Báo cáo y học: "Whole genome sequencing of a natural recombinant Toxoplasma gondii strain reveals chromosome sorting and local allelic variants" ppsx

... 10:R53 Open Access 2009Bontellet al.Volume 10, Issue 5, Article R53 Research Whole genome sequencing of a natural recombinant Toxoplasma gondii strain reveals chromosome sorting and local allelic variants Irene ... detection of local allelic variants and preliminary geno- type-phenotype associations. This is the first whole genome sequencing of a...

Ngày tải lên: 14/08/2014, 21:20

... 2. After SNP calling by MAQ, we applied additional filters. We required each putative SNP to have a median quality value of the variant base of at least 20 and that at least 20% of the reads ... These artifacts include cases where all sequenced variant alleles at a given position are only indicated by reads from one strand and have a lower than average base quality at the vari...

Ngày tải lên: 14/08/2014, 21:21

... for LactA, LactB, LactC, LactD, and LactE strains using Nimblegen CGS arraysMutations reported for LactA, LactB, LactC, LactD, and LactE strains using Nimblegen CGS arrays.Click here for fileAdditional ... Translation g28 3a GAA->AAA E95K LactD rph-pyrE RNase PH/orotate phosphoribosyltransferase Metabolic Δ82 bp Frameshift ppsA Phosphoenolpyruvate synthase Metabolic c28 8a ATC->A...

Ngày tải lên: 09/08/2014, 20:20

... two major pathways for the degradation of mRNA, both of which are deadenylation dependent: 5' to 3' decay and 3' to 5' decay [7]. Both pathways of mRNA decay in mammals and ... before addition of actD. DNA microarrays were scanned using an Axon 4000B scan- ner and images analyzed using Axon GenePix software (Molecular Devices, Union City, CA, USA). Microarray...

Ngày tải lên: 14/08/2014, 07:22

... broad range of uncultivatable organisms from which only a handful of contaminant-free DNA can be extracted. Finally, an important goal of the present study was to gather a large amount of genome ... field of evolutionary ecology into the post-genomic era Materials and methods DNA and RNA extraction and DNA sequencing Total RNA and genomic DNA from O. bayeri (isolate...

Ngày tải lên: 09/08/2014, 20:20

... ffuummiiggaattuuss aanndd AA oorryyzzaaee Nature 2005, 443388:: 1105-1115. 7. Fedorova ND, Khaldi N, Joardar VS, Maiti R, Amedeo P, Anderson MJ, Crabtree J, Silva JC, Badger JH, Albarraq A, Angiuoli ... model of random breakage. A surprise, however, is the fact that rearrange- ments in P. anserina and N. crassa appear mostly intra- chromosomal, as revealed by the high conservation...

Ngày tải lên: 14/08/2014, 08:21

... Van (Ǩ) × Col (ǩ), and Col (Ǩ) × Van (ǩ) were made. Each replicate cross was between individual paternal and maternal plants and each parental plant was only used once (16 Col and 16 Van plants ... differentially expressed genes, and an additional 295 genes labeled as dif- ferentially expressed by annotation were called by the HMM analysis as differentially spliced (Table S10 in Add...

Ngày tải lên: 14/08/2014, 21:20

... were residues Gly261 and Gly262. The replacement of Gly262 by Ala resulted in an inactive enzyme. Substitution of Gly261 by Ala resulted to an enzyme with lower stability and increased energy of activation. ... with the aromatic ring of Tyr269, and these unfavorable interactions could lead to a decrease of local flexibility and an increased E a value. The validity of...

Ngày tải lên: 22/02/2014, 04:20

... activity. This interaction potentially allows a crosstalk between cAMP pathways and ras-mediated pathways in cell cycle control. In addition to its many roles as an intracellular messenger, cAMP also ... expressed, while domain A again resulted in very poor cell growth and in low amounts of recombinant protein. The individual domains A and B were purified by cobalt-affinity chro...

Ngày tải lên: 31/03/2014, 23:20

... Corresponding author Abstract Background: Astroviruses infect a variety of mammals and birds and are causative agents of diarrhea in humans and other animal hosts. We have previously described ... analysis of diarrhea samples using a mass sequencing approach with the explicit goal of iden- tifying novel viruses that may be candidate causes of diarrhea. One of the stoo...

Ngày tải lên: 20/06/2014, 01:20