Báo cáo y học: "A novel and universal method for microRNA RT-qPCR data normalization" ppsx

... experiments and data analysis and drafted the manuscript. PVV and ADW per- formed miRNA profiling experiments. DM and FW are responsible for MYCN ChIP-on-chip data. FS and JV con- ceived the study and ... was the case for RNU44, U47 and U75 on 1q25, and RNU58A and RNU58B on 18q21, of which RNU44 and RNU58A were randomly retained for further analysis. Naturally, on...

Ngày tải lên: 14/08/2014, 21:20

... this study have shown that modified com- bined technique can be used safely in thoracic surgery clinics because it reduces the incidence rates of complica- tions and TM, is easy to perform, and has ... which are commonly observed in surgical technique, and intraparenchymal-fissural loca- tions, which are common with trocar technique, as well as pulmonary and cardiovascular injury. Non...

Ngày tải lên: 10/08/2014, 10:20

... set show that our method im- proves upon existing methods in terms of accuracy, and particularly our method can perform better when the dictionary is large and when there are many rules. Experimental ... a query string, a dictionary of strings (vocabulary), and a set of operators, the system returns the top k strings in the dictionary that can be transformed from the query string by...

Ngày tải lên: 30/03/2014, 21:20

... Access A novel mechanical cleavage method for synthesizing few-layer graphenes Buddhika Jayasena, Sathyan Subbiah * Abstract A novel method to synthesize few layer graphene from bulk graphite by mechanical ... properties are strongly affected by the method of synthesis [4]. While several laboratory methods to synthesize graphene have been developed and reported, the suitability...

Ngày tải lên: 21/06/2014, 06:20

... virions, by the use of a standard curve previously described by Jonsson et al. [33]. Statistical analyses Individual data pairs were analysed by the unpaired t test, and one-way analysis of variance ... assays, RNA extraction and real- time PCR analysis. NJ drafted and wrote the manuscript. MG developed the protocol for the binding assay, radiola- beled and purified the clinical i...

Ngày tải lên: 12/08/2014, 04:21

... ethidium bromide-stained and visualized. FQ-PCR detection Detection was performed as described by Yang [9]. Specificity of LAMP assay The specificity of the assay was tested by using tem- plates from ... contributions JY and YR carried out most of the experiments and wrote the manuscript, and should be considered as first authors. AC and MW critical ly revised the manuscript and t...

Ngày tải lên: 12/08/2014, 04:21

... 4-amino-3-hydroxybenzoate cleavage. I, 4-amino-3-hydroxybenzoic acid; II, 2-amino-5-carboxymuconic 6-semialdehyde; III, 2-hydroxymuconic 6-semialdehyde; IV, 2,5- pyridinedicarboxylic acid. (B) Pathway ... amidol, 3-hydroxyanthralinic acid, 1,2,4-trihydroxybenzene and pyrogallol) in aqueous solution were always freshly prepared and used immediately. Effect of various compounds on the enzym...

Ngày tải lên: 21/02/2014, 01:21

... slowly dividing encysted bradyzoites. When the protective immu- nity fails, as in AIDS or in transplanted patients [1], the encysted bradyzoites can transform into actively replicating and virulent ... hosts. The reactivation of encysted bradyzoites into actively replicating and cytolytic tachyzoites can trigger a disease with severe clinical syndromes leading in many cases to the death o...

Ngày tải lên: 08/03/2014, 22:20

... not for citation purposes) inflammatory cytokine, TNFα from human monocyte- derived macrophages and monocytes. Cytokines are polypeptide or glycoprotein factors that act in an auto- crine and/ or ... hybrid compounds were also investigated at 10 μM. Drugs inves- tigated were the furoxan-aspirin hybrids (3-cyanofuroxan- 4-yl)methyl 2-acetoxybenzoate (B8) and (3-carbamoyl- furoxan-4-yl)m...

Ngày tải lên: 11/08/2014, 08:22

... Dublin 2, Ireland, 2 Institute of Immunology & Department of Biology, National University of Ireland, Maynooth, Co. Kildare, Ireland and 3 Centre for Clinical Vaccinology and Tropical Medicine, ... select/ enrich and amplify ligands even from a mixture of baits. For example, intravenously administered phage display library has been successfully used for in vivo bio-panning...

Ngày tải lên: 11/08/2014, 10:23