Báo cáo y học: "A novel and universal method for microRNA RT-qPCR data normalization" ppsx

... experiments and data analysis and drafted the manuscript. PVV and ADW per-formed miRNA profiling experiments. DM and FW areresponsible for MYCN ChIP-on-chip data. FS and JV con-ceived the study and ... was the case for RNU44, U47 and U75 on1q25, and RNU58A and RNU58B on 18q21, of which RNU44 and RNU58A were randomly retained for further analysis.Naturally, only those small RNA controls that ... Applied Biosystems for providing pre-release access to the Megaplex and PreAmp based miRNA profiling tech-nology, Dr Y Chen and Dr R Stallings for providing their miRNA RT-qPCR dataset. This...

Báo cáo y học: "A novel and safe technique in closed tube thoracostomy" pdf

... this study have shown that modified com-bined technique can be used safely in thoracic surgeryclinics because it reduces the incidence rates of complica-tions and TM, is easy to perform, and has ... which are commonly observed insurgical technique, and intraparenchymal-fissural loca-tions, which are common with trocar technique, as wellas pulmonary and cardiovascular injury. None of thepatients ... responsible for provision of published trialbibliographies, preparing photographs. GG was responsible for quality check-ing, coding and classification, data processing. US was responsible for projectcoordination.All...

Báo cáo khoa học: "A Fast and Accurate Method for Approximate String Search" pptx

... set show that our method im-proves upon existing methods in terms of accuracy, and particularly our method can perform better whenthe dictionary is large and when there are manyrules. Experimental ... aquery string, a dictionary of strings (vocabulary), and a set of operators, the system returns the topk strings in the dictionary that can be transformedfrom the query string by applying several ... accuracies of both methods drop,but our method is constantly better than generative.Moreover, the decrease in accuracy by our method is clearly less than that by generative. For the ex-periment...

Báo cáo hóa học: " A novel mechanical cleavage method for synthesizing few-layer graphenes" ppt

... AccessA novel mechanical cleavage method for synthesizing few-layer graphenesBuddhika Jayasena, Sathyan Subbiah*AbstractA novel method to synthesize few layer graphene from bulk graphite by mechanical ... properties are strongly affected by the method ofsynthesis [4]. While several laboratory methods tosynthesize graphene have been developed and reported,the suitability of these methods to large-scale ... article as: Jayasena and Subbiah: A novel mechanical cleavage method for synthesizing few-layer graphenes. Nanoscale Research Letters2011 6:95.Submit your manuscript to a journal and beneﬁ t...

Báo cáo khoa học: " A rapid and efficient method for studies of virus interaction at the host cell surface using enteroviruses and real-time PCR" potx

... virions, by the use of a standardcurve previously described by Jonsson et al. [33].Statistical analysesIndividual data pairs were analysed by the unpaired t test, and one-way analysis of variance ... assays, RNA extraction and real-time PCR analysis. NJ drafted and wrote the manuscript.MG developed the protocol for the binding assay, radiola-beled and purified the clinical isolate and performed ... Analysis Of The ErythrocyteReceptor For Hemagglutinating Enteroviruses. Virology 1964,22:580-590.26. Verstrepen WA, Bruynseels P, Mertens AH: Evaluation of a rapidreal-time RT-PCR assay for...

Báo cáo khoa học: " A simple and rapid method for detection of Goose Parvovirus in the field by loop-mediated isothermal amplification" pps

... ethidium bromide-stained and visualized.FQ-PCR detectionDetection was performed as described by Yang [9].Specificity of LAMP assayThe specificity of the assay was tested by using tem-plates from ... contributionsJY and YR carried out most of the experiments and wrote the manuscript, and should be considered as first authors. AC and MW critical ly revised themanuscript and the experiment design. LF, YS, ... tur-bidity due to the accumulation of magnesium pyropho-sphate, a by-product of the reaction. LAMP ampliconsin the reaction tube were directly detecte d by the nakedeye by adding 1.0 μl of a thousand-fold-diluted...

Tài liệu Báo cáo Y học: A novel meta-cleavage dioxygenase that cleaves a carboxyl-groupsubstituted 2-aminophenol Puriﬁcation and characterization of 4-amino-3-hydroxybenzoate 2,3-dioxygenase from Bordetella sp. strain 10d doc

... 4-amino-3-hydroxybenzoate cleavage. I,4-amino-3-hydroxybenzoic acid; II, 2-amino-5-carboxymuconic6-semialdehyde; III, 2-hydroxymuconic 6-semialdehyde; IV, 2,5-pyridinedicarboxylic acid. (B) Pathway ... amidol,3-hydroxyanthralinic acid, 1,2,4-trihydroxybenzene and pyrogallol) in aqueous solution were always freshlyprepared and used immediately.Effect of various compounds on the enzyme activityThe ... was analyzed by GC-MS and GC, as described below.Stoichiometry of the enzyme reaction4-Amino-3-hydroxybenzoate-dependent oxygen uptake wasmeasured with a Clark-type oxygen electrode (YellowSprings...

Báo cáo Y học: A novel DNA repair enzyme containing RNA recognition, G-patch and speciﬁc splicing factor 45-like motifs in the protozoan parasite Toxoplasma gondii potx

... slowlydividing encysted bradyzoites. When the protective immu-nity fails, as in AIDS or in transplanted patients [1], theencysted bradyzoites can transform into actively replicating and virulent ... hosts.The reactivation of encysted bradyzoites into activelyreplicating and cytolytic tachyzoites can trigger a diseasewith severe clinical syndromes leading in many cases to thedeath of the ... [16,17] and AB1157 wild-type strain (generously provided by J.B Hays,Oregon State University, USA) were transformed with thepGEX-6P-3 containing the TgDRE ORF or pGEX-6P-3alone. After isopropyl...

Báo cáo y học: "A novel hybrid aspirin-NO-releasing compound inhibits TNFalpha release from LPS-activated human monocytes and macrophages" docx

... not for citation purposes)inflammatory cytokine, TNFα from human monocyte-derived macrophages and monocytes. Cytokines arepolypeptide or glycoprotein factors that act in an auto-crine and/ or ... hybridcompounds were also investigated at 10 μM. Drugs inves-tigated were the furoxan-aspirin hybrids (3-cyanofuroxan-4-yl)methyl 2-acetoxybenzoate (B8) and (3-carbamoyl-furoxan-4-yl)methyl ... (3-carbamoyl-furoxan-4-yl)methyl 2-acetoxybenzoate (B7), theirrespective NO-free counterparts (furazans), (4-cyanofura-zan-3-yl)methyl 2-(acetoxy)benzoate (B16) and (4-car-bamoylfurazan-3-yl)methyl 2-acetoxybenzoate (B15),nitroaspirin...

Báo cáo y học: "A novel method to identify and characterise peptide mimotopes of heat shock protein 70-associated antigens" pptx

... Dublin 2, Ireland, 2Institute of Immunology & Department of Biology, National University of Ireland, Maynooth, Co. Kildare, Ireland and 3Centre for Clinical Vaccinology and Tropical Medicine, ... select/enrich and amplify ligands even from a mixture of baits. For example, intravenously administered phage displaylibrary has been successfully used for in vivo bio-panningin certain animal model systems ... vitro immuno-stimulatoryassays. ST and DD contributed to the design and supervi-sion of the immuno-stimulatory assays. TCJ and UB con-ceived and designed the methods for the isolation of thepeptide...

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