release is at 357 degree crank angle and on retarding by 3 and 6 degrees, the peak heat ... as ratanjyot, belongs to the family of Euphorbiaceae. It is a quick yielding plant that survives in degraded, barren, forest land and draught prone areas and is cultivated as hedg...
Ngày tải lên: 05/09/2013, 16:11
A study on cultural obstacles to the teaching and learning of speaking skills in the classroom of grade 10 at nguyen tat thanh high school
... learning language.” (Eli Hinkel, 1999) In fact, language is part of a culture; language is deeply embedded in a culture; language and culture cannot be separated, and we cannot teach a language ... understand the what, when, why, and in which way to speak. In order for a learner to speak English fluently, apart from a limited amount of grammar and vocabulary, teachers mu...
Ngày tải lên: 07/09/2013, 13:00
Tài liệu THE ENCYCLOPEDIA OF HOLLYWOOD: AN A TO Z GUIDE TO THE STARS, STORIES, AND SECRETS OF HOLLYWOOD docx
... both of her brothers, rivaling John as a Broadway star in her early years and later rivaling Lionel as a character actor in her later years. She was known as “The First Lady of the American Theater” ... Arbuckle was accused of rape and manslaughter. The scandal rocked Hollywood and destroyed Arbuckle’s career, despite the fact that (after three trials) Arbuckle was acquitted...
Ngày tải lên: 19/02/2014, 10:20
Báo cáo khoa học: A role for serglycin proteoglycan in granular retention and processing of mast cell secretory granule components ppt
... protein was detected as early as after 5 days of culture, and a maximal plat- eau of storage was already seen at day 12. Both pro- CPA and mature CPA were detected in SG + ⁄ + cells. A dramatically ... sodium acetate (pH 4.0) at a flow rate of 0.5 mLÆmin )1 . Fractions (0.5 mL) were collected and analyzed for 35 S radioactivity. As an internal standard, 200 lL of a mixtur...
Ngày tải lên: 07/03/2014, 11:20
Báo cáo khoa học: Functional expression of olfactory receptors in yeast and development of a bioassay for odorant screening docx
... (5¢-AG CTGCCTGCAGGTCGACTCTAGAGGATCCTAACCAA TTTTGCTGCC-3¢); for OR17-40 (5¢-CGTCAAGGAG AAAAAACCCCGGATCTAAAAAATGGAGCAGAAAC TCATCTCTGAAGAGGATCTG-3¢) and (5¢-GCATG CCTGCAGGTCGACTCTAGAGGATCTCAAGCCAGT GACCGCCTCCC-3¢); ... was performed on DNAse- treated RNA extracts. Primers used for RT-PCR were: for the I7 OR (5¢-CGTCAAGGAGAAAAAACCCCGGATCT AAAAAATGGAGCGAAGGAACCACAG-3¢) and (5¢-AG CTGCCTGCAGGTC...
Ngày tải lên: 07/03/2014, 16:20
Báo cáo khoa học: Targeting mechanism of the retinoblastoma tumor suppressor by a prototypical viral oncoprotein Structural modularity, intrinsic disorder and phosphorylation of human papillomavirus E7 doc
... initial and final anisotropy signals. For all reactions tested, full saturation was achieved at a 1 : 1 molar ratio of titrant, indicating a 1 : 1 stoichiometry, which validates the use of a 1 ... large T antigen (SV40LT) and adenovirus E 1A (AdE 1A) also target the Rb protein and share sequence and functional conservation with the HPV E7 protein [21,22]. E7, AdE 1A and...
Ngày tải lên: 22/03/2014, 21:20
Báo cáo khoa học: A natural osmolyte trimethylamine N-oxide promotes assembly and bundling of the bacterial cell division protein, FtsZ and counteracts the denaturing effects of urea docx
... pH dependence of the urea and guanidine hydrochloride denaturation of ribonuclease A and ribonuclease T1. Biochemistry 29, 2564–2572. 62 Pace CN (1986) Determination and analysis of urea and guanidine ... trimethylamine N-oxide promotes assembly and bundling of the bacterial cell division protein, FtsZ and counteracts the denaturing effects of urea Arnab Mukherjee, Man...
Ngày tải lên: 30/03/2014, 16:20
báo cáo hóa học: " In vitro and in vivo pre-clinical analysis of a F(ab’)2 fragment of panitumumab for molecular imaging and therapy of HER1-positive cancers" pot
... characterization of panitumumab F(ab’ ) 2 fragment with an emphasis on its evaluation towards both imaging and therapeutic applications. Materials and methods Preparation of F(ab’) 2 fragments Panitumumab ... Schoonooghe S, Blanckaert P, Bacher K, Vervoort L, Coene E, et al: Biodistribution and planar gamma camera imaging of I-123- and I- 131-labeled F(ab ‘)(2) and Fab fragmen...
Ngày tải lên: 21/06/2014, 02:20
Báo cáo khoa học: "A multiplex real-time PCR for differential detection and quantification of Salmonella spp., Salmonella enterica serovar Typhimurium and Enteritidis in meats" pps
... rRNA fliC sefA S16R-F S16R -R Scom-FAM SfC-F SfC -R ST-JOE SsA-F SsA-R SE-Cy5 aggccttcgggttgtaaagt gttagccggtgcttcttctg FAM-aaccgcagcaattgacgttaccc-BHQ 1a tgcagaaaattgatgctgct ttgcccaggttggtaatagc JOE-acctgggtgcggtacagaaccgt-BHQ 1a ggtaaaggggcttcggtatc tattggctccctgaatacgc Cy5-tggtggtgtagccactgtcccgt-BHQ 1a X80676 (415–511) AY649720 (1226–1325) L11008 (244–340) Tabl ... tube and incubat...
Ngày tải lên: 07/08/2014, 23:22